Secoiridoids from the roots of Gentiana straminea

Chen, Guang; Wei, Shihu; Chen, Yu

doi:10.1016/j.bse.2009.12.004

Cited by 22 publications

(17 citation statements)

References 17 publications

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“…Compounds 1-10 were isolated using chromatographic techniques from a crude extract of G. straminea following a previously described process [4]. The identification of the isolated compounds was confirmed by chromatographic (TLC, HPLC) and spectroscopic (MS, 1 H-NMR, and 13 C-NMR) methods.…”

Section: Generalmentioning

confidence: 99%

“…Gentiopicroside shows good anti-inflammatory activity and has been considered to be the main antiinflammatory constituent in Qinjiao [14,15]. In our recent research [4], ten iridoid compounds were isolated from the roots of G. straminea including three novel structures; the newly found secoiridoid 6 0 -O-(2-hydroxyl-3-O-b-D-glucopyranosyl-benzoyl)-sweroside showed stronger antiinflammatory activity than gentiopicroside with aspirin as positive control in a testing model based on inhibition of mouse's ear and paw swelling. These results indicated that gentiopicroside alone could not be responsible for the overall pharmacological actions of the herbs and that synergistic effects among the various constituents probably play significant and systematic roles.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies on G. straminea have shown that this medical herb is rich in iridoids, especially secoiridoids [4,5], well-known phytochemicals in a number of folk medicinal plants with anti-inflammatory, antibacterial, free radical scavenging, and hepatoprotective activities [2,[6][7][8][9]. Earlier reports have shown that gentiopicroside, loganic acid, sweroside, and swertiamarin are four secoiridoid constituents commonly found in G. straminea and other species in the Gentiana genus [10,11] that were identified as useful chemotaxonomical markers and also believed to be the active constituents of these folk herbs.…”

Section: Introductionmentioning

confidence: 99%

“…In China, the roots of G. straminea have been identified, together with those of Gentiana macrophylla, Gentiana crassicaulis, and Gentiana dahurica as ''Qinjiao'' in the Chinese Pharmacopoeia [12] based on the chemical characteristic that these four species have a high content (C2%) of the same major active constituent, gentiopicroside [4,13]. Gentiopicroside shows good anti-inflammatory activity and has been considered to be the main antiinflammatory constituent in Qinjiao [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

et al. 2011

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A simple and accurate HPLC-UV/MS method was developed for the simultaneous determination of ten iridoids and secoiridoids in the roots of Gentiana straminea Maxim. Separations were performed on a Kromasil-C18 column by gradient elution using methanol and water containing phosphoric acid. Analytes were identified by HPLC coupled with ESI-MS experiments. The chromatographic method was validated for selectivity, linearity, precision, limit of detection, limit of quantification, accuracy, and stability. The developed assay could be considered as a suitable quality control method for G. straminea and other "Qinjiao" herbs.

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Section: Generalmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

et al. 2011

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“…Gentiana siphonantha Maxim., a perennial herb in the Gentianaceae family, has been a traditional Chinese medicine for more than 2000 years for the treatment of rheumatoid arthritis, jaundice hepatitis, constipation, pain, and hypertension [25,26,27]. Previous studies on G. siphonantha have shown that this medical herb is rich in iridoids, especially gentiopicroside [28,29], well-known phytochemicals in a number of folk medicinal plants with anti-inflammatory, antibacterial, free radical-scavenging, and hepatoprotective activities [30,31,32,33]. The above plants have anti-inflammatory, analgesic or swelling-decreasing activities.…”

Section: Introductionmentioning

confidence: 99%

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

et al. 2018

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Background:Gentiana siphonantha Maxim. is a traditional medicine for the treatment of rheumarthritis, icterepatitis, pain and hypertension; it is rich in gentiopicroside with anti-inflammatory, antibacterial, and free radical-scavenging activities. This study was to evaluate the antidermatophyte activity of G. siphonantha on a guinea pig model in vitro and in vivo. Material andMethods: The antidermatophyte activities of 10 plants were tested by the broth microdilution method. Fractions and an extract of G. siphonantha were tested against Trichophyton mentagrophytes by the disc diffusion method. The morphological changes of T. mentagrophytes were observed. Component analysis of the n-butanol (n-BuOH) fraction was made by HPLC. Finally, the antifungal activity in an in vivo guinea pig model of dermatophytosis was examined. Results:G. siphonantha had strong antidermatophyte activity with MIC₅₀ values of 32-64 μg/mL. The n-BuOH fraction of G. siphonantha showed the most potent activity compared to the other fractions. After being exposed to the n-BuOH fraction at 80 and 160 μg/mL, the hyphae were distorted and collapsed. Gentiopicroside is the main active ingredient in the n-BuOH fraction of G. siphonantha. The lesion scores of the guinea pig model of dermatophytosis significantly declined in the 10% and 30% extract and positive control groups in comparison with the untreated control group. Periodic acid-Schiff and hematoxylin/eosin staining displayed similar results. Conclusion: The n-BuOH fraction of G. siphonantha demonstrated antidermatophyte efficacy in experimental dermatophytosis.

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Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives

Ding

Wang

Zhou

et al. 2018

Biomedical Chromatography

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Gentiana Macrophylla Radix (GMR) is officially used as traditional Chinese medicine, but easily confused with Gentianae Radix et Rhizoma (GRR) and adulterants. This study aimed to establish an HPLC method for qualitative and quantitative analysis of GMR based on characteristic components, anofinic acid and its derivatives. HPLC analysis was performed on a C column with gradient elution using acetonitrile and 0.1% phosphoric acid as mobile phase, and detected at 240 nm by conventional methodology validation. For fingerprint analysis, RSDs of relative retention times and relative peak areas of the characteristic peaks were within 0-1.10 and 0-4.08%, respectively. For determination of 2-methoxyanofinic acid, the calibration curve showed good linearity (R > 0.9999) within the test range. The RSDs of precision, repeatability and stability test did not exceed 2.46, 0.83 and 1.11%, respectively. The average recoveries were between 95.08 and 103.05% with RSDs ≤2.29%. The results showed that there was no significant difference among the four species of GMR, but there were significant differences among GMR, GRR and spurious breeds by principal component analysis and hierarchical cluster analysis. Anofinic acid and its derivatives, as the characteristic markers, could be used for the identification and quality control of GMR.

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Secoiridoids from the roots of Gentiana straminea

Cited by 22 publications

References 17 publications

Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

Qualitative and quantitative determination of ten iridoids and secoiridoids in Gentiana straminea Maxim. by LC-UV-ESI-MS

Antidermatophyte Activity of the Gentiopicroside-Rich n-Butanol Fraction from <b><i>Gentiana siphonantha</i></b> Maxim. Root on a Guinea Pig Model of Dermatophytosis

Establishment and application of a new HPLC qualitative and quantitative assay for Gentiana Macrophyllae Radix based on characteristic constituents of anofinic acid and its derivatives

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