Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

Saini, Jitendra Kumar; Agrawal, Ruchi; Satlewal, Alok; Saini, Reetu; Gupta, Ravi P.; Mathur, Anshu S.; Tuli, Deepak K.

doi:10.1039/c5ra05792b

Cited by 39 publications

(24 citation statements)

References 36 publications

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“…A similar trend was shown when analyzing data obtained from fermentations of hydrolyzates obtained at 50 °C for 72 h (Table 6). Even if a little lower cellulose conversion and ethanol concentration in the fermented mixture were recorded, which was probably due to a partial deactivation of the cellulolytic enzymes exposed to a high temperature (50 °C) for extended periods Saini et al 2015). Furthermore, when using a 10% pretreated biomass loading, the fermentation was slower than with a 5% or 7.5% solids loading (Table 6, Fig.…”

Section: Pre-hydrolysis and Simultaneous Saccharification And Fermentmentioning

confidence: 98%

“…Environmentally friendly energy resources for alternative fuels have increased in the last decade, with the aim of progressively replacing fossil fuels and reducing the amount of greenhouse gases released into the atmosphere (Hoyer et al 2009;Menegol et al 2016;Ventorino et al 2017). Bioethanol, produced from lignocellulosic biomass, is a possible alternative to fossil fuels used in transportation (Saini et al 2015). Bioethanol can be produced from several agro-industrial residues, municipal solid waste, sawdust, and dedicated energy crops, such as miscanthus, switchgrass, black poplar (Populus nigra L.), and giant reed (Arundo donax L.) (Liguori et al 2016;Neves et al 2016;Ventorino et al 2016a).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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Arundo donax was used to investigate the effect of the enzyme and substrate concentrations on hydrolysis, pre-hydrolysis and simultaneous saccharification and fermentation (PSSF), in comparison to simultaneous saccharification and fermentation (SSF). Hydrolysis was performed at 37 and 50 °C. At the highest biomass (10%) and enzyme (69.6 FPU/g cellulose) loadings, the highest glucose concentration (32.4 g/L) was obtained (at 50 °C). SSF resulted in a cellulose conversion (91.9%) and an ethanol concentration (19.8 g/L) higher than what was obtained using PSSF at 37 °C (86.9% and 18.8 g/L, respectively) and PSSF at 50 °C (81.6% and 17.7 g/L, respectively). A positive correlation between the cellulase concentration, cellulose conversion, and ethanol content was observed. In PSSF, the increase in the solids loadings caused a reduction in the % cellulose conversion, but the ethanol concentration in PSSF and SSF increased. SSF appeared to be the most advantageous process for bioethanol production from A. donax.

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Section: Pre-hydrolysis and Simultaneous Saccharification And Fermentmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Aliberti¹,

Ventorino²,

Robertiello³

et al. 2017

BioResources

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“…In addition, some of these thermotolerant microorganisms can produce distinctive enzymes that function under relatively high temperature conditions [16][17][18]. Thermotolerant yeasts have been found and isolated from a number of countries [19][20][21][22][23][24][25][26][27][28]. Of these, K. marxianus is a haploid, homothallic, thermotolerant, and hemiascomycetous yeast [29,30].…”

Section: Introductionmentioning

confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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Thermotolerant ethanologenic yeasts receive attention as alternative bio-ethanol producers to traditionally used yeast, Saccharomyces cerevisiae. Their utilization is expected to provide several benefits for bio-ethanol production due to their characteristics and robustness. They have been isolated from a wide variety of environments in a number of ASEAN countries: Thailand, Vietnam, Laos, and Indonesia. One of these yeasts, Kluyveromyces marxianus has been investigated regarding characteristics. Some strains efficiently utilize xylose, which is a main component of the 2nd generation biomass. In addition, the genetic basis of K. marxianus has been revealed by genomic sequencing and is exploited for further improvement of the strains by thermal adaptation or gene engineering techniques. Moreover, the glucose repression of K. marxianus and its mechanisms has been investigated. Results suggest that K. marxianus is an alternative to S. cerevisiae in next-generation bio-ethanol production industry. Indeed, we have succeeded to apply K. marxianus for bio-ethanol production in a newly developed process, which combines high-temperature fermentation with simultaneous fermentation and distillation under low pressure. This chapter aims to provide valuable information on thermotolerant ethanologenic yeasts and their application, which may direct the economic bioproduction of ethanol and other useful materials in the future.Recently, thermotolerant microorganisms were found among mesophiles with optimum growth temperatures that are 5-10°C higher than those of the typical mesophilic strains Fuel Ethanol Production from Sugarcane 122 Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries… http://dx.doi.org/10.5772/intechopen.79144 123Recently, there have been several reports on ethanol production at high temperatures using P. kudriavzevii (formerly known as I. orientalis). Several P. kudriavzevii strains were reported to grow and produce high levels of ethanol at high temperatures. The strain DMKU 3-ET15 was isolated from traditional fermented pork sausage in Thailand by an enrichment technique in a medium supplemented with 4% ethanol at 40°C. The strain produced 78.6 g/L ethanol from 180 g/L glucose at 40°C [20]. The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].

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“…1 Apart from this, wet explosion (WEx) is another promising method for thermochemical pretreatment of LCB where, additional features of oxygen supplementation and explosive decompression have been incorporated to adjust with different biomass feedstocks and subsequent bio-catalytic and microbial processes. They have signicant potential to reduce our fossil fuel dependence.…”

Section: Introductionmentioning

confidence: 99%

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

et al. 2015

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Requirement of high enzyme dosage for lignocellulosic biomass hydrolysis is one of the challenges for the viability of the second generation bioethanol technology. Here, an optimal enzyme mixture was developed by partially replacing the cellulase proportion with accessory enzymes (b-glucosidase, xylanase, pectinase, laccase) and its hydrolytic performance was compared with different commercial counterparts for the saccharification of pretreated wheat straw (PWS) using a 250 kg per day continuous pilot plant. Maximum degree of synergism was observed with xylanase followed by pectinase, laccase, and bglucosidase. The statistically optimized enzyme mixture enhanced hydrolysis by 51.23% and 40.66% in 6 h and 24 h, respectively. This study elucidates that presence of even small amount of oligomers and cellobiose pose a strong inhibition for the enzymes. Therefore, development of an optimal enzyme formulation is a sustainable approach to reduce overall enzyme loading for biomass saccharification.

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Second generation bioethanol production at high gravity of pilot-scale pretreated wheat straw employing newly isolated thermotolerant yeast Kluyveromyces marxianus DBTIOC-35

Abstract: Application of thermotolerant yeast Kluyveromyces marxianus DBTIOC-35 in SSF decreases overall process time, and increases productivity and yield by allowing elimination of presaccharification step and use of high biomass concentration, respectively.

Cited by 39 publications

References 36 publications

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Effect of Cellulase, Substrate Concentrations, and Configuration Processes on Cellulosic Ethanol Production from Pretreated Arundo donax

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Improved saccharification of pilot-scale acid pretreated wheat straw by exploiting the synergistic behavior of lignocellulose degrading enzymes

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