Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing

Wang, Qiannan; Jin, Ying; Deng, Xiaoyuan; Liu, Hanping; Pang, Hongwen; Shi, Pengju; Zhan, Zhigang

doi:10.1016/j.biomaterials.2015.03.011

Cited by 50 publications

(42 citation statements)

References 41 publications

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“…Previous results demonstrated that GFP-tagged MSCs could be traced for 4 weeks after surgical treatment [36]. In consideration of the above-mentioned findings, we used this strategy in our previous work [30] and found that almost no GFP fluorescent signals could be detected at 14 days after transplantation. Although GFP labelling of transfected MSCs can be maintained for several months in culture in vitro , we were unsure what had occurred when these MSCs were applied in real animal models.…”

Section: Discussionmentioning

confidence: 99%

“…ADM scaffolds were obtained from 10-week-old male BALB/c mouse full-thickness skin [30]. The donor mice were injected intraperitoneally with 0.5% chloral hydrate.…”

Section: Methodsmentioning

confidence: 99%

“…Our previous studies demonstrated that no fluorescent signals were detected in healing tissue 14 days after MSC-ADM scaffolds implantation [30, 31]. We speculated as to whether the different labeling modes might affect the stability of GFP expression in MSCs during the progression of wound healing.…”

Section: Introductionmentioning

confidence: 99%

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Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

Wang

Liu

et al. 2017

Cell Physiol Biochem

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Background/Aims: Bone marrow Mesenchymal stem cells (MSCs) are promising for promoting cutaneous wound healing through reinforcing cellular processes. We evaluated the effect of GFP-tagged MSCs transplantation on skin regeneration in excisional wounds in mice. Methods: MSCs from GFP-labeled transgenic mice were co-cultured with acellular dermal matrix (ADM) scaffolds, and MSC-ADM scaffolds were transplanted into surgical skin wounds of BALB/c mice. After implantation, the survival and behavior of MSCs were examined by second harmonic generation and two-photon excitation fluorescence imaging, western blotting and DNA amplification and sequencing. Results: GFP-tagged MSCs were retained inside the regenerating skin until day 14 post-transplantation. Alpha-smooth muscle actin (α-SMA) and vimentin (VIM) were detected at 3, 5, 7, and 14 days post-transplantation by immunofluorescence double labeling. Although the GFP⁺/α-SMA⁺- and GFP⁺/VIM⁺-cell numbers decreased gradually with healing time, α-SMA⁺- and VIM⁺-cell numbers significantly increased, most of them were endogenous functional cells which were related to angiogenesis and collagen fiber structural remodeling. Conclusion: Therefore, in the initial stage of wound healing, transplanted MSCs differentiated into functional cells and played paracrine roles to recruit more endogenous cells for tissue remodeling. With the disappearance of exogenous cells, endogenous cells were responsible for the latter stage of cutaneous wound healing.

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Section: Discussionmentioning

confidence: 99%

“…ADM scaffolds were obtained from 10-week-old male BALB/c mouse full-thickness skin [30]. The donor mice were injected intraperitoneally with 0.5% chloral hydrate.…”

Section: Methodsmentioning

confidence: 99%

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Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

Wang

Liu

et al. 2017

Cell Physiol Biochem

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“…MSC-CM delivers osteoinductive growth factors and cytokines that modulate the behavior of endogenous cells contributing to the formation of new tissue. Furthermore, the use of MC allows us to avoid some of the limiting factors associated with the clinical application of stem cells, such as the risk of tumorigenesis and transmission of infectious diseases [80], immunological incompatibility, costs and waiting time for cell ex vivo expansion [80].…”

Section: Perspectivesmentioning

confidence: 99%

“…The mechanisms through which MSCs enhance the bone tissue repair process are complex, since they can participate in the three phases of bone healing: inflammation, proliferation and remodeling [80]. The in vivo identity and location of MSC have been difficult to establish.…”

Section: Mscs Mechanism Of Actionmentioning

confidence: 99%

Regenerative Medicine: A New Paradigm in Bone Regeneration

Chaparro¹,

Linero²

2016

Advanced Techniques in Bone Regeneration

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Bone defects are the cause of functional disability and the restoration of skeletal function remains an important challenge on orthopedics, neurosurgery and oral and maxillofacial surgery. Because of the limitations of the currently used techniques for the reconstruction of bone defects and the difficulties for the implementation of new therapeutic strategies, a new paradigm in the field of reconstructive surgery has arisen, leading to tissue engineering and regenerative medicine. Mesenchymal stem cells (MSC) have emerged as a promising alternative for the treatment of bone lesions. It was postulated that the therapeutic action was the result of proliferation and differentiation of MSCs, replacing injured tissue. However, recent studies have shown that MSCs secrete a number of trophic factors that have a strong effect during repair and tissue regeneration. This represents a shift from a paradigm centered on MSC proliferation and differentiation to a new paradigm in which the MSCs exert their beneficial effect by the secretion of paracrine factors that induce endogenous repair mechanisms. This chapter will bring together basic and clinical aspects, focused on novel findings on MSC paracrine effect and the development of new therapeutic strategies based on growth factors, cytokines and signaling molecules involved in bone regeneration.

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Biomimetic Structural Protein Based Magnetic Responsive Scaffold for Enhancing Bone Regeneration by Physical Stimulation on Intracellular Calcium Homeostasis

Liang,

Zou,

et al. 2023

Adv Healthcare Materials

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Bone matrix has distinct architecture and biochemistry which present a barrier on synthesizing bone‐mimetic regenerative scaffolds. To mimic the natural structures and components of bone, we prepared the biomimetic structural decellularized extracellular matrix (ECM)/regenerated silk fibroin (RSF) scaffolds incorporated with magnetic nanoparticles (MNP) using a facile synthetic methodology. The ECM/RSF/MNP scaffold is hierarchically organized and interconnected porous structure with silk fibroin twined on the collagen nanofibers, mimicking the bone minimum unit morphology. The scaffold demonstrated saturation magnetization due to the presence of MNP, along with good cytocompatibility. Moreover, the β‐sheet crystalline domain of RSF and the chelated magnetic nanoparticles could mimic the deposition of hydroxyapatite and enhance compressive modulus of the scaffold by approximately 20%. The results indicated that an external static magnetic field (SMF) with a magnetic responsive scaffold effectively promoted cell migration, osteogenic differentiation, neogenesis of endotheliocytes in vitro and new bone formation in a critical‐size femur defect rat model. RNA sequencing revealed that the molecular mechanisms underlying this osteogenic effect involve calsequestrin‐2‐mediated Ca2+ release from the endoplasmic reticulum to activate Ca2+/calmodulin/calmodulin‐dependent kinase II signaling axis. Collectively, bionic magnetic scaffolds with SMF stimulation provide a potent strategy for bone regeneration through internal structural cues, biochemical composition and external physical stimulation on intracellular Ca2+ homeostasis.This article is protected by copyright. All rights reserved

show abstract

Second-harmonic generation microscopy for assessment of mesenchymal stem cell-seeded acellular dermal matrix in wound-healing

Cited by 50 publications

References 41 publications

Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

Activities of MSCs Derived from Transgenic Mice Seeded on ADM Scaffolds in Wound Healing and Assessment by Advanced Optical Techniques

Regenerative Medicine: A New Paradigm in Bone Regeneration

Biomimetic Structural Protein Based Magnetic Responsive Scaffold for Enhancing Bone Regeneration by Physical Stimulation on Intracellular Calcium Homeostasis

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