Second harmonic generation signal from type I collagen fibers grown in vitro

Fuentes-Corona, Cindy Grethel; Licea-Rodríguez, Jacob; Younger, Rebecca; Rangel-Rojo, R.; Potma, Eric O.; Rocha‐Mendoza, Israel

doi:10.1364/boe.10.006449

Cited by 26 publications

(15 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The assembly the collagen hydrogel used as a surrounding matrix for the microspheres, reflects an unhindered in vitro assembly of collagen fibrils. [ 70 ] Resulting collagen fibers show a fibrillar morphology with parallel fibers (see surrounding collagen hydrogel network in Figure 3A,B). The collagen fiber assembly within the microspheres was influenced by the alginate gelling process.…”

Section: Resultsmentioning

confidence: 99%

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Lehnert

Sikorski

2021

Macromolecular Bioscience

View full text Add to dashboard Cite

Mimicking the complexity of natural tissue is a major challenge in the field of tissue engineering. Here, a facile 2‐step fabrication method to prepare 3D constructs with distinct regions of high cell concentrations and without the need for elaborate equipment is proposed. The initial incorporation of cells in a sacrificial alginate matrix allows the addition of other, cell relevant biopolymers, such as, collagen to form a spatially confined, interpenetrating network at the microscale. A layered structure at the macroscale can be achieved by incorporating these cell‐containing microspheres in thin collagen layers. Cells are locally released by de‐gelling the alginate matrix and their attachment to the collagen hydrogel layers has been studied. The use of the murine pre‐osteoblast cell line MC3T3‐E1 as an example cell line shows that the cells behave differently in their cell migration pattern based on the initial composition of the alginate microspheres.

show abstract

Section: Resultsmentioning

confidence: 99%

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Lehnert

Sikorski

2021

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…Further, dynamic techniques that can acquire such data over time can be applied to live cultures and tissue explants and thus capture complex spatiotemporal dynamics of matrix remodeling. Such techniques include second harmonic generation (SHG) that can also be used to examine collagen fiber alignment ( Fuentes-Corona et al, 2019 ).…”

Section: Models To Study Interactions With Extracellular Matrix and Tension/pressurementioning

confidence: 99%

Targeting Tumor-Stromal Interactions in Pancreatic Cancer: Impact of Collagens and Mechanical Traits

Maneshi

Mason

Dongre

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDAC) has one of the worst outcomes among cancers with a 5-years survival rate of below 10%. This is a result of late diagnosis and the lack of effective treatments. The tumor is characterized by a highly fibrotic stroma containing distinct cellular components, embedded within an extracellular matrix (ECM). This ECM-abundant tumor microenvironment (TME) in PDAC plays a pivotal role in tumor progression and resistance to treatment. Cancer-associated fibroblasts (CAFs), being a dominant cell type of the stroma, are in fact functionally heterogeneous populations of cells within the TME. Certain subtypes of CAFs are the main producer of the ECM components of the stroma, with the most abundant one being the collagen family of proteins. Collagens are large macromolecules that upon deposition into the ECM form supramolecular fibrillar structures which provide a mechanical framework to the TME. They not only bring structure to the tissue by being the main structural proteins but also contain binding domains that interact with surface receptors on the cancer cells. These interactions can induce various responses in the cancer cells and activate signaling pathways leading to epithelial-to-mesenchymal transition (EMT) and ultimately metastasis. In addition, collagens are one of the main contributors to building up mechanical forces in the tumor. These forces influence the signaling pathways that are involved in cell motility and tumor progression and affect tumor microstructure and tissue stiffness by exerting solid stress and interstitial fluid pressure on the cells. Taken together, the TME is subjected to various types of mechanical forces and interactions that affect tumor progression, metastasis, and drug response. In this review article, we aim to summarize and contextualize the recent knowledge of components of the PDAC stroma, especially the role of different collagens and mechanical traits on tumor progression. We furthermore discuss different experimental models available for studying tumor-stromal interactions and finally discuss potential therapeutic targets within the stroma.

show abstract

“…Polarization-sensitive (or polarization-resolved) second harmonic generation (SHG) (referred to as P-SHG hereafter) imaging is one of nonlinear optical (NLO) microscopy approaches to analyze the collagen structure in dried and wet samples. The structural information such as anisotropy value, fiber orientation, nonlinear susceptibility tensor, χ(2), and helical pitch angle of collagen molecules can all be extracted from the dependence of SHG intensity on the interacted linear polarization at varied angles (Stoller et al, 2002;Campbell et al, 2018;Fuentes-Corona et al, 2019). As the results of P-SHG imaging present structural information at the molecular scale, it has been used for distinguishing various molecular species (Chen et al, 2009) based on distinct χ(2) ratios, which serve as unique biomarkers for the detection of any modification or alteration in response to a pathological condition (Kumar et al, 2015;Tokarz et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy

et al. 2021

View full text Add to dashboard Cite

show abstract

Second harmonic generation signal from type I collagen fibers grown in vitro

Cited by 26 publications

References 33 publications

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Application of Temporary, Cell‐Containing Alginate Microcarriers to Facilitate the Fabrication of Spatially Defined Cell Pockets in 3D Collagen Hydrogels

Targeting Tumor-Stromal Interactions in Pancreatic Cancer: Impact of Collagens and Mechanical Traits

Label-Free Characterization of Collagen Crosslinking in Bone-Engineered Materials Using Nonlinear Optical Microscopy

Contact Info

Product

Resources

About