Second-intention repair in the horse and pony and management of exuberant granulation tissue

Wilmink, Jacintha M.; Weeren, P. R. van

doi:10.1016/j.cveq.2004.11.014

Cited by 55 publications

(68 citation statements)

References 31 publications

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“…Os animais do estudo de Theoret et al (2001) foram bem mais jovens do que os utilizados no presente estudo. Adicionalmente, a região corporal onde foi quantificado o fator de crescimento também pode ter influenciado, pois já foi comprovado que existe diferença no tempo máximo de cicatrização entre lesões cutâneas localizadas nos membros e no tronco, sendo mais lento nas primeiras (Wilmink & van Weeren 2005).…”

Section: Discussionunclassified

“…Entretanto, ao que parece essa redução na pele é lenta, já que no presente estudo os valores do TGF-β1 permaneceram significativamente altos em relação ao tempo zero (0,94±0,69pg/g), tanto no grupo tratado (24,29±12,67pg/g) como controle (36,31±16,59pg/g), porém não houve a formação de tecido de granulação exuberante. Essa formação de tecido está intimamente relacionada com a inflamação crônica da pele, o que perpetua a liberação de mediadores como o TGF-β, que por sua vez estimula de forma excessiva a fibroplasia, ocasionando a formação do tecido (Theoret & Wilmink 2013), o que contraditoriamente inibe a contração (Wilmink & van Weeren 2005). Na realidade, estudos in vitro demonstraram que baseado no modelo de expressão dos genes do TGF-β, o efeito desse fator de crescimento no processo de cicatrização cutânea é paradoxo.…”

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Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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ABSTRACT.-Souza M.V., Pinto J.O., Costa M.M., Santos E.C., Garcia S.L.R. & Oliveira L.L. 2014.[Quantification of growth factors in horse skin treated with platelet-rich plasma.] Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas. Pesquisa Veterinária Brasileira 34(6):599-612. Departamento de Veterinária, Universidade Federal de Viçosa, Campus Universitário s/n, Viçosa, MG 36570-000, Brazil. E-mail: msouza@ufv.br Platelet-rich plasma (PRP) is a product derived from total blood centrifugation, rich in bioactive factors, such as growth factors. Despite largely used in healing processes, there is a controversy whether the therapy is effective in promoting skin healing. The objective of this study was to quantify and compare the concentrations of the factors TGF-β1 and PDGF-BB in PRP, blood plasma and skin, at different phases of the healing process of skin treated or not with PRP. Seven healthy crossbred 16 to 17-year-old geldings (16.14±0.63) were used. Three quadrangular-shaped lesions (6.25cm 2 ) were surgically induced into the right and left gluteal regions of all animals. Twelve hours after induction of the wounds, 0.5mL of the PRP was administered in each of the four edges of the wound in one of the gluteal regions (Treated group = TG) randomly chosen. The contralateral region was used as control (CG). The wounds were submitted to daily cleaning with Milli-Q water, and samples were obtained with a 6mm diameter biopsy Punch. Six skin biopsies were obtained, the first carried out immediately after the production of the wound (T0), and the others 1 (T1), 2 (T2), 7 (T3), and 14 (T4) days after the lesion was induced. The sixth biopsy (T5) was obtained after complete healing of the skin, which occurred at about day 37 (36.85±7.45, CG; 38.85±6.46, TG). EDTA blood samples were also obtained, at all the times mentioned. Quantification of TGF-β1 and PDGF-BB growth factors on the skin, PRP, and blood plasma was carried out by the ELISA technique. Data were statistically analyzed by the t test, Pearson correlation and regression, at a significance level of 5%. No difference was found between the groups in the values of the two growth factors measured on the skin, at the different times. Also, no correlation was found between the amount of growth factors present in the skin and plasma. On the other hand, a positive correlation was observed between PRP and skin in the treated group, for the growth factors TGF-β1 (r=0.31) and PDGF-BB (r=0.38), as well as between both growth factors present in PRP (r=0.81). Considering the growth factor concentrations at T0, the highest skin values (p<0.05) of TGF-β1, in both groups, occurred at T3 and T5. Higher values (p<0.05) of PDGF-BB occurred at T4 (TG) and T5 (CG). No plasma changes occurred at the concentration of these factors in relation to T0, suggest-1 Recebido em 5 de outubro de 2013.Aceito para publicação em 5 de junho de 2014.

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Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

et al. 2014

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“…In fact, treating distal leg wounds (DLWs) and the ensuing exuberant granulation tissue remains one of the most frustrating clinical challenges [3] . There have been countless topical preparations and medications that have been applied to DLWs over the millennia and even in recent times, but few have actually been scientifi cally shown to possess signifi cant clinical effi cacy.…”

Section: Introductionmentioning

confidence: 99%

Equine wound healing: influence of low level laser therapy on an equine metacarpal wound healing model

Jann

Bartels

Ritchey³

et al. 2012

Photonics &Amp; Lasers in Medicine

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Objective: To evaluate the effects of low level laser therapy (LLLT) on healing of full thickness symmetrical skin wounds in horses. LLLT is a therapeutic modality using the application of light, usually a low power laser or light emitting diode in the power range of 1 mW to 12 W that, in practical terms, promotes tissue regeneration as well as reducing infl ammation and pain. Study design: Experimental study. Animals: Healthy horses (n = 8). Methods: Full thickness, 2.5 cm square skin wounds were created in the mid-metacarpal region on one leg of eight normal horses. LLLT was used on limbs assigned to the experimental group and limbs assigned to the control group were allowed to heal without treatment. LLLT was administered using a line generated optical scanner with a dual diode laser system (model EML; Erchonia Laser Healthcare, McKinney, TX, USA) at a wavelength of 635 nm and an energy output of 17 mW per diode. Wound size was measured for an 80-day period post operatively. Eighty days after surgery incisional biopsies were examined histologically. Results: Wounds treated with LLLT healed faster than the control wounds ( p = 0.01). Wounds treated with LLLT were completely epithelialized at day 80 after surgery. Control wounds were not epithelialized at postoperative day 80. Conclusion: LLLT increased the rate of wound healing. Schl ü sselw ö rter: Wunden; Wundheilung; Mittelfu ß bereich; Low-Level-Laser-Therapie (LLLT); Epithelisierung. Keywords

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“…Isso acontece por diversos fatores, tais como a escassez de pele, o baixo suprimento sanguíneo, a presença de grande comprometimento tecidual, o alto risco de contaminação e o atraso na cicatrização. Além disso, existe uma predisposição ao desenvolvimento de tecido de granulação exuberante e fibrose na região distal dos membros dos equinos, o que pode ocasionar o desenvolvimento de feridas crônicas de difícil cicatrização (WILMINK & WEEREN, 2005).…”

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Enxertos De Pele E Cicatrização Em Equinos

Pedroso¹,

Mendes²,

Coelho³

et al. 2017

Enci Bio

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RESUMOAs feridas de pele são muito comuns na clínica de equinos. Os ferimentos localizados em regiões distais dos membros dos equinos são considerados particularmente complicados no que se refere à reparação tecidual. O processo de cicatrização ou de reparo é uma tentativa do organismo restaurar a integridade anatômica ou funcional do tecido lesionado e, didaticamente, pode ser dividido em três fases: fase inflamatória, fase fibroblástica e fase de maturação e remodelamento. Existem vários fatores que afetam o processo de cicatrização na espécie equina. Alguns tratamentos, produtos e procedimentos têm demonstrado benefício nesse processo. Os enxertos de pele são uma boa alternativa, especialmente para feridas em membros distais. O seu uso pode melhorar o resultado final da cicatrização, tanto em relação ao tempo, quanto em relação aos resultados cosméticos e funcionais. PALAVRAS-CHAVE: cavalos, feridas, inflamação, lesão, pinch, punch EQUINE SKIN GRAFTS AND WOUND HEALING ABSTRACTSkin wounds are very common in the equine practice. Wounds located in distal limbs are considered particularly complicated in regard to tissue repair. The process of healing or repair is an body attempt to restore the anatomical or functional integrity of the injured tissue and can be divided into three phases: inflammatory phase, fibroblast phase and maturation and remodeling phase. There are several factors that affect the healing process in the equine species. Some treatments, products and procedures have shown benefit in this process. Skin grafts are a good alternative,

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Second-intention repair in the horse and pony and management of exuberant granulation tissue

Cited by 55 publications

References 31 publications

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Quantificação de fatores de crescimento na pele de equinos tratada com plasma rico em plaquetas

Equine wound healing: influence of low level laser therapy on an equine metacarpal wound healing model

Enxertos De Pele E Cicatrização Em Equinos

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