Second Prize: Sustained Release Varnish Containing Chlorhexidine for Prevention of Biofilm Formation on Urinary Catheter Surface: <i>In Vitro</i> Study

Shapur, Nandakishore K.; Duvdevani, Mordechai; Friedman, Michael; Zaks, Batia; Gati, Irit; Lavy, Eran; Katz, Ran; Landau, Ezekiel H.; Pode, Dov; Gofrit, Ofer N; Steinberg, Doron

doi:10.1089/end.2011.0140

Cited by 20 publications

(18 citation statements)

References 31 publications

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“…The catheters were coated with SRV prepared as previously described . Briefly, ethyl cellulose (5 g), polyethylene glycol (4 g), and chlorhexidine to a final concentration of 1% were dissolved in 100 mL of ethanol.…”

Section: Methodsmentioning

confidence: 99%

“…The varnish was prepared by dissolving the active ingredient and the polymers until a homogeneous preparation was achieved . The SRV was applied to the catheters by a brush as an outer coat, resulting in a uniform coat on the catheters as previously demonstrated . After application, the solvent (ethanol) was allowed to evaporate and the coat remained as a uniform cover of the catheter.…”

Section: Methodsmentioning

confidence: 99%

“…The above 3 samples representing the proximal, middle, and distal portions of each catheter were rinsed with PBS solution as described above to remove any debris and bacteria that were not associated with the biofilm. Each 1 of the 3 samples was placed in 4% formaldehyde solution for 10 min and then rinsed with distilled water . Each sample was immersed in 0.1% propidium iodide solution for 30 min in a dark room and washed.…”

Section: Methodsmentioning

confidence: 99%

“…Thus chlorhexidine potentially is an effective coating material to prevent catheter‐associated UTI. The development of sustained‐release varnish (SRV) of chlorhexidine for use on urinary catheters has been reported recently …”

mentioning

confidence: 99%

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Evaluation of Urinary Catheters Coated with Sustained‐Release Varnish of Chlorhexidine in Mitigating Biofilm Formation on Urinary Catheters in Dogs

Segev

Bankirer

Steinberg

et al. 2012

Veterinary Internal Medicne

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Background: Biofilm formation occurs commonly on urinary catheters. Objectives: To assess the efficacy of urinary catheters coated with sustained-release varnish of chlorhexidine in decreasing catheter-associated biofilm formation in dogs.Animals: Thirty client-owned dogs. Methods: Prospective study. Thirteen dogs were catheterized with urinary catheters coated with sustained-release varnish of chlorhexidine (study group), and 13 dogs were catheterized with an untreated urinary catheter (control group). Presence and intensity of biofilm formation on the urinary catheters were assessed and compared between the groups by evaluating colony-forming units (CFU) of biofilm bacteria, and semiquantitatively, using confocal laser scanning microscopy and electron microscopy.Results: None of the dogs experienced adverse effects associated with the presence of the urinary catheters. Median CFU count of biofilm bacteria at all portions of the urinary catheter was significantly (P < .001) lower in the study compared with the control group. The degree of biofilm formation on the urinary catheters, as evaluated by confocal laser scanning microscopy and electron microscopy, was significantly lower in the study compared with the control group. Electron microscopy examination identified crystals on some of the urinary catheters. The proportion of catheters on which crystals were observed was significantly lower on the distal part of the urinary catheter in the study group compared with the control group (16.7% versus 66.7%, respectively; P = .04).Conclusions and Clinical Importance: Chlorhexidine sustained-release varnish-coated urinary catheters effectively decrease urinary catheter-associated biofilm formation in dogs.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Urinary Catheters Coated with Sustained‐Release Varnish of Chlorhexidine in Mitigating Biofilm Formation on Urinary Catheters in Dogs

Segev

Bankirer

Steinberg

et al. 2012

Veterinary Internal Medicne

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“…Although silver and nitrofural functionalized catheters were shown in a multicenter randomized clinical trial to offer little or no clinical benefit, at least for short-term (14 days) catheterization, 24 catheters coated with a CHX varnish have been reported, [25][26][27] and the early laboratory and animal data are encouraging, 28 showing a reduction in biofilm formation with the CHX-coated devices.…”

mentioning

confidence: 99%

Functionalization of ethylene vinyl acetate with antimicrobial chlorhexidine hexametaphosphate nanoparticles

Wood¹,

Maddocks²,

Grady³

et al. 2014

IJN

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Ethylene vinyl acetate (EVA) is in widespread use as a polymeric biomaterial with diverse applications such as intravitreal devices, catheters, artificial organs, and mouthguards. Many biomaterials are inherently prone to bacterial colonization, as the human body is host to a vast array of microbes. This can lead to infection at the biomaterial’s site of implantation or application. In this study, EVA was coated with chlorhexidine (CHX) hexametaphosphate (HMP) nanoparticles (NPs) precipitated using two different reagent concentrations: CHX-HMP-5 (5 mM CHX and HMP) and CHX-HMP-0.5 (0.5 mM CHX and HMP). Data gathered using dynamic light scattering, transmission electron microscopy, and atomic force microscopy indicated that the NPs were polydisperse, ~40–80 nm in diameter, and aggregated in solution to form clusters of ~140–200 nm and some much larger aggregates of 4–5 μM. CHX-HMP-5 formed large deposits on the polymer surface discernible using scanning electron microscopy, whereas CHX-HMP-0.5 did not. Soluble CHX was released by CHX-HMP-5 NP-coated surfaces over the experimental period of 56 days. CHX-HMP-5 NPs prevented growth of methicillin-resistant Staphylococcus aureus when applied to the polymer surfaces, and also inhibited or prevented growth of Pseudomonas aeruginosa with greater efficacy when the NP suspension was not rinsed from the polymer surface, providing a greater NP coverage. This approach may provide a useful means to treat medical devices fabricated from EVA to render them resistant to colonization by pathogenic microorganisms.

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Antibiotic-Free Solutions for the Development of Biofilm Prevention Coatings

2022

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Stents and urinary catheters are commonly used medical devices, whose need is forecasted to grow considering not only the world population increase but also its aging and sedentary lifestyle.Independently of the great development on biomaterials and device design, infection represents still a major cause of failure of these devices, with undeniable humane and economical costs. Different antibiotic-based solutions have appeared in the market to try to address the matter. However, there is growing evidence on the impact of antibiotic-resistant microorganisms on urinary tract medical-devices infections, and respective outcomes. A broad number of alternatives have been proposed, however, given the wide variability of results for different strategies, there remains a tremendous need to validate their clinical significance, particularly assuring patient safety. Additionally, most of these strategies might be advantageous while in combination with current therapies, so further studies are needed.

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Second Prize: Sustained Release Varnish Containing Chlorhexidine for Prevention of Biofilm Formation on Urinary Catheter Surface: In Vitro Study

Cited by 20 publications

References 31 publications

Evaluation of Urinary Catheters Coated with Sustained‐Release Varnish of Chlorhexidine in Mitigating Biofilm Formation on Urinary Catheters in Dogs

Evaluation of Urinary Catheters Coated with Sustained‐Release Varnish of Chlorhexidine in Mitigating Biofilm Formation on Urinary Catheters in Dogs

Functionalization of ethylene vinyl acetate with antimicrobial chlorhexidine hexametaphosphate nanoparticles

Antibiotic-Free Solutions for the Development of Biofilm Prevention Coatings

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