Secondary genepool of Australian <i>Cajanus</i> species contains sources of resistance to <i>Helicoverpa armigera</i> (Hübner)

Vanambathina, Prameela; Henry, Robert J.; Rachaputi, Rao C. N.; Furtado, Agnelo

doi:10.1111/aab.12730

Cited by 3 publications

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“…Crop wild relatives are known to harbor agronomically important traits [ 4 , 5 ] which form an efficient and ecofriendly approach for pest management. The genus Cajanus consists of a single cultivated C. cajan (pigeonpea) with the rest being wild relatives in different gene pools [ 6 , 7 ]. C. platycarpus is a wild relative of pigeonpea from the tertiary gene pool that possesses a plethora of resistance traits [ 8 , 9 , 10 ], including towards the pod borer [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Deciphering of Pod Borer [Helicoverpa armigera (Hübner)] Resistance in Cajanus platycarpus (Benth.) Offers Novel Insights on the Reprogramming and Role of Flavonoid Biosynthesis Pathway

Tyagi

Shashank

Chaudhary

et al. 2022

Toxins

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Management of pod borer, Helicoverpa armigera in pigeonpea (Cajanus cajan L.), an important legume crop, has been a pertinent endeavor globally. As with other crops, wild relatives of pigeonpea are bestowed with various resistance traits that include the ability to deter the H. armigera. Understanding the molecular basis of pod borer resistance could provide useful leads for the management of this notorious herbivore. Earlier studies by our group in deciphering the resistance response to herbivory through multiomics approaches in the pigeonpea wild relative, Cajanus platycarpus, divulged the involvement of the flavonoid biosynthesis pathway, speculating an active chemical response of the wild relative to herbivory. The present study is a deeper understanding of the chemical basis of pod borer (H. armigera) resistance in, C. platycarpus, with focus on the flavonoid biosynthesis pathway. To substantiate, quantification of transcripts in H. armigera-challenged C. platycarpus (8 h, 24 h, 48 h, 96 h) showed dynamic upregulation (up to 11-fold) of pivotal pathway genes such as chalcone synthase, dihydroflavonol-4-reductase, flavonoid-3′5′-hydroxylase, flavonol synthase, leucoanthocyanidin reductase, and anthocyanidin synthase. Targeted LC-MS analyses demonstrated a concomitant increase (up to 4-fold) in naringenin, kaempferol, quercetin, delphinidin, cyanidin, epigallocatechin, and epicatechin-3-gallate. Interestingly, H. armigera diet overlaid with the over-produced flavonoids (100 ppm) showed deleterious effects on growth leading to a prolonged larval period demonstrating noteworthy coherence between over-accumulation of pathway transcripts/metabolites. The study depicts novel evidence for the directed metabolic reprogramming of the flavonoid biosynthesis pathway in the wild relative to pod borer; plant metabolic potential is worth exploiting for pest management.

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