Secondary Metabolite Accumulation Associates with Ecological Succession of Endophytic Fungi in <i>Cynomorium songaricum</i> Rupr.

Cui, Jin‐Long; Zhang, Yanyan; Vijayakumar, Vinod; Zhang, Gang; Wang, Mengliang; Wang, Junhong

doi:10.1021/acs.jafc.8b01737

Cited by 30 publications

(21 citation statements)

References 46 publications

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“…This study investigated the relationships between endophytic fungal and bacterial diversity and host secondary metabolites. Spearman correlation analysis showed that secondary metabolites of R. palmatum were positively correlated with endophytic fungal diversity and abundance, which is consistent with the results of Cui et al [34], who reported significant correlations between metabolites of Cynomorium songaricum and endophytic fungi, but the relationship between endophytic bacteria and host was ignored in their study. This preliminary conclusion suggests that endophytic fungi of R. palmatum were used as reservoirs of new bioactive secondary metabolites and also as potential substitutes for secondary metabolites of R. palmatum.…”

Section: Discussionsupporting

confidence: 90%

Analysis of Endophyte Diversity of Rheum palmatum from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite

et al. 2021

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Investigations of the differences in the metabolites of medicinal plants have typically focused on the effects of external environmental factors. However, little is known about the relationship between endophytes diversity and host metabolites. We used high-throughput sequencing methods to compare the endophyte diversity of Rheum palmatum from eight different production areas in Gansu Province of China and to analyze the association between those areas and five secondary metabolites (aloe-emodin, rhein, emodin, chrysophanol, and physcion). The results show that the diversity and OTUs (Operational taxonomic units) abundance of endophytic fungi and bacteria of R. palmatum differed according to production area. Spearman analysis showed that the five secondary metabolites of R. palmatum were positively correlated with the diversity and abundance of endophytic fungi. Comparing both space and environmental differences to determine influences on community structure, VPA analysis revealed that geographic factors explained more difference in community composition of fungal and bacterial endophytes than climate factors. PICRUSt and FUNGuild predictive analysis indicated that metabolites were the primary components of endophytic bacteria in all samples, while the function of endophytic fungi was composed of dominant trophic modes (saprotroph and pathotroph), and relative abundances were different. Our results help elucidate the correlation of plant–microbe interactions and offer pivotal information to reveal the role of endophytes in the production of R. palmatum and its important secondary metabolite.

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Section: Discussionsupporting

confidence: 90%

Analysis of Endophyte Diversity of Rheum palmatum from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite

et al. 2021

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“…13 Therefore, systematic revealing of all compounds in C. songaricum, especially the composition and variation of secondary metabolites, is one of the most anticipated results at present. 14 In recent years, by applying high-throughput sequencing technology, increasing studies have found that each plant contains an amazing number of endophytic fungi. 15 These fungi exist internally or reside in intercellular spaces of plant cells, being mutually beneficial to plants and not causing allergic reactions in terms of their pharmacological activity, 16 which are considered to be part of plant composition and function, and have a major impact on plant metabolism.…”

Section: ■ Introductionmentioning

confidence: 99%

Correlation in Chemical Metabolome and Endophytic Mycobiome in Cynomorium songaricum from Different Desert Locations in China

Cui

Gong

Vijayakumar

et al. 2019

J. Agric. Food Chem.

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Cynomorium songaricum Rupr. is a valuable food and medicinal plant with functions, such as an increase in sexual function, mainly attributed to its complex secondary metabolites. However, the effect of internal microbes on metabolite production in C. songaricum is still largely unclear. In this study, the relationship between endophytes and differential secondary metabolites in C. songaricum from seven major producing regions of China were explored based on established methods of metabolomics and high-throughput sequencing. The results showed that there were 13 different marker metabolites, seven shared fungal OTUs, and numerous unshared OTUs among C. songaricum distributed at different locations in China and identified significant correlations between metabolites and endophytic fungi. Our study revealed that endophytic fungi may be one possible factor that can affect the plant secondary metabolite composition.

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“…It indicated that the content of loganic acid was correlated with endophytic fungi, the content of gentiopicroside, swertiamarine and sweroside were correlated with endophytic bacteria in the G. officinalis and G. siphonantha . However, Chen et al [ 3 ] and Cui et al [ 28 ] reported that that metabolites content of Rheum palmatum and Cynomorium songaricum were only correlated with endophytic fungi. Endophyte play a important role on the accumulation of secondary metabolite in medicinal plants [ 3 ], while endophyte may have different effects on the accumulation of secondary metabolites in different plants.…”

Section: Discussionmentioning

confidence: 99%

Analysis of endophyte diversity of two Gentiana plants species and the association with secondary metabolite

et al. 2022

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Background The influence of external environmental factors on secondary metabolites of medicinal plants has always been studied. However, little is known about the relationships between endophytes and host metabolites, especially the relationship differences between different plant species. Thus, we used high-throughput sequencing methods to compare endophyte diversity from roots of two closely related species, Gentiana officinalis and G. siphonantha, from the same production area, and analyze the association with four secondary metabolites (Gentiopicroside, Loganic acid, Swertiamarine and Sweroside). Results The fungal and bacteria communities’ richness and diversity of G. siphonantha was higher than G. officinalis. Ascomycota and Proteobacteria were dominant fungal and bacterial phylum of the two closely related species. At the genus level, Tetracladium and Cadophora were dominant fungal genus in G. officinalis and G. siphonantha samples, respectively. While Pseudomonas was dominant bacterial genus in two closely related species, with relative abundances were 8.29 and 8.05%, respectively. Spearman analysis showed that the content of loganic acid was significantly positively correlated with endophytic fungi, the content of gentiopicroside, swertiamarine and sweroside were significantly positively correlated with endophytic bacteria in the two related species. PICRUSt and FUNGuild predictive analysis indicated that metabolism and saprotroph was primary function of endophytic bacteria and fungi in the two related species. Conclusion Our results will expand the knowledge on relationships of plant-microbe interactions and offer pivotal information to reveal the role of endophytes in the production of Gentiana plant and its important secondary metabolite.

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Secondary Metabolite Accumulation Associates with Ecological Succession of Endophytic Fungi in Cynomorium songaricum Rupr.

Cited by 30 publications

References 46 publications

Analysis of Endophyte Diversity of Rheum palmatum from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite

Analysis of Endophyte Diversity of Rheum palmatum from Different Production Areas in Gansu Province of China and the Association with Secondary Metabolite

Correlation in Chemical Metabolome and Endophytic Mycobiome in Cynomorium songaricum from Different Desert Locations in China

Analysis of endophyte diversity of two Gentiana plants species and the association with secondary metabolite

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