Secondary metabolite production and related biosynthetic genes expression in response to methyl jasmonate in Castilleja tenuiflora Benth. in vitro plants

Rubio-Rodríguez, Elizabeth; Vera‐Reyes, Ileana; Sepúlveda-García, Edgar; Ramos-Valdivia, Ana C.; Trejo‐Tapia, Gabriela

doi:10.1007/s11240-020-01975-3

Cited by 14 publications

(5 citation statements)

References 48 publications

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“…Besides, the expression of the CHS gene was significantly repressed in infected material from the tolerant genotype, while it remained unaffected in E00 and Q8 embryogenic cells. In contrast, PAL and CHS genes were upregulated when in-vitro derived shoots established from the VA5 genotype were confronted for 17 days with P. cinnamomi in dual cultures (Martínez et al, 2021 ) corroborating that the transcriptional level of genes coding for PAL and CHS enzymes is tissue-dependent (Rubio-Rodríguez et al, 2021 ). Both, PAL and CS genes were upregulated after infection of the elicited VA5 and E00 lines, respectively ( Supplementary Figure 1C ).…”

Section: Discussionmentioning

confidence: 92%

“…In our work, however, none of the genes studied was upregulated when the MeJA-primed lines were challenged with the oomycete as compared to infected controls ( Supplementary Figures 1B, C ). These results could be explained by a decline in the expression at the time that samples were collected (14 days after elicitation) as described in Milan et al ( 2017 ) or by the suppression of gene expression induced by some metabolites which are produced by the phenylpropanoid pathway, as described in Rubio-Rodríguez et al ( 2021 ). Uncorrelation between terpene metabolite production and the expression of their biosynthesis genes was reported in a MeJA-treated genotype of Norway spruce (Mageroy et al, 2020 ).…”

Section: Discussionmentioning

confidence: 93%

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Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

et al. 2022

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The dieback syndrome affecting Quercus ilex and other oak species impels the search for tolerant plant genotypes, as well as methods of plant immunization against such infections. Elicitation treatments can be an effective strategy to activate plant defense response and embryogenic lines represent a promising tool to generate new tolerant genotypes and also to study early markers involved in defense response. The aim of the presented work was to investigate changes in gene expression, and in hormonal and phenolic profiles induced in three holm oak embryogenic lines (ELs) elicited with methyl jasmonate (MeJA) before and after infection with the oomycete Phytophthora cinnamomi, which is the main biotic agent involved in this pathogenic process. The three ELs, derived from three genotypes, showed different basal profiles in all tested parameters, noting that the VA5 naïve genotype from a scape tree was characterized by a basal higher expression in NADPH-dependent cinnamyl alcohol dehydrogenase (CAD) and chalcone synthase (CHS) genes and also by higher caffeic acid content. Our work also identifies changes triggered by MeJA elicitation in holm oak embryogenic lines, such as increases in ABA and JA contents, as well as in levels of most of the determined phenolic compounds, especially in caffeic acid in Q8 and E00 ELs, but not in their biosynthesis genes. Irrespective of the EL, the response to oomycete infection in holm oak elicited plant material was characterized by a further increase in JA. Since JA and phenols have been described as a part of the Q. ilex defense response against P. cinnamomi, we propose that MeJA may act as an induced resistance (IR) stimulus and that in our embryogenic material induced both direct (detected prior to any challenge) and primed (detected after subsequent challenge) defense responses.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 93%

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

et al. 2022

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“…MeJA has previously been used as a potent elicitor of specialized metabolism in plants, including those producing iridoids (e.g. ( Wang et al., 2010 ; Cao et al., 2016 ; Piątczak et al., 2016 ; Matekalo et al., 2018 ; Rubio-Rodríguez et al., 2021 ; Pu et al., 2022 ). This elicitor promotes changes in the accumulation of specialized metabolites at the transcriptional level of genes involved in their biosynthesis.…”

Section: Resultsmentioning

confidence: 99%

“…The changes in the expression levels were the most pronounced for NrNEPS3 and transcription factors NrJAZ3 and NrMYC2. Similarly, in Castilleja tenuifora the increase in the expression of some iridoid biosynthesis-related genes was the most pronounced 24 h after the MeJA treatment (Rubio-Rodrıǵuez et al, 2021). The selection of TF for the co-expression analysis was based on our previous studies, where NrMYC2 and NrYABBY5 are recognized as the potential positive regulators of NL biosynthesis in N. rtanjensis (Anicǐćet al, 2020).…”

Section: Phyllogenetic Analysis Of Nrprise2 and Nnprisementioning

confidence: 99%

Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)

Aničić,

Matekalo,

Skorić

et al. 2024

Front. Plant Sci.

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Iridoids, a class of atypical monoterpenes, exhibit exceptional diversity within the Nepeta genus (subfam. Nepetoidae, fam. Lamiaceae).The majority of these plants produce iridoids of the unique stereochemistry, with nepetalactones (NLs) predominating; however, a few Nepeta species lack these compounds. By comparatively analyzing metabolomics, transcriptomics, gene co-expression, and phylogenetic data of the iridoid-producing N. rtanjensis Diklić & Milojević and iridoid-lacking N. nervosa Royle & Bentham, we presumed that one of the factors responsible for the absence of these compounds in N. nervosa is iridoid synthase (ISY). Two orthologues of ISY were mined from leaves transcriptome of N. rtanjensis (NrPRISE1 and NrPRISE2), while in N. nervosa only one (NnPRISE) was identified, and it was phylogenetically closer to the representatives of the Family 1 isoforms, designated as P5βRs. Organ-specific and MeJA-elicited profiling of iridoid content and co-expression analysis of IBG candidates, highlighted NrPRISE2 and NnPRISE as promising candidates for ISY orthologues, and their function was confirmed using in vitro assays with recombinant proteins, after heterologous expression of recombinant proteins in E. coli and their His-tag affinity purification. NrPRISE2 demonstrated ISY activity both in vitro and likely in planta, which was supported by the 3D modeling and molecular docking analysis, thus reclassification of NrPRISE2 to NrISY is accordingly recommended. NnPRISE also displays in vitro ISY-like activity, while its role under in vivo conditions was not here unambiguously confirmed. Most probably under in vivo conditions the NnPRISE lacks substrates to act upon, as a result of the loss of function of some of the upstream enzymes of the iridoid pathway. Our ongoing work is conducted towards re-establishing the biosynthesis of iridoids in N. nervosa.

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“…The substance basis of the medicinal value of C. tenuiflora is mainly terpenoid and phenolic substances. Studies showed that external application of MeJA promoted the synthesis of iridoid glycosides and phenolic compounds of C. tenuiflora , and also up-regulated the expression level of enzyme genes in its biosynthesis process [ 52 ]. In addition, Dong et al showed that MeJA had a positive effect on the release of volatile compounds from the aromatic plant lavender [ 53 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

Shi

Cui

Zhang

et al. 2023

CIMB

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Background: Methyl jasmonate has an important effect on the synthesis of plant secondary metabolites. Schizonepeta tenuifolia Briq. has a wide range of pharmacological effects and the secondary metabolites are dominated by monoterpenes (pulegone, menthone). Objective: It is essential to determine the changes in secondary metabolites in S. tenuifolia under methyl jasmonate treatment and to probe the molecular mechanism. This can improve the accumulation of secondary metabolites in the medicinal plant S. tenuifolia and enrich the information gene expression at different MeJA levels, which can help to elucidate the molecular mechanism of monoterpenoid synthesis in S. tenuifolia. Methods: In this study, we determined the changes in the content of monoterpenoids in S. tenuifolia under methyl jasmonate treatment. Meanwhile, we established a transcriptome database of S. tenuifolia under methyl jasmonate level using high-throughput sequencing. Results: A certain concentration of MeJA promoted the accumulation of monoterpenoids in S. tenuifolia. The transcriptome database of S. tenuifolia leaves under 0, 50, 100 and 250 μM MeJA treatment was established. We generated 88,373 unigenes with an N50 length of 2678 bp, of which 50,843 (57.53%) can be annotated in at least one database. Compared with the CK (0 μM) group, 12,557 (50 μM), 15,409 (100 μM) and 13,286 (250 μM) differentially expressed genes were identified. GO and KEGG enrichment analysis revealed that JA signal transduction and monoterpenoid synthesis were the two most significant enrichment pathways. The expression levels of related DEGs involved in JA signaling and monoterpenoid synthesis were significantly up-regulated by MeJA. In addition, our phenotypic and differentially expressed gene association analysis revealed that monoterpenoid biosynthesis in S. tenuifolia was more associated with genes involved in plant trichome branching, phytohormone signaling and transcriptional regulation. Conclusions: This study confirmed that methyl jasmonate significantly promoted monoterpenoid biosynthesis in S. tenuifolia. A large number of genes responding to methyl jasmonate were associated with JA signaling and monoterpenoid biosynthesis.

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Secondary metabolite production and related biosynthetic genes expression in response to methyl jasmonate in Castilleja tenuiflora Benth. in vitro plants

Cited by 14 publications

References 48 publications

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

Effect of Methyl Jasmonate in Gene Expression, and in Hormonal and Phenolic Profiles of Holm Oak Embryogenic Lines Before and After Infection With Phytophthora cinnamomi

Functional iridoid synthases from iridoid producing and non-producing Nepeta species (subfam. Nepetoidae, fam. Lamiaceae)

Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids in Schizonepeta tenuifolia Briq.

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