Secondary Metabolites Contents and Antioxidant Capacities of <i>Acmella</i><i> </i><i>Oleracea</i><i>e</i> Grown under Different Growing Systems

Abstract

Acmella oleraceae (L.) R.K. Jansen (Asteraceae) is a therapeutically important medicinal plant used in traditional systems of medicine. Present study was undertaken to compare the total phenolic contents (TPC), total flavonoid contents (TFC), total antioxidant capacity (TAC) and Thin Layer Chromatography (TLC) profiles of areal parts and callus extracts of A. oleraceae grown under different growing systems (field grown, hydroponically grown and callus culture. Callus established in Murasige and Skoog (MS) medium, areal parts of field grown and hydroponically grown plants were extracted in 80% methanol. TPC, TFC and TAC were carried out by using colorimetric Folin-Ciocalteu method, aluminum nitrate method and Ferric Reducing Antioxidant Power (FRAP) assay respectively. TLC profiles were developed using established protocol. The best callus growth and the highest mean callus weight were observed in leaf explants established in MS medium supplemented with 2 mg/L BA and 1 mg/L IBA. Comparatively higher TPC (11.45 ± 0.17), TFC (12.33 ± 0.92) and TAC (10.27 ± 0.28) were observed in hydroponically grown plants. Order of TPC, TFC and TAC were increased as callus<field grown plants< hydroponically grown plants. The presence of higher TPC, TFC and TAC in hydroponically grown plants opens a new window for use of hydroponic system for growing of A. oleraceae for better secondary metabolites content.

Reproductive toxicity of the hydroethanolic extract of the flowers of Acmella oleracea and spilanthol in zebrafish: In vivo and in silico evaluation

“…As spilanthol is an amphiphilic compound, 47 it can be extracted from plants using solvents that vary in polarity such as Hex 18 and MeOH:H 2 O (4:1, v/v). 48 Studies report that HPLC quantification with UV detection allows the identification of the content of spilanthol in different extracts, 24,49,50,51 as it was observed in this study. Spilanthol showed solubility in chloroform and it had its structure determined by NMR, according to the data compared in the literature.…”

Screening of Local and Introduced Varieties of <i>Pogostemon heyneanus</i> Benth. (Lamiaceae), for Superior Quality Physical, Chemical and Biological Parameters

“…These varietal specific spots could be used for authentication of these two varieties. Thin layer chromatographic profile have been successfully used for authentication of Acmella oleraceae [22], Munronia pinnata and Andrographis paniculata [23], for authentication of field grown and hydroponically grown Acmella oleracea [24] and Gyrinops walla [25]. Essential oil content of local variety was lower (0.4 %) than the introduced variety (2.4%).…”

EFFECT OF POTASSIUM NITRATE ON THE PRODUCTION OF RICININE BY Ricinus communis AND ITS INSECTICIDAL ACTIVITY AGAINST Spodoptera frugiperda

“…It has been demonstrated that the secondary metabolites of hydroponic grown plants of Datura and Taxus can be 'milked' using hydroponic conditions and permeabilization (Gontier et al, 2002). In plants of Acmella oleraceae L (Asteraceae) growing in hydroponics, Abeysinghe et al (2014) were able to increase the total phenolics and flavonoids contents by supplying more N to the nutrient solution. Waller and Henderson (1961) and Waller and Yang (1965), who studied the biosynthetic pathway of ricinine from organic and inorganic compounds labeled with N 15 , found that the relative efficiency of incorporation of labeled compounds followed a gradient: formamide glutamine aspartate > > > NH 4 NO 3 nicotinamide (Amida-N) > > nicotinitrile KNO 3 (nitrile-N) 1-methyinicotinamida > (Amide-N) 1-methyilnicotinonitrilo > (nitrile N).…”