Antibacterial activity; Allelopathy verbena is known to have antispasmodic, antipyretic, sedative, digestive, antimicrobial, local analgesic, and antioxidant activities [13,14]. Due to these properties, lemon verbena represents a huge market potential for herbal preparation and of essential oil extraction [15]. Although plant secondary metabolites are generally associated with plant defense against herbivores and pathogens, these unique compounds can be involved in a broad array of ecological functions [16]. Phenolic compounds are one of the largest groups of secondary metabolites, consisting of four main groups divided according to the number of phenol rings and the structural elements that bind those rings, including flavonoids, phenolic acids, tannins, stilbenes and lignans [17]. Indeed, some secondary metabolites such as benzoxazinoids are regarded as natural pesticides against pathogens, microorganisms, fungi, insects, and weeds [18]. The ubiquitous use of agrochemicals has resulted in negative impacts on human health and the environment. The use of the biological activity of plant extracts against certain pests of crops remains an environmental friendly alternative. Allelopathy is a phenomenon whereby secondary metabolites synthesized by microorganisms or plants may positively