Secondary Metabolites from Food-Derived Yeasts Inhibit Virulence of Candida albicans

Kunyeit, Lohith; Kurrey, Nawneet K.; Anu-Appaiah, K A; Rao, Reeta P.

doi:10.1128/mbio.01891-21

Cited by 10 publications

(8 citation statements)

References 46 publications

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“…In this study, we have observed that aromatic alcohols exhibit inhibitory effects on the growth of S. cerevisiae in high concentrations, reaching several grams per liter, as shown in Figure 1 . Under normal conditions, yeast are incapable of producing these alcohols in such significant quantities, with growth media typically accumulating only 0.01–0.02 g/L of phenylethanol, tyrosol, and tryptophol ( Zupan et al, 2013 ; Kunyeit et al, 2021 ). Meanwhile, overexpression of individual ARO genes can increase the concentration of tyrosol to approximately 0.05 g/L ( Bisquert et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Tyrosol induces multiple drug resistance in yeast Saccharomyces cerevisiae

et al. 2023

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In yeast, multiple (pleiotropic) drug resistance (MDR) transporters efflux xenobiotics from the cytoplasm to the environment. Additionally, upon the accumulation of xenobiotics in the cells, MDR genes are induced. At the same time, fungal cells can produce secondary metabolites with physico-chemical properties similar to MDR transporter substrates. Nitrogen limitation in yeast Saccharomyces cerevisiae leads to the accumulation of phenylethanol, tryptophol, and tyrosol, which are products of aromatic amino acid catabolism. In this study, we investigated whether these compounds could induce or inhibit MDR in yeast. Double deletion of PDR1 and PDR3 genes, which are transcription factors that upregulate the expression of PDR genes, reduced yeast resistance to high concentrations of tyrosol (4–6 g/L) but not to the other two tested aromatic alcohols. PDR5 gene, but not other tested MDR transporter genes (SNQ2, YOR1, PDR10, PDR15) contributed to yeast resistance to tyrosol. Tyrosol inhibited the efflux of rhodamine 6G (R6G), a substrate for MDR transporters. However, preincubating yeast cells with tyrosol induced MDR, as evidenced by increased Pdr5-GFP levels and reduced yeast ability to accumulate Nile red, another fluorescent MDR-transporter substrate. Moreover, tyrosol inhibited the cytostatic effect of clotrimazole, the azole antifungal. Our results demonstrate that a natural secondary metabolite can modulate yeast MDR. We speculate that intermediates of aromatic amino acid metabolites coordinate cell metabolism and defense mechanisms against xenobiotics.

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Section: Discussionmentioning

confidence: 99%

Tyrosol induces multiple drug resistance in yeast Saccharomyces cerevisiae

et al. 2023

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“…Studies have found that pretreatment of Lb21 lactic acid bacteria could not only increase the lifespan through increasing the resistance to pathogenic methicillin‐resistant Staphylococcus aureus , and Lb21‐mediated MRSA resistance mainly acted on the DBL‐1 ligand in the TGF‐β signaling pathway (Mørch et al, 2021). Kunyeit et al (2021) found that food‐derived beneficial yeasts could regulate and protect the C. elegans , make them less damaged by Candida albicans and prolong their lives, providing new ideas for the treatment of fungal infections (Kunyeit et al, 2021). ML1206, which has 99% similarity with the marine Planococcus maritimus , was screened as a potential marine probiotic acting for the infection of C. elegans by Vibrio anguillarum .…”

Section: Application Of C Elegans Model To Evaluate Nutrition Of Acti...mentioning

confidence: 99%

Application of Caenorhabditis elegans in the evaluation of food nutrition: A review

Xiao

et al. 2023

eFood

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At present, more and more people are suffering from various diseases and it is particularly important to find a suitable biological model to evaluate the prevention and treatment effects of various drugs and active ingredients on diseases. In all biological models, Caenorhabditis elegans is a kind of organism that is easy to observe and cultivate, has a clear genetic background and a high degree of signal pathway conservation with humans. A variety of active ingredients such as polyphenols, polysaccharides, polypeptides, and vitamins exist from natural products, which has extremely high physiological functions and medicinal values. It is highly reliable to evaluate the trophic and functional properties of the active ingredients from natural products in C. elegans. The functional properties of the active ingredients from natural products such as antiaging, anti-oxidation, regulating lipid metabolism, preventing and treating Alzheimer's disease in C. elegans were clarified in this review. At the same time, its possible pathways were summarized. The literature revealed that it had great effect to evaluate the nutrition and functionality of the active ingredients from natural products in C. elegans. Finally, the shortcomings in this field and suggestions for further improvement in this field have been emphasized.

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“…These genes include HSP150 and YGP1 , which regulate responses to stress and acidic pH tolerance; HSP26 and SSA4 , which regulate heat responses; and ARO9 and ARO8 , which are involved in the biosynthesis of aromatic alcohols, such as phenylethanol and tryptophol [ 41 , 42 ]. These aromatic alcohols can inhibit the virulence of C. albicans [ 43 ]. Moreover, S. boulardii has been reported to secrete medium-chain fatty acids, mainly capric acid, with bioactivity against C. albicans hyphae and biofilm formation [ 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

Anti-Cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Models

Yousif,

Wu,

Gonzales

et al. 2024

Pharmaceutics

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Despite the well-documented health benefits of the probiotic Saccharomyces, its application in oral health has not been comprehensively assessed. Dental caries is a transmissible disease initiated by acid production of cariogenic bacteria and yeast, such as Streptococcus mutans and Candida albicans, on tooth enamel and followed by subsequent enamel demineralization. Here, we investigated the effect of two Saccharomyces strains (Saccharomyces boulardii and Saccharomyces cerevisiae) on S. mutans–C. albicans cross-kingdom interactions using a cariogenic planktonic model. Viable cells, pH changes, and gene expression were measured. S. cerevisiae and S. boulardii inhibited the growth of C. albicans in dual- and multi-species conditions at 4, 6, and 20 h. Saccharomyces also inhibited C. albicans hyphal formation. Furthermore, Saccharomyces reduced the acidity of the culture medium, which usually plummeted below pH 5 when S. mutans and C. albicans were present in the model. The presence of Saccharomyces maintained the culture medium above 6 even after overnight incubation, demonstrating a protective potential against dental enamel demineralization. S. boulardii significantly down-regulated S. mutans atpD and eno gene expression. Overall, our results shed light on a new promising candidate, Saccharomyces, for dental caries prevention due to its potential to create a less cariogenic environment marked by a neutral pH and reduced growth of C. albicans.

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Secondary Metabolites from Food-Derived Yeasts Inhibit Virulence of Candida albicans

Abstract: The gut microbiome, primarily established by food, is complex and contributes to the health of the host. Molecular mechanisms that regulate microbial interactions and host health remain unclear.

Cited by 10 publications

References 46 publications

Tyrosol induces multiple drug resistance in yeast Saccharomyces cerevisiae

Tyrosol induces multiple drug resistance in yeast Saccharomyces cerevisiae

Application of Caenorhabditis elegans in the evaluation of food nutrition: A review

Anti-Cariogenic Effects of S. cerevisiae and S. boulardii in S. mutans–C. albicans Cross-Kingdom In Vitro Models

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