Secondary Metabolites from the Endophytic Fungus Stemphylium lycopersici and Their Antibacterial Activities

Xu, Zhao-Long; Zheng, Na; Cao, Shi-Man; Li, Shiting; Mo, Tu-Xiang; Qin, Yu-Yue; Li, Jun; Yang, Ronghua

doi:10.1007/s10600-020-03256-z

Cited by 6 publications

(5 citation statements)

References 15 publications

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“…Metabolite 9 is known from a static culture extract of Dematophora arcuata ( (Oppong et al 2009), as Rosellinia arcuata) and other fungi, including other species of Xylariaceae (Schmeda-Hirschmann et al 2005;Chinworrungsee et al 2011;Wu et al 2011;Xu et al 2020). After 1 h of high-dose treatment, the test compounds were washed-out, the cells supplemented with fresh medium and given 1 h of recovery time prior to fixation and staining for F-actin reorganization (g, h, i).…”

Section: Discussionmentioning

confidence: 99%

Studies on the secondary metabolism of Rosellinia and Dematophora strains (Xylariaceae) from Iran

et al. 2022

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The xylariaceous genus Dematophora has recently been resurrected and segregated from Rosellinia based on a molecular phylogeny and morphological characters. This was an important taxonomic change because Dematophora in the current sense contains several important pathogens, while Rosellinia is limited to mainly saprotrophic species that have an endophytic stage in their life cycle and may even have beneficial effects on the host plants. During our ongoing work on the functional biodiversity of the Xylariales, we have encountered new strains of rosellinoid Xylariaceae from Iran and have studied their mycelial cultures for secondary metabolites in an attempt to establish further chemotaxonomic affinities. In the process, we isolated and identified 13 compounds, of which rosellisteroid (1), the cichorine derivative 2, and the alkaloid 3 are new. Out of these, nine were tested for their antimicrobial affinities with cytochalasin E (6) exhibiting weak activity against Schizosaccharomyces pombe. The cytotoxicity of three cytochalasin derivatives was examined and their effects on the F-actin cytoskeletal organization studied by fluorescence microscopy using fluorescent phalloidin. Cytochalasin E (6) and Δ6,12-cytochalasin E (7) showed strong and irreversible action on actin, while cytochalasin K (8) exhibited weaker, reversible effects.

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Section: Discussionmentioning

confidence: 99%

Studies on the secondary metabolism of Rosellinia and Dematophora strains (Xylariaceae) from Iran

et al. 2022

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“…The high percentage of secreted effectors predicted in S . lycopersici relative to other necrotrophic fungi might be related to its host range, whether they establish a pathogenic or endophytic nonpathogenic interaction (Husna et al., 2022; Xu et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The genome of S. lycopersici is only half the size but encodes a higher percentage of such proteins (11.9%), so the total number of proteins in both organisms is similar (De Wit et al, 2012;Franco et al, 2015;Medina et al, 2018). lycopersici relative to other necrotrophic fungi might be related to its host range, whether they establish a pathogenic or endophytic nonpathogenic interaction (Husna et al, 2022;Xu et al, 2020). Lyu et al (2015) described the reorganization or rearrangement of the cell wall that occurs in plant-fungal interactions as being essential for infection.…”

Section: Putative Non-secreted Effectorsmentioning

confidence: 99%

Genome analysis of three isolates of Stemphylium lycopersici differ in their virulence and sporulation ability: Identification of effectors, pathogenesis and virulence factors

Medina,

Franco,

Bartel

et al. 2024

Plant Pathology

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Tomato grey leaf spot is a fungal disease that provokes losses in tomato yield. The aim of this work is to analyse genomic differences among three isolates of Stemphylium lycopersici that differ in virulence and sporulation. The bioinformatics analysis led us to predict the identity of putative effectors, pathogenesis and virulence factors. Like the genome of other necrotrophic pathogens, Stemphylium encodes a wide spectrum of effectors, including an ample and diverse array of carbohydrate‐degrading enzymes. Interestingly, the number of predicted effectors was unrelated to virulence. Low virulence appeared to be associated with the presence of several double‐stranded RNAs from viruses as well as cellular processes related to protein degradation, redox and detoxifying processes and monoterpenes production. This is the first identification of the potential effectors of tomato–S. lycopersici interaction; nonetheless functional studies should be done.

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“…The presence of the following fungi in black cherry wood may positively limit its expansion: D. macrostoma (Phoma macrostoma), P. laundoniae, F. avenaceum, and Stemphylium sp. These are endophytes but can also transform from opportunists into pathogens after contact under appropriate conditions with a suitable host [55][56][57][58]. The fungi of the genus Stemphylium, which also infect plants of the genus Prunus [59], and Fusarium sp., whose presence in black cherry wood has also been confirmed [26], are interesting.…”

Section: Discussionmentioning

confidence: 99%

Fungal Community Taxa Differ in Diversity and Number between Live and Dead Prunus serotina Ehrh. Wood in a Protected Forest within Its Secondary Range of Distribution

Baranowska,

Korzeniewicz,

Behnke-Borowczyk

et al. 2023

Forests

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Prunus serotina is an important invasive plant species in Poland. It was introduced to European forests, even in places currently protected by law, such as national parks. One major factor contributing to the spread of this species is the lack of infecting pathogens and incomplete knowledge about the relationship between the plant and microorganisms. This study aimed to identify fungal communities collected from freshly cut stumps of live and dead black cherry tree specimens growing in a protected forest. The main working hypotheses were as follows: (i) fungal communities inhabiting the wood of dead and living trees will differ in diversity and the number of taxa; and (ii) saprotrophic fungi will dominate in the wood of dead tree stumps. This study applied Illumina sequencing based on the amplification of the fungal ribosomal ITSI region. The average number of sequences (OTU) obtained from the analysis of dead tree wood was 101,758, while that of living trees was 94,150. These sequences belonged to 312 taxa, among which 254 were isolated from the wood of dead trees and 171 from that of living trees. Among the saprotrophs on dead trees, the following species were identified: Stereum rugosum, Ganoderma adspersum, G. applanatum, Peniophora cinerea, and Ascocoryne cylichnium. On the other hand, in the wood of living trees, Cytospora leucostoma and Botrytis cinerea were the most abundant saprotrophic species. The fungal communities inhabiting the wood of dead and living trees differed in the diversity and abundance of taxa, thus confirming our hypothesis. The results of our research conducted in a protected area indicate that black cherry wood can be naturally colonized by many pathogen species that can further limit its expansion.

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Secondary Metabolites from the Endophytic Fungus Stemphylium lycopersici and Their Antibacterial Activities

Cited by 6 publications

References 15 publications

Studies on the secondary metabolism of Rosellinia and Dematophora strains (Xylariaceae) from Iran

Studies on the secondary metabolism of Rosellinia and Dematophora strains (Xylariaceae) from Iran

Genome analysis of three isolates of Stemphylium lycopersici differ in their virulence and sporulation ability: Identification of effectors, pathogenesis and virulence factors

Fungal Community Taxa Differ in Diversity and Number between Live and Dead Prunus serotina Ehrh. Wood in a Protected Forest within Its Secondary Range of Distribution

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