Secondary metabolites isolated from Richardia brasiliensis Gomes (Rubiaceae)

Abstract

RESUMO: "Metabólitos secundários isolados de Richardia brasiliensis Gomes (Rubiaceae)".A família Rubiaceae compreende cerca de 637 gêneros e aproximadamente 10700 espécies, ocorrendo essencialmente nas regiões tropicais do Brasil. Richardia brasiliensis Gomes, popularmente conhecida por "poaia branca", é uma planta nativa da região sul do Brasil, utilizada na medicina popular como anti-emética e no tratamento de diabetes. Este trabalho reporta o isolamento e identifi cação estrutural de um fl avonóide glicosilado, um triterpeno, uma cumarina e dois derivados de ácido benzóico, objetivando contribuir para quimiotaxonomia do gênero Richardia. Através deste estudo foi possível isolar e identifi car os metabólitos isorametina-3-O-rutinosídeo, ácido oleanólico, a cumarina escopoletina e os ácidos p-hidroxibenzóico e m-metoxi-p-hidroxi-benzóico, todos isolados pela primeira vez no gênero, exceto o último, apresentando, portanto, relevante importância quimiotaxonômica para o mesmo. As estruturas foram identifi cadas com o uso de técnicas espectroscópicas de IV, RMN 1 H e 13 C uni e bidimensionais e comparação com dados da literatura.Unitermos: Rubiaceae, Richardia brasiliensis, isorametina-3-O-rutinosídeo, ácido oleanólico, escopoletina. ABSTRACT:The family Rubiaceae comprises around 637 genera and approximately 10,700 species, occurring essentially in tropical regions of Brazil. Richardia brasiliensis Gomes, known popularly as "poaia branca", is native to Brazil south region, used in folk medicine as anti-emetic and in the treatment of diabetes. This work reports the isolation and structural identifi cation of a fl avonoid glycoside, a triterpene, a coumarin and two benzoic acid derivatives, aiming at contributing to the chemotaxonomy of the genus Richardia, through a phytochemical study of Richardia brasiliensis. By means of this study the metabolites isorhamnetin-3-O-rutinoside, oleanolic acid, the coumarin scopoletin and p-hydroxy-benzoic and m-methoxy-p-hydroxybenzoic acids were isolated and identifi ed. All of them, but the latter, were isolated for the fi rst time in the genus, thereby presenting relevant chemotaxonomic importance to it. The structures were identifi ed using spectroscopic techniques such as IR, one and two-dimensional 1 H and 13 C NMR besides comparison with literature data.

Germination test and ornamental potential of different basil cultivars (Ocimum spp.)

“…The development of agronomic techniques aiming at maximizing medicinal, aromatic, flavouring, and ornamental species cultivation is a way of assuring raw material regular supply, controlling factors that could influence its phytochemical and pharmacological quality (LOURENZANI et al, 2004;PINTO et al, 2008;CHAGAS et al, 2011). However, studies aiming at maximizing the production of basil and other herbs are scarce.…”

Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae)

“…There are no reports regarding the total phenolis and total flavonoid content in Spermacoce species. The Rubiaceae family is known for the production of alkaloids, iridoids, and anthraquinones (YOUNG et al, 1996), and flavonoids have been isolated from species of this family (LOPES et al, 2004, CARDOSO et al, 2005PINTO et al, 2008). Noiarsa et al (2006) identified 18 substances from the aerial parts of Spermacoce laevis Roxb.…”

Phytochemical investigation and <i>in vitro</i> antimicrobial activity of <i>Richardia scabra</i>

“…The results are good agreement with R. grandiflora which revealed the presence of two phenolic compounds, mmethoxy-p-hydroxybenzoic acid, hydroxybenzoic acid, two steroids in the mixture (β-sitosterol and stigmasterol) and chlorophyll derivative (pheophytin a). The phytochemicals investigation performed with R. brasiliensis revealed it had different classes of metabolites like coumarins, flavonoids, triterpenes, sterols and phenolic acids (Pinto et al, 2008).…”