Secondary Metabolites of the Endophytic Fungus Lachnum abnorme from Ardisia cornudentata

Abstract

Abstract:Fractionation of an EtOAc-soluble fraction of the solid fermentate of an endophytic fungus, Lachnum abnorme Mont. BCRC 09F0006, derived from the endemic plant, Ardisia cornudentata Mez. (Myrsinaceae), resulted in the isolation of three new chromones, lachnochromonins D-F (1-3), one novel compound, lachabnormic acid (4), along with nine known compounds (5-13). Their structures were elucidated by spectroscopic analyses. Alternariol-3,9-dimethyl ether (6) was given the correct data as well as 2D spectral analyses for the first time. This is the first report of the isolation of one unprecedented compound 4 from Lachnum genus, while all known compounds were also found for the first time from Lachnum. The effects of some isolates (3, 4, 7-9, 10, and 13) on the inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages were also evaluated. Several compounds exhibited weak inhibitory activity on lipopolysaccharide (LPS)-stimulated NO production in RAW 264.7 macrophages.

Mollicellins O–R, Four New Depsidones Isolated from the Endophytic Fungus Chaetomium sp. Eef-10

“…Plant endophytes are regarded as a sort of particular-biotope microorganisms that reside in the tissues of living plants without causing any immediate obvious negative effects in their host plants [1,2]. Metabolites of endophytic fungi such as taxol, sanguinarine and gallic acid are known to possess various rare and novel scaffolds with significant biological activities, including anticancer, antimicrobial and antioxidant activities [3,4,5].…”

“…Metabolites of endophytic fungi such as taxol, sanguinarine and gallic acid are known to possess various rare and novel scaffolds with significant biological activities, including anticancer, antimicrobial and antioxidant activities [3,4,5]. Recently, a great deal of interest has been generated in discovery of remarkable pharmacological agents from endophytic fungi [1]. In the past decades, the Chaetomium genus hase been revealed to be a rich source of natural products, such as alkaloids [6,7,8,9], azaphilones [10,11,12,13,14], chaetoglobosins [15,16,17,18], chaetoindicins [19], diketopiperazines [20,21,22] and terpenoids [23].…”

Secondary Metabolites From Rice Culture of Aspergillus Sp. Isolated From Melaleuca Subulata (Cheel) Craven Leaves and Their Anticancer Activity

“…They gave physical and UV data typical to the pattern of coumarin compounds like alternariol [26]. 1 H NMR data of them (table 2) showed the presence of four meta-coupling doublets each integrated for one proton (δH 6.44 to 7.18) and (δH 6.46 to 7.37) respectively indicated the presence of two tetrasubstituted aromatic rings. In addition to the presence of singlet signals at δH ≈ 2.7 integrated for three protons gave evidence for the presence of methyl groups attached to an aromatic ring.…”

“…Plant endophytes are considered as a fungal or bacterial microorganism that spends whole or part of its life in the living tissues of the plant without causing apparent symptoms of disease [1,2]. Taxol, sanguinarine, and gallic acid are examples for secondary metabolites obtained from the endophytic fungi known to possess significant biological activities like anticancer, antimicrobial, and antioxidant activities [3][4][5].…”

Anti-leishmanial and Anti-inflammatory Agents from Endophytes: A Review

“…BCRC 09F0006, endophyte of Ardisia cornudentata Mez led to the isolation of four new compounds lachnochromonins D-F and lachabnormic acid, along with nine known compounds. Among them, lachnochromonin F (100), lachabnormic acid (101), alternariol-9-methyl ether (102), alternariol (103), pestalorionol (104), 1,3,6-trihydroxy-8-methylxanthone (105), and palmitic acid (106) inhibited the NO production in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages [107]. In a similar investigation, Liu et al [108] reported that among the eight new compounds (pestaloporinates A-G and 14-acetylhumulane) isolated from endophytic fungus Pestalotiopsis sp., from Melia azedarach Linn., pestaloporinate B (107) was potent with IC 50 value of 19.0 μM.…”