Secondary Metabolites Produced by Coculture of <i>Pleurotus ostreatus</i> SY10 and <i>Pleurotus eryngii</i> SY302

Ge, Xiaoxuan; Wang, Yu; Sun, Chunxiao; Zhang, Ziping; Song, Lin; Tan, Lei; Li, Dehai; Yang, Song; Yu, Ge

doi:10.1002/cbdv.202100832

Cited by 8 publications

(10 citation statements)

References 18 publications

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“…Several other liquid media were uncommonly used in fungal–fungal co-culture system and resulted in production of 21 new SMs ( 55 – 75 , Figure 3 ). Pleurotusins A ( 55 ) and B ( 56 ) along with five known terpenoids were produced by two edible fungi Pleurotus ostreatus SY10 and P. eryngii SY302 when co-cultivated in liquid medium consisting of glucose 10 g/L, KH 2 PO 4 1 g/L, MgSO 4 0.5 g/L, peptone 2 g/L, and 1 L sterilized water [ 45 , 46 ]. A new N -methoxypyridone analog ( 57 ) was not produced by the monoculture of the two endophytic strains Camporesia sambuci FT1061 and Epicoccum sorghinum FT1062, but was synthesized in the co-culture, which consisted of mannitol 20 g/L, sucrose 10 g/L, monosodium glutamate 5 g/L, KH 2 PO 4 0.5 g/L, MgSO 4 ·7 H 2 O 0.3 g/L, yeast extract 3 g/L, corn steep liquor 2 mL/L, and 1 L distilled water [ 47 ].…”

Section: Fungal–fungal Co-culturementioning

confidence: 99%

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

et al. 2023

Microorganisms

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Fungi are an important and prolific source of secondary metabolites (SMs) with diverse chemical structures and a wide array of biological properties. In the past two decades, however, the number of new fungal SMs by traditional monoculture method had been greatly decreasing. Fortunately, a growing number of studies have shown that co-culture strategy is an effective approach to awakening silent SM biosynthetic gene clusters (BGCs) in fungal strains to produce cryptic SMs. To enrich our knowledge of this approach and better exploit fungal biosynthetic potential for new drug discovery, this review comprehensively summarizes all fungal co-culture methods and their derived new SMs as well as bioactivities on the basis of an extensive literature search and data analysis. Future perspective on fungal co-culture study, as well as its interaction mechanism, is supplied.

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Section: Fungal–fungal Co-culturementioning

confidence: 99%

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

et al. 2023

Microorganisms

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“…However, compared to their natural habitat, certain activating factors are often lacking under traditional axenic conditions, resulting in some gene clusters remaining silent. , Several strategies have been applied to awaken silent genes in fungi, including genetic engineering (such as overexpression or knockout of the genes for transcription factors and global regulators), , one strain many compounds (OSMAC, mainly by altering the culture conditions), , and coculture. , Among these methods, the coculture strategy can effectively activate silent metabolic pathways by simulating natural microbial relationships, such as competition and antagonism, thus avoiding the need to manipulate the complex genome of mushrooms and reducing the workload for screening . This strategy has been well applied to explore the metabolic potential of mushrooms, leading to the discovery of many novel antimicrobial compounds, including postredienes A–F, , pleurotusin A, copsin, and 23 R -hydroxy-(20 Z ,24 R )-ergosta-4,6,8(14),20(22)-tetraen-3-one . Thus, conducting in-depth research on medicinal mushrooms through the coculture strategy will facilitate the discovery of their antimicrobial substances, which may provide a research foundation for the development of antimicrobial agents and alternatives to antibiotics in medicine and husbandry realms to address the gradually intensifying antibiotic resistance …”

Section: Introductionmentioning

confidence: 99%

“…16,17 Among these methods, the coculture strategy can effectively activate silent metabolic pathways by simulating natural microbial relationships, such as competition and antagonism, thus avoiding the need to manipulate the complex genome of mushrooms and reducing the workload for screening. 9 This strategy has been well applied to explore the metabolic potential of mushrooms, 16 leading to the discovery of many novel antimicrobial compounds, including postredienes A− F, 9,18 pleurotusin A, 19 copsin, 20 and 23R-hydroxy-(20Z,24R)ergosta-4,6,8 (14),20 (22)-tetraen-3-one. 21 Thus, conducting in-depth research on medicinal mushrooms through the coculture strategy will facilitate the discovery of their antimicrobial substances, which may provide a research foundation for the development of antimicrobial agents and Fermentation, Extraction, and Purification.…”

Section: ■ Introductionmentioning

confidence: 99%

Discovery of New Antimicrobial Metabolites in the Coculture of Medicinal Mushrooms

Ji,

Tan,

Shang

et al. 2024

J. Agric. Food Chem.

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Bioactivity screening revealed that the antifungal activities of EtOAc extracts from coculture broths of Trametes versicolor SY630 with either Vanderbylia robiniophila SY341 or Ganoderma gibbosum SY1001 were significantly improved compared to that of monocultures. Activity-guided isolation led to the discovery of five aromatic compounds (1−5) from the coculture broth of T. versicolor SY630 and V. robiniophila SY341 and two sphingolipids (6 and 7) from the coculture broth of T. versicolor SY630 and G. gibbosum SY1001. Tramevandins A−C (1−3) and 17-ene-1-deoxyPS ( 6) are new compounds, while 1-deoxyPS ( 7) is a new natural product. Notably, compound 2 represents a novel scaffold, wherein the highly modified p-terphenyl bears a benzyl substituent. The absolute configurations of those new compounds were elucidated by X-ray diffraction, ECD calculations, and analysis of physicochemical constants. Compounds 1, 2, and 5−7 exhibited different degrees of antimicrobial activity, and the antifungal activities of compounds 6 and 7 against Candida albicans and Cryptococcus neoformans are comparable to those of fluconazole, nystatin, and sphingosine, respectively. Transcriptome analysis, propidium iodide staining, ergosterol quantification, and feeding assays showed that the isolated sphingolipids can extensively downregulate the late biosynthetic pathway of ergosterol in C. albicans, representing a promising mechanism to combat antibiotic-resistant fungi.

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“…However, currently only about 14 terpenoids have been identified in P. ostreatus, 11 of them from liquid hyphae fermentation and three of them from the fruiting body (see Figure S1 in the Supporting Information). − These numbers are relatively low compared with other basidiomycete fungi. For example, at least 36 terpenoids have been isolated from the edible and medicinal basidiomycete fungi Hericium erinaceus. − Thus, activating silent genes and exploring the terpenoid diversity of P.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel Terpenoids from the Basidiomycete Pleurotus ostreatus through Genome Mining and Coculture Optimization

Wang

et al. 2023

J. Agric. Food Chem.

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In our previous work, postredienes A−C, three unusual linear sesterterpenes with high antifungal activities, were isolated from Pleurotus ostreatus SY10 when cocultured with Trametes robiniophila SY636. However, their titers were low, and exploration of newly biosynthesized trace analogues is required. Herein, genome mining analysis predicted that 17 gene clusters are involved in terpenoid biosynthesis in P. ostreatus. Thus, coculture conditions for strains SY10 and SY636 were optimized using a single-factor test and Box−Behnken design. As a result, the titers of postredienes A−C were increased by over 2.5-fold, reaching 1.28 to 8.40 mg/L. Moreover, five new terpenoids, named postredienes D−H (1−5), were successfully isolated. Compound 1 exhibited activities against the human pathogenic fungi Candida albicans and Cryptococcus neoformans comparable to those of amphotericin B. Compound 2 represents a novel sesterterpene with a five-membered ring at C-7. The absolute configurations of 1−5 were elucidated by making the methoxyphenylacetic acid esters and acetonide derivatives, combined with ECD and NMR calculation. Two potential gene clusters and relevant biosynthetic pathways for 1−5 were subsequently proposed based on real-time reverse transcriptionquantitative PCR (RT-qPCR) analysis. The current study provides new insights into the research of terpenoid biosynthesis genes in P. ostreatus and other basidiomycetes.

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Secondary Metabolites Produced by Coculture of Pleurotus ostreatus SY10 and Pleurotus eryngii SY302

Cited by 8 publications

References 18 publications

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

The Potential Use of Fungal Co-Culture Strategy for Discovery of New Secondary Metabolites

Discovery of New Antimicrobial Metabolites in the Coculture of Medicinal Mushrooms

Discovery of Novel Terpenoids from the Basidiomycete Pleurotus ostreatus through Genome Mining and Coculture Optimization

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