Secondary Metabolites Produced by Honey Bee-Associated Bacteria for Apiary Health: Potential Activity of Platynecine

Manici, L.; Saccà, Maria Ludovica; Lodesani, Marco

doi:10.1007/s00284-020-02153-6

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…Honey bees have become a concern in the medical world because they are a potential source of antimicrobials. Secondary metabolites produced by bacteria found in honey bees are a source of natural compounds [44].…”

Section: Discussionmentioning

confidence: 99%

In Silico and In Vitro Evaluation of the Antimicrobial Potential of Bacillus cereus Isolated from Apis dorsata Gut against Neisseria gonorrhoeae

et al. 2021

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Antimicrobial resistance is a major public health and development concern on a global scale. The increasing resistance of the pathogenic bacteria Neisseria gonorrhoeae to antibiotics necessitates efforts to identify potential alternative antibiotics from nature, including insects, which are already recognized as a source of natural antibiotics by the scientific community. This study aimed to determine the potential of components of gut-associated bacteria isolated from Apis dorsata, an Asian giant honeybee, as an antibacterial against N. gonorrhoeae by in vitro and in silico methods as an initial process in the stage of new drug discovery. The identified gut-associated bacteria of A. dorsata included Acinetobacter indicus and Bacillus cereus with 100% identity to referenced bacteria from GenBank. Cell-free culture supernatants (CFCS) of B. cereus had a very strong antibacterial activity against N. gonorrhoeae in an in vitro antibacterial testing. Meanwhile, molecular docking revealed that antimicrobial lipopeptides from B. cereus (surfactin, fengycin, and iturin A) had a comparable value of binding-free energy (BFE) with the target protein receptor for N. gonorrhoeae, namely penicillin-binding protein (PBP) 1 and PBP2 when compared with the ceftriaxone, cefixime, and doxycycline. The molecular dynamics simulation (MDS) study revealed that the surfactin remains stable at the active site of PBP2 despite the alteration of the H-bond and hydrophobic interactions. According to this finding, surfactin has the greatest antibacterial potential against PBP2 of N. gonorrhoeae.

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Section: Discussionmentioning

confidence: 99%

In Silico and In Vitro Evaluation of the Antimicrobial Potential of Bacillus cereus Isolated from Apis dorsata Gut against Neisseria gonorrhoeae

et al. 2021

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“…Lactic acid from L. johnsonii AJ5 induced the mite’s death when fed to bees. The mechanism implied in V. destructor ’s mortality remains unknown and needs further confirmations [ 206 , 207 ].…”

Section: Biocontrol and Ipm Strategies For V Destructor Managementmentioning

confidence: 99%

“…The main drawback of fungi and bacteria, regardless the lack of data about long-term effects, relies on low specificity of their toxins and complication for them to colonise and survive in the ecosystem of the hive [ 172 , 207 ]. In addition, from a phylogenetic perspective, the honeybee and the ecto-parasite are relatively close, making it harder to reach a single one.…”

Section: Biocontrol and Ipm Strategies For V Destructor Managementmentioning

confidence: 99%

Varroa destructor from the Laboratory to the Field: Control, Biocontrol and IPM Perspectives—A Review

Vilarem

Piou

Vogelweith³

et al. 2021

Insects

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Varroadestructor is a real challenger for beekeepers and scientists: fragile out of the hive, tenacious inside a bee colony. From all the research done on the topic, we have learned that a better understanding of this organism in its relationship with the bee but also for itself is necessary. Its biology relies mostly on semiochemicals for reproduction, nutrition, or orientation. Many treatments have been developed over the years based on hard or soft acaricides or even on biocontrol techniques. To date, no real sustainable solution exists to reduce the pressure of the mite without creating resistances or harming honeybees. Consequently, the development of alternative disruptive tools against the parasitic life cycle remains open. It requires the combination of both laboratory and field results through a holistic approach based on health biomarkers. Here, we advocate for a more integrative vision of V. destructor research, where in vitro and field studies are more systematically compared and compiled. Therefore, after a brief state-of-the-art about the mite’s life cycle, we discuss what has been done and what can be done from the laboratory to the field against V. destructor through an integrative approach.

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“…The mediation of extracellular bacterial metabolites in beneficial interactions with honey bees has been reported [20,21], but little is known on VOCs produced by bacterial populations inhabiting hives. The microbiota associated with honey bees has lately been the subject of an increasing number of studies, and the most frequently reported taxa include Lactobacillaceae, Bacillaceae, Acetobacteraceae, Bifidobacteriaceae, Gilliamella and Fructobacillus [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria

2021

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Beehives are populated by bacterial species with a protective role against honey bee pathogens thanks to the production of bioactive metabolites. These compounds are largely unexploited despite their high potential interest for pest management. This study evaluated the capability of bacterial species associated with honey bees to produce 2-heptanone, a volatile organic compound with anesthetic properties of the parasitic mite Varroa destructor. The production of this compound was quantified by SPME-GC-MS in a culture filtrate of nine bacterial strains isolated from the surface of honey bees, and the biosynthetic potential was evaluated in bacterial species associated with apiaries by searching for protein homologs putatively involved in its biosynthesis by using biocomputational tools. The findings pointed out that 2-heptanone was produced by Acetobacteraceae bacterium, Bacillus thuringiensis and Apilactobacillus kunkeei isolates in concentrations between 1.5 and 2.6 ng/mL and that its production was strain-specific. Putative methylketone synthase homologs were found in Bacillus, Gilliamella, Acetobacteraceae, Bartonella and Lactobacillaceae, and the protein sequence results were distributed in nine Sequence Similarity Network (SSN) clusters. These preliminary results support the hypothesis that 2-heptanone may act as a mediator of microbial relationships in hives and provide contributions to assess the role and biosynthetic potential of 2-heptanone in apiaries.

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Secondary Metabolites Produced by Honey Bee-Associated Bacteria for Apiary Health: Potential Activity of Platynecine

Cited by 5 publications

References 49 publications

In Silico and In Vitro Evaluation of the Antimicrobial Potential of Bacillus cereus Isolated from Apis dorsata Gut against Neisseria gonorrhoeae

In Silico and In Vitro Evaluation of the Antimicrobial Potential of Bacillus cereus Isolated from Apis dorsata Gut against Neisseria gonorrhoeae

Varroa destructor from the Laboratory to the Field: Control, Biocontrol and IPM Perspectives—A Review

Biosynthesis of 2-Heptanone, a Volatile Organic Compound with a Protective Role against Honey Bee Pathogens, by Hive Associated Bacteria

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