Secondary metabolites production through biotechnological intervention: A Review

Nasim, Sekh Abdul; Aslam, Junaid; Kapoor, Rohit; Khan, Saeed Ahmad

doi:10.9755/ejfa.v22i3.4886

Cited by 12 publications

(5 citation statements)

References 75 publications

(64 reference statements)

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“…Therefore, the solution to this problem might lie in the development of a reliable in vitro-based protocol. The development of such techniques offers many advantages such as independence from environmental factors such as climatic and soil conditions, absence of biological influences, as well as decreases in cost and increases in the production of secondary metabolites with the aid of automated cell growth control and regulation of metabolic processes [4].…”

Section: Asteriscus Graveolens (Forssk) Less Belonging ------------mentioning

confidence: 99%

Phytochemical composition and pharmacological assessment of callus and parent plant of Asteriscus graveolens (Forssk.) Less. from Algerian Sahara

Belhadi¹,

Ouafi²,

Bouguedoura³

2020

Trop. J. Pharm Res

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Purpose: To determine the phytochemical composition, acute toxicity, as well as analgesic and antiinflammatory properties of extracts of the aerial parts and callus cultures of Asteriscus graveolens.Methods: Different standard chemical tests were used for the screening of bioactive compounds in aqueous extracts of aerial parts of Asteriscus graveolens (PAE) and callus (CAE). High-performance liquid chromatography (HPLC) analysis was performed to identify the constituents of the glycosidic extract (GE). Acute oral toxicity test was carried out to ascertain the safety of the plant material. The analgesic and anti-inflammatory properties of the extracts were determined using acetic acid-induced writhing test and carrageenan-induced edema test, respectively.Results: Phytochemical analysis showed some differences in composition between the aerial parts and callus cultures. Results from HPLC analysis revealed the presence of 12 phenolic compounds in the GE of adult plants. In addition to being orally safe (no mortality), all extracts showed significant inhibition of acetic acid-induced chemical pain: PAE and GE produced 63 and 68 % reductions in pain, respectively, relative to 60.26 % pain reduction by aspirin. All extracts exerted potent anti-inflammatory effects, with PAE producing the highest effect (73 %) at a dose of 100 mg/mL.Conclusion: Asteriscus graveolens extracts possess potent pharmacological properties due to the presence of some phenolic compounds, especially flavonoids. These findings provide a scientific basis for the traditional uses of Asteriscus graveolens, and indicate that it is a potential source of antiinflammatory agents. Keywords: Acute toxicity, Anti-nociceptive, Anti-inflammatory, Aqueous extract, Callus, Flavonoids

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Section: Asteriscus Graveolens (Forssk) Less Belonging ------------mentioning

confidence: 99%

Phytochemical composition and pharmacological assessment of callus and parent plant of Asteriscus graveolens (Forssk.) Less. from Algerian Sahara

Belhadi¹,

Ouafi²,

Bouguedoura³

2020

Trop. J. Pharm Res

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“…In tissue culture techniques, those plants which are diffi cult to grow in nature or synthesising important plant product in low amount are also propagated. It's also become easier to explore the biosynthetic pathway of secondary metabolites via use of these techniques (Nasim et al 2010 ) .…”

Section: Plant Tissue Culturementioning

confidence: 99%

Contribution of Biotechnological Tools in the Enhancement of Secondary Metabolites in Selected Medicinal Climbers

Srivastava

Singh

Misra

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Plants by nature protect themselves by producing secondary metabolites which often have pronounced bioactivities. The presence of these metabolites is responsible for the active interaction of plants with their environment defending themselves against a variety of herbivores and pathogenic microorganisms as well as various kinds of abiotic stresses. Climber plants contain large number of useful secondary metabolites. Besides their pharmacological role, secondary metabolites also contain some other properties. Artabotrys hexapetalus , a climbing herb, secretes oil which is used in perfume industry. Capparis zeylanica is a thorny stout climbing shrub, used in the treatment of snake bite and to cure small pox, cholera, etc. Stem barks and roots of Tinospora cordifolia , a woody climber, are used in dysentery and diarrhoea. Toddalia asiatica , an evergreen climber, produced nitidine secondary metabolite which contains anti-HIV and antimalarial and anticancerous properties, but its production is not enough for commercial supply. Tissue culture technology has emerged as a supplementary branch to fulfi l demands for this valuable secondary metabolite. Various in vitro methods for enhancement of secondary metabolites are available such as hairy root culture, treatment of elicitors and use of precursors and introduction of any foreign gene via bacterial transformation.Current progresses have been made in the fi eld of molecular biology through the alteration in metabolic skeleton of plant secondary metabolism. With the use of various genes (involved in the synthesis of enzymes and their regulatory proteins), diverse pathways have been traced and being transformed. Antisense technology has been emerged as an additional alternative for enhancement of secondary metabolites. In Tylophora indica enhancement in kaempferol, an antioxidant compound was observed by using precursors like salicylic acid, ornithine, cinnamic acid, tyrosine and phenylalanine. The total dry weight of 2-hydroxy-4-methoxy benzaldehyde was enhanced in hairy root cultures of Decalepis hamiltonii . The present chapter provides an insight on the use of biotechnological techniques for the enhancement of secondary metabolites in medicinal climbers.

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“…Harvesting mostly few individuals in a given area, differences in quality among plants from different areas, negatively effected biodiversity and more possibility of contamination or inpurities are driving forces to investigate the most efficient and useful alternative methods for gathering the species at desired amount and quality. At this moment, in vitro approaches may serve as one of the most efficient and proper techniques in order to increase knowledge on plant's biology which may give important clues for mass production harvestable plant reserve in an acceptable quality for production of further extracts [6].…”

Section: Introductionmentioning

confidence: 99%

Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

Yamaner

Erdağ

2020

Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji

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In this study, A protocol to callus formation and adventitious shoot regeneration of Hypericum adenotrichum Spach via direct or indirect organogenesis has been described.Callus induction was carried out by using leaves which were collected from their native environment. The maximum callus induction frequency has been observed on MS medium containing 4 mgL -1 BA and 0.2 mgL -1 NAA. These calli were not induced to shoot regeneration. Shoot formation was obtained by transferring of the callus from only BA-containing MS medium to MS medium containing 0.5 mgL -1 KIN. Maximum shoot number was observed when the callus was transferred from callus induction medium containing 3 mgL -1 BA to medium containing 0.5 mgL -1 KIN. Direct shoot formation developed on leaf explants of Hypericum adenotrichum on MS medium containing KIN alone. The highest shoot number of per explant was observed on MS medium with 1 mgL -1 KIN.

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Secondary metabolites production through biotechnological intervention: A Review

Cited by 12 publications

References 75 publications

Phytochemical composition and pharmacological assessment of callus and parent plant of Asteriscus graveolens (Forssk.) Less. from Algerian Sahara

Phytochemical composition and pharmacological assessment of callus and parent plant of Asteriscus graveolens (Forssk.) Less. from Algerian Sahara

Contribution of Biotechnological Tools in the Enhancement of Secondary Metabolites in Selected Medicinal Climbers

Hypericum adenotrichum SPACH‘UN KALLUS İNDÜKSİYONU VE ADVENTİF SÜRGÜN REJENERASYONU

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