Secondary repair of alveolar clefts using human mesenchymal stem cells

Behnia, Hossein; Khojasteh, Arash; Soleimani, Masoud; Tehranchi, Azita; Khoshzaban, Ahad; Keshel, Saeed Hidari; Atashi, Reza

doi:10.1016/j.tripleo.2009.03.040

Cited by 106 publications

(71 citation statements)

References 17 publications

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“…PRP (5% or 10%)-supplemented media heat-inactivated (platelet concentration 79.6 x 10 4 /µL) provided similar results for adipogenic and osteogenic differentiation when compared to 10% FBS (92). Besides, PRP supplemented αMEM also provides osteogenenic, chondrogenic and adipogenic differentiation promoting an increase of cell culture (96)(97)(98)(99)(100)(101)(102). HPL and HS were used for MSC expansion for clinical application.…”

Section: Resultsmentioning

confidence: 97%

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

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Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results. Thus, the aim of this study was to conduct a systematic scoping review to analyze whether VBD are effective FBS substitutes for MSC ex vivo expansion. The search was performed in SciVerse Scopus TM , PubMed, Web of Science TM , BIREME, Cochrane library up to January 2016. The keywords were selected using MeSH and entry terms. Two independent reviewers scrutinized the records obtained considering specific inclusion criteria. The included studies were evaluated in accordance with a modified Arksey and O' Malley's framework. From 184 found studies, 90 were included. Bone marrow mesenchymal stem cells (BMMSC) were presented in most of these studies. Overall, VBD allowed for either, maintenance of MCS's fibroblast-like morphology, high proliferation, high colony-formation ability and maintenance of multipotency. Besides. MSC expanded in VBD supplements presented higher mitogen activity than FBS. VBD seems to be excellent xeno-free serum for ex vivo expansion of mesenchymal stem cells. However, an accentuated heterogeneity was observed between the carried out protocols for VBD isolation did not allowing for direct comparisons between the included studies.

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Section: Resultsmentioning

confidence: 97%

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

et al. 2017

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“…Por su capacidad de diferenciación a múltiples linajes, específicamente por su potencialidad osteogénica y sus propiedades inmunomoduladoras, antiinflamatorias y anti-apoptóticas, las células madre mesenquimales se han convertido en la principal herramienta de la terapia celular para el tratamiento regenerativo de enfermedades que afectan funcionalmente al tejido óseo (1,3,8,24,(55)(56)(57).…”

Section: Discussionunclassified

“…Por todo esto, las células madre mesenquimales son hoy en día el centro de múltiples estudios clínicos para ciertas entidades como infarto del miocardio, diabetes, alteraciones cerebrales y de médula espinal, enfermedades cardiovasculares, enfermedad de injerto contra huésped, enfermedad de Crohn (1,6) y otras que involucran el tejido óseo y cartilaginoso (8,12,(23)(24)(25).…”

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Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

2013

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Biomédica 2014;34:67-78 Ad-MSC en hidrogeles de plasma sanguíneo humano Contribución de los autores:Itali M. Linero: diseño de experimentos, obtención, cultivo y caracterización de las Ad-MSC, elaboración del hidrogel de plasma sanguíneo humano, escritura y revisión del manuscrito. Adriana Doncel: obtención, cultivo y caracterización de las Ad-MSC, y revisión del manuscrito. Orlando Chaparro: diseño de experimentos, escritura y revisión del manuscrito. Introducción. La utilización de las células madre mesenquimales en la práctica clínica ha aumentado considerablemente en la última década, ya que juegan un papel favorable en los procesos de reparación y regeneración tisular, siendo la principal herramienta de la terapia celular para el tratamiento de enfermedades que afectan funcionalmente el tejido óseo y cartilaginoso. Objetivo. Evaluar la proliferación y capacidad de diferenciación osteogénica in vitro de células madre mesenquimales derivadas de tejido adiposo humano en un hidrogel de plasma sanguíneo. Materiales y métodos. Se obtuvieron células madre mesenquimales a partir de explantes de tejido adiposo humano y se caracterizaron por citometría de flujo; se buscó demostrar su multipotencialidad por su capacidad de diferenciación osteogénica y adipogénica. Se evaluó la proliferación celular y la capacidad de diferenciación osteogénica de las células cultivadas en hidrogeles de plasma sanguíneo.Resultados. Las células madre mesenquimales derivadas de tejido adiposo cultivadas en el hidrogel de plasma sanguíneo humano mostraron un patrón de proliferación muy similar al de las células cultivadas en monocapa y, además, mantuvieron su capacidad de diferenciación hacia el linaje osteogénico. Conclusiones. El hidrogel de plasma sanguíneo humano es un soporte adecuado para que las células madre mesenquimales derivadas de tejido adiposo humano proliferen y se diferencien hacia el linaje osteogénico y constituye un vehículo adecuado para su administración en regeneración del tejido óseo.Palabras clave: células madre, hidrogeles, plasma, regeneración ósea.doi: http://dx.doi.org/10.7705/biomedica.v34i1.1465 Proliferation and osteogenic differentiation of mesenchymal stem cells in hydrogels of human blood plasmaIntroduction: The use of mesenchymal stem cells in clinical practice has increased considerably in the last decade because they play a supporting role in the processes of tissue repair and regeneration, becoming the main tool of cell therapy for the treatment of diseases functionally affecting bone and cartilage tissue. Objective: To evaluate in vitro the proliferative and osteogenic differentiation ability of mesenchymal stem cells derived from human adipose tissue in a blood plasma hydrogel. Materials and methods: Mesenchymal stem cells were obtained from human adipose tissue explants and characterized by flow cytometry. Their multipotentiality was demonstrated by their ability to differentiate to adipogenic and osteogenic lineages. Cell proliferation and osteogenic differentiation ability of the cells cultured...

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“…In other clinical study patients with unilateral alveolar cleft, treated with the composite scaffold of demineralized bone mineral and calcium sulphate (Osteoset) loaded with mesenchymal stem cells (MSCs). Computed tomograms showed regenerated bone after 4 months [41].…”

Section: Mesenchymal Stem Cells (Mscs)mentioning

confidence: 96%

“…There are many universities and government research institutes in Iran, such as Royan Institute [24][25][26], Amirkabir University [19,20,[27][28][29][30] , Pasteur Institute of Iran [31][32][33][34], University of Tehran [20,35], Tarbiat Modares University [20,[36][37][38][39], Shaheed Beheshti University of Medical Sciences [40,41,43,45], Tehran University of Medical Sciences [42,44], National Institute for Genetic Engineering [46], Islamic Azad University [47][48][49], Iran University of Science and Technology [50,51] and Iran Polymer and Petrochemical Institute [52,53] are conducting active research on the development of artificial organs based on tissue-engineering technologies.…”

Section: To Presentmentioning

confidence: 99%

Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds

Gharravi¹,

Orazizadeh²,

Hashemitabar³

et al. 2012

JBiSE

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Secondary repair of alveolar clefts using human mesenchymal stem cells

Cited by 106 publications

References 17 publications

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Venous Blood Derivatives as FBS-Substitutes for Mesenchymal Stem Cells: A Systematic Scoping Review

Proliferación y diferenciación osteogénica de células madre mesenquimales en hidrogeles de plasma sanguíneo humano

Status of tissue engineering and regenerative medicine in Iran and related advanced tools: Bioreactors and scaffolds

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