Secondary structure construction and molecular identification of rRNA 18S V4 region E23-5–E23-6 of parasitic lice of Hominidae

Zhang, Wanyu; Li, Haoruo; Zhao, Yongtao; Guan, Chenglin; Chai, Rong; Yang, Chenxi; Li, Hu

doi:10.1016/j.actatropica.2022.106772

Cited by 3 publications

(1 citation statement)

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“…Therefore, obtaining a more efficient and reliable way to identify and differentiate A. japonicus or larvae has become crucial for field diagnosis and epidemiological investigation, and achieving this goal is foreseeable only through the utilization of molecular approaches. Recently, molecular tools employing genetic markers from the nuclear and mitochondrial (mt) DNA offer a fast and sensitive approach to unveiling the genetic makeup and phylogenetic relationships of targeted species and have been widely used for species-specific identification and differentiation among various organisms, including Argulus ( 13 , 19 , 22–25 ). For example, nuclear genetic markers, including small ribosomal protein 18 (18S), OPC19, and OPH11, have been employed to explore the genetic diversity and species identification of Argulus parasites ( 6 , 19 , 26 , 27 ).…”

Section: Introductionmentioning

confidence: 99%

Complete Mitogenome sequencing of the fish louse Argulus japonicus (Crustacea: Branchiura): Comparative analyses and phylogenetic implications

Wang,

Hu,

Wang

et al. 2024

Front. Vet. Sci.

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The fish louse Argulus japonicus, a branchiuran crustacean of the Argulidae family, is attracting increasing attention because of its parasitic tendencies and significant health threats to global fish farming. The mitogenomes can yield a foundation for studying epidemiology, genetic diversity, and molecular ecology and therefore may be used to assist in the surveillance and control of A. japonicus. In this study, we sequenced and assembled the complete mitogenome of A. japonicus to shed light on its genetic and evolutionary blueprint. Our investigation indicated that the 15,045-bp circular genome of A. japonicus encodes 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), and 2 ribosomal RNAs (rRNAs) with significant AT and GC skews. Comparative genomics provided an evolutionary scenario for the genetic diversity of 13 PCGs: all were under purifying selection, with cox1 and nad6 having the lowest and highest evolutionary rates, respectively. Genome-wide phylogenetic trees established a close relationship between species of the families Argulidae (Arguloida) and Armilliferidae (Porocephalida) within Crustacea, and further, A. japonicus and Argulus americanus were determined to be more closely related to each other than to others within the family Argulidae. Single PCG-based phylogenies supported nad1 and nad6 as the best genetic markers for evolutionary and phylogenetic studies for branchiuran crustaceans due to their similar phylogenetic topologies with those of genome-based phylogenetic analyses. To sum up, these comprehensive mitogenomic data of A. japonicus and related species refine valuable marker resources and should contribute to molecular diagnostic methods, epidemiological investigations, and ecological studies of the fish ectoparasites in Crustacea.

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