For next generation wireless communication systems, high throughput, low latency, and large user accommodation are popular and important required characteristics. To achieve these requirements for next generation wireless communication systems, an in-band full-duplex (IBFD) communication system is one of the possible candidate technologies. However, to realize IBFD systems, there is an essential problem that there exists a large self-interference (SI) due to the simultaneous signal transmission and reception in the IBFD systems. Therefore, to implement the IBFD system, it is necessary to realize a series of effective SI cancellation processes. In this study, we implemented a prototype of SI cancellation processes with our designed antenna, analog circuit, and digital cancellation function using an adaptive filter. For system implementation, we introduce software-defined radio (SDR) devices in this study. By using SDR devices, which can be customized by users, the evaluations of complicated wireless access systems like IBFD can be realized easily. Besides the validation stage of system practicality, the system development can be more effective by using SDR devices. Therefore, we utilize SDR devices to implement the proposed IBFD system and conduct experiments to evaluate its performance. The results show that the SI cancellation effect can reach nearly 100 dB with 10−3 order bit error rate (BER) after signal demodulation. From the experiment results, it can be seen obviously that the implemented prototype can effectively cancel the large amount of SI and obtain satisfied digital demodulation results, which validates the effectiveness of the developed system.