Secretagogue-induced apocrine secretion in the Harderian gland of the rat

Gesase, Ainory Peter; Satoh, Yóichi; Ono, Kazuyuki

doi:10.1007/s004410050666

Cited by 21 publications

(15 citation statements)

References 28 publications

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“…Occasionally, however, CK 7 marks this level in all apocrine glandular cells of the impala. We have also not observed a thin stalk between the apical protrusion and rest of the cytoplasm as described by Gesase et al (1996) in the Harderian gland. The existence of both exocytosis and apocrine mechanisms has been described repeatedly, e.g., in the human axilla (Schaumburg-Lever and Lever 1975), in the human ceruminal glands (Main and Lim 1976), in apocrine glands of humans and dogs (Kazumasa et al 1984;Hashimoto et al 1989), and in scent glands of rodents (Smith and Hearn 1979).…”

Section: Discussionmentioning

confidence: 54%

Cutaneous glands of male and female impalas ( Aepyceros melampus ): seasonal activity changes and secretory mechanisms

Welsch

Dyk²,

Moss

et al. 1998

Cell and Tissue Research

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The cutaneous glands of the forehead and the metatarsus were studied by histological and histochemical methods and electron microscopy in adult male and female impalas in various seasons of the year. All glandular areas consist of apocrine and holocrine glands, which, however, occur in different proportions. Our findings in the apocrine gland cells suggest (1) the synthesis and exocytosis of a glycoproteinaceous secretory product stored in secretory granules, (2) typical apocrine secretion of the transformed apical cytoplasm, and (3) transepithelial fluid transport. The Golgi apparatus and apical membrane have binding sites for several lectins (PNA, HPA, RCA I, WGA). Cytokeratins 7, 14 and 19 are expressed at various intracellular localizations, suggesting an active role in the secretory mechanisms. The glands of the male forehead show marked seasonal changes in activity that are correlated with the main phases of the reproductive cycle, with the highest cellular activity occurring during the rut in April/May. The female forehead glands are only moderately developed and do not undergo seasonal changes. The metatarsal glands are of equal size in males and females and show no seasonal changes in activity. This study supports the hypothesis that (1) forehead glands in the male have a signaling role in the rut and (2) the metatarsal glands have a more general, probably social role maintaining and restoring contact between herd members.

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Section: Discussionmentioning

confidence: 54%

Cutaneous glands of male and female impalas ( Aepyceros melampus ): seasonal activity changes and secretory mechanisms

Welsch

Dyk²,

Moss

et al. 1998

Cell and Tissue Research

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“…It occurs in sweat glands (Schaumburg-Lever and Lever 1975;Kurosumi et al 1984), the mammary gland (Kurosumi et al 1968), the seminal vesicles of different species (Riva 1967;Aumüller 1979;Plöen 1980;Agrawal and Vanha-Perttula 1988), in certain lobes of the prostate of various species (Dahl and Tveter 1973;Nicander et al 1974;Aumüller 1979), the epididymis (Manin et al 1995), scent and lacrimal glands (Smith and Hearn 1979;Gesase et al 1996), and only exceptionally at other locations, e.g., the choroid plexus (Gudeman et al 1989).…”

Section: Discussionmentioning

confidence: 98%

“…1 Schematic diagram of the processing of thick chopper sections used for pre-embedding immunoelectron microscopy modes in a single secretory cell type (Gesase et al 1996). We have therefore studied the intracellular transport and release of 115 K protein and secretory transglutaminase in the rat coagulating gland, using a combination of light-and electron-microscopic immunohistochemistry and of highresolution scanning electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous apocrine and merocrine secretion in the rat coagulating gland

Groos

Wilhelm

Renneberg

et al. 1999

Cell and Tissue Research

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The coagulating gland of the rat synthesizes two prevalent secretory proteins (transglutaminase and 115 K) that are discharched in a different manner, one being secreted in an apocrine fashion (transglutaminase) and the other one in a merocrine way (115 K). Differences in the intra- cellular pathway and the release of either protein were studied using immunofluorescence on semithin sections, immunoelectron microscopy of preembedding-processed chopper sections and postembedding-processed ultrathin sections of rat coagulating gland. Immunohistochemical staining using an anti-transglutaminase antibody resulted in dense labeling of the cytoplasm of secretory cells and their apical blebs, whereas the cisternae of the rough endoplasmic reticulum and the Golgi apparatus were completely unlabeled. When, on the contrary, the anti-115 K antiserum was used, dense labeling of the cisternae of the rough endoplasmic reticulum, the Golgi apparatus, and the secretory granules was seen. Intraluminal secretion was also labeled, but the secretory blebs remained unlabeled. Our findings show that, in the coagulating gland of the male rat, the two secretory proteins studied are processed in parallel, but at completely different intracellular pathways. They are released via different extrusion mechanisms. Transglutaminase is synthesized outside the endoplasmic reticulum, reaches the apical cell pole by free flow in the cytoplasm, and is released via apocrine blebs, the membranes of which appear to be derived from the apical plasma membrane. The protein 115 K, on the other hand, follows the classic route, being synthesized within the cisternae of rough endoplasmic reticulum, subsequently glycosylated in the Golgi apparatus, and released in a merocrine fashion. The mutual exclusion of the two secretory pathways and the regulation of the alternative release mechanism are still unresolved issues.

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“…A limited amount of secretion also occurred by exocytosis. The release of secretory material not only by an apocrine mechanism, but also by exocytosis, is reported to occur in other epithelial tissues as well [Gesase et al 1996;Aumüller et al 1999; Gesease and Sa- Fig. 27.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Histochemical Observations on the Crural Gland-Spur Apparatus of the Echidna <i>(Tachyglossus aculeatus)</i> together with Comparative Observations on the Femoral Gland-Spur Apparatus of the Duckbilled Platypus <i>(Ornithorhyncus anatinus)</i>

Krause

2009

Cells Tissues Organs

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The echidna and platypus have a crural/femoral gland that is linked by a large duct to a canalized, keratinous spur located on the medial side of the ankle. The echidna crural gland, like the femoral gland of the platypus, exhibits cyclic activity, being prominent in both monotremes when they are sexually active. In the present study, we compared the structure and histochemistry of these glands. During the active phase, the secretory epithelium forming the respective glands of both species increased in height and became packed with secretory granules that differed markedly in structure. Secretory granules of the echidna crural gland were electron dense and characterized by cores or areas of increased electron density. Those of the platypus were initially electron dense, but then became less dense and coalesced into irregular complexes of secretory material. Large cytoplasmic blebs extended from epithelial cell apices and appeared to be shed into the lumen, resulting in an apocrine mode of secretion. Exocytosis was also observed. A similar form of release of secretory product was not observed in the echidna. Secretory granules of both species were periodic acid-Schiff positive and stained for protein, suggesting that much of the secretory product was glycoprotein. Myoepithelial cells enveloped the secretory tubules of the platypus femoral gland, whereas they were not observed surrounding tubules comprising the echidna crural gland. During the quiescent phase, the epithelial cells of both species lost their secretory granules and decreased in height. As a result, the secretory tubules became smaller, intralobular connective tissue increased and the glands decreased in overall size.

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Secretagogue-induced apocrine secretion in the Harderian gland of the rat

Cited by 21 publications

References 28 publications

Cutaneous glands of male and female impalas ( Aepyceros melampus ): seasonal activity changes and secretory mechanisms

Cutaneous glands of male and female impalas ( Aepyceros melampus ): seasonal activity changes and secretory mechanisms

Simultaneous apocrine and merocrine secretion in the rat coagulating gland

Morphological and Histochemical Observations on the Crural Gland-Spur Apparatus of the Echidna <i>(Tachyglossus aculeatus)</i> together with Comparative Observations on the Femoral Gland-Spur Apparatus of the Duckbilled Platypus <i>(Ornithorhyncus anatinus)</i>

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