Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation

Guan, Hua; Zheng, Huiyuan; Zhang, Jin; Xiang, Aoqi; Li, Yongxin; Zheng, Huadong; Xu, Li; Liu, Enqi; Yu, Qi

doi:10.3892/etm.2021.10069

Cited by 5 publications

(5 citation statements)

References 39 publications

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“…Secretory frizzled-related protein (SFRP) 4 is an extracellular antagonist of Wnt signalling and regulates adipogenesis [ 39 ]. Overexpression of recombinant active SFRP4 protein promotes brown adipocyte differentiation and induces CEBPα, UCP-1, PRDM16, PGC1α and GLUT4 expression in a dose-dependent manner [ 40 ]. However, SFRP4 is differentially expressed in the subcutaneous and visceral adipogenic differentiation of C57BL/6 mice and may play different roles [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery

Chen

Jiang

et al. 2022

Adipocyte

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Bariatric surgery (BS) is a dependable method for managing obesity and metabolic diseases, however, the regulatory processes of lipid metabolism are still not well elucidated. Differentially expressed genes (DEGs) were analysed through three transcriptomic datasets of GSE29409, GSE59034 and GSE72158 from the GEO database regarding subcutaneous adipose tissue (SAT) after BS, and 37 DEGs were identified. The weighted gene co-expression network analysis (WGCNA), last absolute shrinkage and selection operator (LASSO) logistic regression and support vector machine-recursive feature elimination (SVM-RFE) algorithms further screened four key genes involved in the regulation of STMN2, SFRP4, APOE and MXRA5. The GSE53376 dataset was used to further confirm the differential expression of SFRP4, APOE and MXRA5 in the postoperative period. GSEA analysis reveals activation of immune-related regulatory pathways after surgery. Finally, the silencing of MXRA5 was found by experimental methods to affect the expression of PPARγ and CEBPα during the differentiation of preadipocytes, as well as to affect the formation of lipid droplets. In conclusion, SAT immunoregulation was mobilized after BS, while MXRA5 was involved in the regulation of lipid metabolism.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery

Chen

Jiang

et al. 2022

Adipocyte

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“…qRT-PCRwas performed as previously described [ 14 , 20 ]. Briefly, total RNA was isolated from the aortas of the ApoE KO mice by using the TRIzol Plus (Invitrogen, Carlsbad, CA, USA), and a SuperScript® III First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA) was utilized to synthesize cDNA.…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, it can also bind with the FZ receptor and afect the direction of intracellular signal transduction [7]. As a member of the SFRPs family, since the discovery of SFRP4 [10], studies on its role in diabetes [11], obesity [12], and lipid metabolism [13][14][15][16] have continuously revealed its important roles, providing a new direction and strategy for the treatment of diabetes and lipid metabolism-related diseases. In addition, the latest research studies found that SFRP4 also plays an important role in the occurrence and development of cardiovascular diseases [17].…”

Section: Introductionmentioning

confidence: 99%

SFRP4 Reduces Atherosclerosis Plaque Formation in ApoE Deficient Mice

Guan

Liu

et al. 2023

Cardiology Research and Practice

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Secreted frizzled related protein 4 (SFRP4), a member of the SFRPs family, contributes to a significant function in metabolic and cardiovascular diseases. However, there is not enough evidence to prove the antiatherosclerosis effect of SFRP4 in ApoE knock-out (KO) mice. ApoE KO mice were fed a western diet and injected adenovirus (Ad)-SFRP4 through the tail vein for 12 weeks. Contrasted with the control cohort, the area of atherosclerotic plaque in ApoE KO mice overexpressing SFRP4 was reduced significantly. Plasma high-density lipoprotein cholesterol was elevated in the Ad-SFRP4 group. RNA sequence analysis indicated that there were 96 differentially expressed genes enriched in 10 signaling pathways in the mRNA profile of aortic atherosclerosis lesions. The analysis data also revealed the expression of a number of genes linked to metabolism, organism system, and human disease. In summary, our data demonstrates that SFRP4 could play an important role in improving atherosclerotic plaque formation in the aorta.

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“…Brown preadipocytes were isolated from the interscapular BAT of C57BL/6J mice aged 3 weeks using collagenase I digestion (Fig. 1B) [28]. The isolated cells were seeded in 6-well plates (1 × 10 6 cells/mL) and were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 1% penicillin/streptomycin and 10% fetal bovine serum.…”

Section: Isolation and Culture Of Brown Preadipocytesmentioning

confidence: 99%

CTRP9 Promotes Brown Adipose Tissue Lipolysis in Mice Fed a High-Fat Diet

Guan,

Wang,

Geng

et al. 2024

Front. Biosci. (Landmark Ed)

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Background: This study aimed to elucidate the molecular mechanism through which C1q/tumor necrosis factor (TNF)-related protein 9 (CTRP9) acts in the formation and differentiation of brown adipose tissue (BAT). Methods: Adenovirus particles encoding CTRP9 and green fluorescent protein were inoculated into the scapula of C57BL/6J mice and fed a high-fat diet for 8 weeks; the body weight, lipid droplet morphology, glucose tolerance, insulin tolerance, and protein expression levels were analyzed. In addition, CTRP9 adenovirus was transfected into brown preadipocytes, and differentiation was induced to identify the effect of CTRP9 overexpression on adipocyte differentiation. Results: CTRP9 overexpression significantly increased the weight gain of mice. Additionally, the CTRP9 overexpression group exhibited significantly increased adipose tissue weight and glucose clearance rates and decreased insulin sensitivity and serum triglyceride levels compared to the control group. Furthermore, CTRP9 overexpression significantly upregulated the adipose triglyceride lipase (ATGL) and perilipin 1 protein expression levels in BAT. The cell experiment results confirmed that CTRP9 overexpression significantly inhibited the adipogenesis of brown adipocytes as evidenced by the downregulation of uncoupling protein 1, beta-3 adrenergic receptor, ATGL, and hormone-sensitive lipase mRNA levels and the significant suppression of uncoupling protein 1, ATGL, and perilipin 1 protein levels in brown adipocytes. Conclusions: The finding of this study demonstrated that CTRP9 promotes lipolysis by upregulating ATGL expression in vivo and inhibits the differentiation of brown preadipocytes in vitro.

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Secreted frizzled‑related protein 4 promotes brown adipocyte differentiation

Cited by 5 publications

References 39 publications

Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery

Bioinformatics analysis to obtain critical genes regulated in subcutaneous adipose tissue after bariatric surgery

SFRP4 Reduces Atherosclerosis Plaque Formation in ApoE Deficient Mice

CTRP9 Promotes Brown Adipose Tissue Lipolysis in Mice Fed a High-Fat Diet

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