Secreted Glycoproteins That Regulate Synaptic Function: the Dispatchers in the Central Nervous System

Gong, Haiying; Zhu, Conglei; Han, Di; Liu, Sen

doi:10.1007/s12035-023-03731-y

Mol Neurobiol

2023

DOI: 10.1007/s12035-023-03731-y

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Secreted Glycoproteins That Regulate Synaptic Function: the Dispatchers in the Central Nervous System

Haiying Gong,

Conglei Zhu,

Di Han

et al.

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2024

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Unique Glycans in Synaptic Glycoproteins in Mouse Brain

Noel,

Suttapitugsakul,

Wei

et al. 2024

ACS Chem. Neurosci.

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The synapse is an essential connection between neuronal cells in which the membrane and secreted glycoproteins regulate neurotransmission. The post-translational modifications of glycoproteins with carbohydrates, although essential for their functions as well as their specific localization, are not well understood. Oddly, whereas galactose addition to glycoproteins is required for neuronal functions, galactosylation is severely restricted for Asn-linked on N-glycans in the brain, and genetic evidence highlights the important roles of galactose in brain functions and development. To explore this novel glycosylation, we exploited an orthogonal technology in which a biotinylated sialic acid derivative (CMP-biotin-Sia) is transferred to terminally galactosylated proteins by a recombinant sialyltransferase (rST6Gal1). This approach allowed us to identify the carrier proteins as well as their localization on brain sections. Immunohistochemical analysis of the biotinylated glycoproteins in brain sections demonstrates that they are largely positioned in the pre-and postsynaptic membranes. Consistent with this positioning, glycoproteomic analyses of the labeled glycoproteins identified a number of them that are involved in synaptic function, cell adhesion, and extracellular matrix interactions. The discovery of these galactosylated N-glycoproteins and their relative confinement to synapses provide novel insights into the unusual and specific nature of protein glycosylation in the brain.

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Unique Glycans in Synaptic Glycoproteins in Mouse Brain

Noel,

Suttapitugsakul,

Wei

et al. 2024

ACS Chem. Neurosci.

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Secreted Glycoproteins That Regulate Synaptic Function: the Dispatchers in the Central Nervous System

Cited by 1 publication

References 87 publications

Unique Glycans in Synaptic Glycoproteins in Mouse Brain

Unique Glycans in Synaptic Glycoproteins in Mouse Brain

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