Secreted miR-210-3p as non-invasive biomarker in clear cell renal cell carcinoma

Petrozza, Vincenzo; Pastore, Antonio Luigi; Palleschi, Giovanni; Tito, Claudia; Porta, Natale; Ricci, Serena; Marigliano, Chiara; Costantini, Manuela; Simone, Giuseppe; Carlo, Angelina Di; Gallucci, Michele; Carbone, Antonio; Fazi, Francesco

doi:10.18632/oncotarget.18449

Cited by 49 publications

(36 citation statements)

References 25 publications

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“…Thus, among molecules in exosomes, miRNAs have been investigated due to their regulatory role in gene expression [ 26 ]. Some reports have shown that oncogenic miRNA are associated with prognosis or as useful biomarkers in ccRCC [ 20 , 27 , 28 ]. Among oncogenic miRNAs, miR-224 is upregulated and plays an oncogenic function in hepatocellular carcinoma [ 27 , 29 , 30 ], esophageal squamous cell carcinoma [ 31 ], colorectal cancer [ 32 , 33 ], and non-small cell lung cancer [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our study is the first to show that exosomal miR-224 levels parallel those of miRNA-224 in ccRCC tissues and are significantly associated with prognosis in ccRCC patients. Interestingly Petrozza et al have reported that miR-210-3p extracted from urinary specimens in surgical treatment is useful as a non-invasive biomarker [ 28 ]. Moreover, expression of miR-210-3p extracted from urinary specimens in surgical treatment was associated with complete surgical resection or response to treatment in ccRCC [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly Petrozza et al have reported that miR-210-3p extracted from urinary specimens in surgical treatment is useful as a non-invasive biomarker [ 28 ]. Moreover, expression of miR-210-3p extracted from urinary specimens in surgical treatment was associated with complete surgical resection or response to treatment in ccRCC [ 28 ]. Another report showed miR-210 was potential biomarker for ccRCC diagnosis [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

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Extracellular miR-224 as a prognostic marker for clear cell renal cell carcinoma

et al. 2017

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Exosome-miRNAs (exo-miR) have recently been identified as modulators of cancer progression and distant metastasis. We previously found that intracellular miR-224 is up-regulated and significantly related to cancer invasion and metastasis in clear cell renal cell carcinoma (ccRCC). We therefore investigated the role of exosome miR-224 in ccRCC and explored the interaction between intra- and extracellular miR-224 in renal cell carcinoma. To validate the method for isolating exosomes from blood samples or cell culture media, we examined exosome morphology using transmission electron microscope (TEM). We investigated the relationship between exo-miR-224 expression and patient prognosis in 108 ccRCC patients. We isolated exosomes from a metastatic renal cancer cell line and tested their effects on a primary renal cancer cell line with several functional analyses. We found that the high expression level exo-miR-224 group has significantly shorter progression-free survival, cancer-specific survival, and overall survival compared with the low expression group. In multivariate analysis, a high level of exo-miR-224 was a significant risk factor related to all prognoses investigated. After adding exosomes from a metastatic RCC cell line to a primary RCC cell line, cell proliferation and invasion were increased while the percentage of apoptotic cells was significantly decreased. Intracellular levels of miR-224 were significantly up-regulated in the primary renal cancer cell line. Extracellular miR-224 in exosomes impacts on patient prognosis and is a potential prognostic biomarker for ccRCC patients.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Extracellular miR-224 as a prognostic marker for clear cell renal cell carcinoma

et al. 2017

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“…Hence, abnormal expression of miR‐210 can impact dramatically on cell behaviour, with a large body of evidence now gathered which links it with cancer development (Bavelloni et al, 2017; Dang & Myers, 2015). For example, overexpression of miR‐210 has been associated with breast (Camps et al, 2008; Li et al, 2013; Madhavan et al, 2012), renal (Petrozza et al, 2017), lung (He et al, 2018), colorectal (Sabry et al, 2018) and pancreatic (Greither et al, 2010) cancers among others. Together, these studies indicate a vital contributory role for miR‐210 in cancer development, as well as demonstrating its potential value as a prognostic cancer marker (Liu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

miR‐210 is induced by hypoxia and regulates neural cell adhesion molecule in prostate cells

Angel

Lynch

Nesbitt

et al. 2020

Journal Cellular Physiology

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Hypoxia in prostate tumours has been associated with disease progression and metastasis. MicroRNAs are short noncoding RNA molecules that are important in several cell processes, but their role in hypoxic signalling is still poorly understood.miR-210 has been linked with hypoxic mechanisms, but this relationship has been poorly characterised in prostate cancer. In this report, the link between hypoxia and miR-210 in prostate cancer cells is investigated. Polymerase chain reaction analysis demonstrates that miR-210 is induced by hypoxia in prostate cancer cells using in vitro cell models and an in vivo prostate tumour xenograft model. Analysis of The Cancer Genome Atlas prostate biopsy datasets shows that miR-210 is significantly correlated with Gleason grade and other clinical markers of prostate cancer progression. Neural cell adhesion molecule (NCAM) is identified as a target of miR-210, providing a biological mechanism whereby hypoxia-induced miR-210 expression can contribute to prostate cancer. This study provides evidence that miR-210 is an important regulator of cell response to hypoxic stress and proposes that its regulation of NCAM may play an important role in the pathogenesis of prostate cancer.

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“…Renal cell carcinoma (RCC) is the third most common urological malignancy in the USA and represents 2-3% of all adult malignancies. Furthermore, the incidence rate has progressively increased, particularly in young patients and or those with high-grade disease (1)(2)(3). In 2017, ~63,990 cases were diagnosed with kidney and renal pelvis cancer and 14,400 cases were expected to succumb to disease in the USA alone (4).…”

Section: Introductionmentioning

confidence: 99%

microRNA‑572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis

Pan

Zhao

et al. 2018

Oncol Rep

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Renal cell carcinoma (RCC) is the third most common urological malignancy in the USA and represents 2‑3% of all adult malignancies. Furthermore, the incidence of RCC has been progressively increasing over recent years. Although the morbidity of treatment has decreased with the use of multidisciplinary synthetic therapy, the prognosis of terminal cancer remains poor, with a 5‑year survival rate of 5‑10%. MicroRNAs (miRs) have been correlated with the regulation of 30‑60% of the protein-coding genes and act as oncogenes or anti‑oncogenes in RCC. Considering this research, miRNAs are likely to be the biomarkers for tumor diagnosis, prognosis and the targets for RCC management. In the present study, 42 formalin‑fixed paraffin‑embedded RCC samples were used. The expression of miR‑572 and the role of miR‑572 in RCC cell proliferation, migration and apoptosis was determined by performing reverse transcription‑quantitative polymerase chain reaction analysis, wound scratch assays, cell proliferation assays, Transwell assays and flow cytometry assays, respectively. Further experiments were conducted to clarify the correlation between miR‑572 expression and clinicopathological variables or overall survival. Furthermore, the expression levels of miR‑572 were evaluated for the prognosis value of patients with RCC. Upregulation of miR‑572 was observed in RCC tissues and RCC cell lines. miR‑572 promoted 786‑O and ACHN cell proliferation and mobility and inhibited early apoptosis. In Cox proportional hazard regression analyses, results of the univariate and multivariate analysis indicated that the patients with low miR‑572 expression had a significantly longer overall survival compared with the patients with high miR‑572 expression (univariate analysis, P=0.037; multivariate analysis, P=0.034). Results of the Kaplan‑Meier survival curves revealed that the patients with downregulated miR‑572 have a significantly longer overall survival compared with the patients with highly expressed miR‑572 (P=0.019). To conclude, the results of the present study suggest that tumor oncogene miR‑572 is a potential biomarker for the diagnosis, treatment and prognosis for RCC.

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Secreted miR-210-3p as non-invasive biomarker in clear cell renal cell carcinoma

Cited by 49 publications

References 25 publications

Extracellular miR-224 as a prognostic marker for clear cell renal cell carcinoma

Extracellular miR-224 as a prognostic marker for clear cell renal cell carcinoma

miR‐210 is induced by hypoxia and regulates neural cell adhesion molecule in prostate cells

microRNA‑572 functions as an oncogene and a potential biomarker for renal cell carcinoma prognosis

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