Secreted Peptide SpPIP1 Modulates Disease Resistance and Salt Tolerance in Tomato

Abstract

Tomato is a globally important horticultural and economic crop, but its productivity is severely affected by various stresses. Plant small secretory peptides have been identified as crucial mediators in plant resistance. Here, we conducted a comparative transcriptome analysis and identified the prePIP1 gene from Solanum pimpinellifolium (SpprePIP1), as an ortholog of Arabidopsis prePIP1 encoding the precursor protein of PAMP-induced SSP 1. The expression level of SpprePIP1 is transcriptionally induced in tomato upon infection with Phytophthora infestans (P. infestans), the pathogen responsible for late blight. Overexpression of SpprePIP1 resulted in enhanced tomato resistance to P. infestans. In addition, exogenous application of SpPIP1, whether through spraying or irrigation, improved tomato resistance by enhancing the transcript accumulations of pathogenesis-related proteins, as well as reactive oxygen species and the jasmonic acid (JA) levels. Integrated analysis of transcriptomics and metabolomics revealed the potential contributions of JA and phenylpropanoid biosynthesis to SpPIP1-induced tomato immunity. Additionally, SpPIP1 may strengthen tomato resistance to salt stress through the ABA signaling pathway. Overall, our findings demonstrate that SpPIP1 positively regulates tomato tolerance to P. infestans and salt stress, making it a potential plant elicitor for crop protection in an environmentally friendly way.

Regulatory Peptide Encoded by the Primary Transcript of miR396a Influences Gene Expression and Root Development in Solanum lycopersicum

“…Total RNA was extracted from S. lycopersicum leaves using RNAiso Plus (Code No. : 9108, Takara, Dalian, China) and RNA Extraction Buffer (Coolaber, Beijing, China) in accordance with the methods described by Yang et al 31 To ensure the elimination of genomic DNA during RNA reverse transcription, the PrimeScript RT reagent Kit (Takara, Dalian, China) with gDNA Eraser was utilized, following the provided protocol. For miRNA reverse transcription, the Mir-X miRNA First-Strand Synthesis Kit (TaKaRa, Dalian, China) was utilized, and the reaction system was as follows: mRQ Buffer (2x) 5 μL, RNA sample (0.25−8 μg) 3.75 μL, mRQ Enzyme 1.25 μL.…”

Research Progress of Small Plant Peptides on the Regulation of Plant Growth, Development, and Abiotic Stress

“…Recent findings indicate that ethylene downregulates the expression of CsRALF34 in cucumber [71], whereas in Arabidopsis, AtRALF34 is modulated by both auxin and ethylene [59]. Furthermore, the external application of SpPIP1 modulates jasmonic acid levels, thereby enhancing tomato resistance to stress [113]. GhRALF1 influences cotton's diurnal rhythms and fiber development through modulating auxin signaling and proton pump activity [114].…”

Constitutive expression of MsPROPEP1 enhances salinity and drought tolerance in alfalfa (Medicago sativa)

“…As yet, only scant evidence exists to support their contribution to a plant's tolerance to saltand drought stress other than Arabidopsis. Only SpPIP1 has been shown to be essential for the induction of salinity stress tolerance, as well as the immune response in tomato (Yang et al 2023). The exogenous application of synthesized broccoli BoPep4 peptide was shown to enhance tolerance to 200 mM NaCl in broccoli by reducing the Na + /K + ratio and by promoting the accumulation of wax and cutin in leaves (Wang et al 2022).…”