Secretion and formation of perimicrovillar membrane in the digestive system of the Sunn pest, Eurygaster integriceps (Hemiptera: Scutelleridae) in response to feeding

Abstract

In this study, development of perimicrovillar membrane (PMM) from midgut cells of starved and fed Eurygaster integriceps (Hemiptera: Scutelleridae) was studied. Three different approaches, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), marker enzymes of the PMMs (α-glucosidase), perimicrovillar space (aminopeptidase), and microvillar membranes (β-glucosidase) were used. Activities of these enzymes were remarkably low in the starved insects. Moreover, microscopic observations revealed that PMM is not present in the starved insect. Activities of enzymatic markers increased at 5 h postfeeding, and TEM and SEM observations showed the formation of PMM as well as migration of double-membrane vesicles from center of the columnar cell to the cell apex. The highest PMM was observed at 20 h postfeeding which at this time marker enzyme activity, such as α-glucosidase activity, was high, too. Thus, at 20 h postfeeding, PMM system was evident and epithelial cells were completely covered by PMM system. After 20 h postfeeding, presence of the fine holes in PMM started to be seen and at 40 h post-feeding, observation showed degradation of PMM system. Thus, it could be concluded that PMM in E. integriceps is secreted by epithelial cell membrane when needed and its secretion and formation is regulated by feeding. This system was not present in the starved insects as its development takes place at 5 h postfeeding.

Morphological and ultrastructural characterization of the alimentary canal in larvae of Streltzoviella insularis (Staudinger) (Lepidoptera: Cossidae)

“…The peritrophic membrane is also selectively permeable, and enhances digestion and absorption (Fonseca et al 2010;Mehrabadi & Bandani 2011;Zhong et al 2013).…”

Suppression of Bta11975, an α‐glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci

“…Mehrabadi et al . reported that AGLU activity was lower in starved than in non‐starved Eurygaster integriceps (Hemiptera: Scutelleridae) 56 . Healthy B. tabaci MED prefer ToCV‐infected tomato plants over non‐infected tomato plants 29 .…”

Origin, evolution and function of the hemipteran perimicrovillar membrane with emphasis on Reduviidae that transmit Chagas disease

“…In the hematophagous Cimex hemipterus (Hemiptera: Cimicidae), Triatoma pallidipennis and others triatomine species (Hemiptera: Reduviidae), of Cimicomorpha infraorder, the PMM is evident 20 and 15 days post feeding, respectively (Billingsley & Downe, 1986;Azevedo et al, 2009;Gutiérrez-Cabrera et al, 2014). In the phytophagous cotton stainer, D. peruvianus (Hemiptera: Pyrrhocoridae) and sunn pest, Eurygaster integriceps (Hemiptera: Scutelleridae), both of the Pentatomomorpha infraorder, the PMM covers all the cells 30 and 20 h post-feeding, respectively (Damasceno-Sá et al, 2007;Mehrabadi & Bandani, 2011). While in the zoophytophagous Brontocoris tabidus (Hemiptera: Pentatomidae), also of Pentatomomorpha infraorder, the PMM is evident in both the starved and fed condition (Fialho et al, 2009(Fialho et al, , 2013.…”