Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition

Fujimatsu, Teruhisa; Endo, Kiyoshi; Yazaki, Kazufumi; Sugiyama, Akifumi

doi:10.1002/pld3.259

Cited by 41 publications

(61 citation statements)

References 67 publications

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“…In addition to isoflavones, soybean roots secrete an equivalent amount of soyasaponins, which was first demonstrated by Tsuno et al (2018). Recently, soyasaponin Bb was shown to enrich Novosphingobium, commonly found in the soybean rhizosphere (Fujimatsu et al, 2020). The secretion of these specialized metabolites changes dramatically in quality and quantity depending on the developmental stage of the soybean plant (Sugiyama et al, 2016;Tsuno et al, 2018); however, the diurnal regulation of these soybean metabolites remains to be described.…”

Section: Introductionmentioning

confidence: 99%

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

et al. 2020

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“…In addition, also carotene (C40) which plays a role in photosynthesis (Fahy et al, 2005) and saponines which have been shown to modulate soil microbial communities (Fujimatsu et al, 2020), belong to this group. The roots of Cy.…”

Section: Discussionmentioning

confidence: 99%

“…It includes mono (C10) – and sequiterpenoids (C15), which are known for their roles in direct and indirect defence against herbivores and pathogens. In addition, also carotene (C40) which plays a role in photosynthesis (Fahy et al, 2005) and saponines which have been shown to modulate soil microbial communities (Fujimatsu et al, 2020), belong to this group. The roots of Cy.…”

Section: Discussionmentioning

confidence: 99%

Plot diversity differentially affects the chemical composition of leaves, roots and root exudates in four subtropical tree species

Weinhold

Döll

Liu

et al. 2020

Preprint

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Plants produce thousands of compounds, collectively called the metabolome, which mediate interactions with other organisms. The metabolome of an individual plant may change according to the number and nature of these interactions. We tested the hypothesis that tree diversity level affects the metabolome of four subtropical tree species in a biodiversity ecosystem-functioning experiment, BEF-China. We postulated that the chemical diversity of leaves, roots and root exudates increases with tree diversity. We expected the strength of this diversity effect to differ among leaf, root and root exudates samples. Considering their role in plant competition, we expected to find the strongest effects in root exudates.In an ecometabolomics approach, roots, root exudates and leaves of four tree species (Cinnamomum camphora, Cyclobalanopsis glauca, Daphniphyllum oldhamii, Schima superba) were sampled from selected plots in BEF-China. Samples were extracted and analysed using Liquid Chromatography-Time of Flight-Mass Spectrometry. The exudate metabolomes were normalized over their non-purgeable organic carbon level. Multivariate analyses were applied to identify the effect of both neighbouring (local) trees and plot diversity on tree metabolomes. The species and sample specific metabolites were assigned to major compound classes using the ClassyFire tool, whereas m/z features related to diversity effects were annotated manually.Individual tree species showed distinct leaf, root and root exudate metabolomes. The main compound class in leaves were the flavonoids, whereas carboxylic acids, prenol lipids and specific alkaloids were most prominent in root exudates and roots. Overall plot diversity had a stronger effect on metabolome profiles than the diversity of local, directly neighbouring trees. Leaf metabolomes responded more often to tree diversity level than exudates, whereas root metabolomes varied the least. We found not overall correlation between metabolite richness or diversity and tree diversity.Synthesis: Classification of metabolites supported initial ecological interpretation of differences among species and organs. Particularly the metabolomes of leaves and root exudates respond to differences in tree diversity. These responses were neither linear nor uniform and individual metabolites showed different dynamics. More controlled interaction experiments are needed to dissect the causes and consequences of the observed shifts in plant metabolomes.

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“…Recent studies have shown that saponins are secreted from plant roots into the rhizosphere [ 31 , 32 , 33 , 34 ]. Previously, we demonstrated that soyasaponins and α-tomatine were secreted from soybean and tomato roots in both hydroponic and field conditions [ 35 , 36 , 37 ]. Furthermore, both soyasaponins and α-tomatine were found to alter the bacterial communities and increase Sphingomonadaceae.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, both soyasaponins and α-tomatine were found to alter the bacterial communities and increase Sphingomonadaceae. Soyasaponins enriched Novosphingobium , whereas tomatine enriched Sphingobium at the genus level [ 35 , 37 ]. Based on these differential effects of the saponins in the soil bacterial communities, we hypothesized a link between saponin identity and soil bacterial community.…”

Section: Introductionmentioning

confidence: 99%

Triterpenoid and Steroidal Saponins Differentially Influence Soil Bacterial Genera

Nakayasu

Yamazaki

Aoki

et al. 2021

Plants

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Plant specialized metabolites (PSMs) are secreted into the rhizosphere, i.e., the soil zone surrounding the roots of plants. They are often involved in root-associated microbiome assembly, but the association between PSMs and microbiota is not well characterized. Saponins are a group of PSMs widely distributed in angiosperms. In this study, we compared the bacterial communities in field soils treated with the pure compounds of four different saponins. All saponin treatments decreased bacterial α-diversity and caused significant differences in β-diversity when compared with the control. The bacterial taxa depleted by saponin treatments were higher than the ones enriched; two families, Burkholderiaceae and Methylophilaceae, were enriched, while eighteen families were depleted with all saponin treatments. Sphingomonadaceae, which is abundant in the rhizosphere of saponin-producing plants (tomato and soybean), was enriched in soil treated with α-solanine, dioscin, and soyasaponins. α-Solanine and dioscin had a steroid-type aglycone that was found to specifically enrich Geobacteraceae, Lachnospiraceae, and Moraxellaceae, while soyasaponins and glycyrrhizin with an oleanane-type aglycone did not specifically enrich any of the bacterial families. At the bacterial genus level, the steroidal-type and oleanane-type saponins differentially influenced the soil bacterial taxa. Together, these results indicate that there is a relationship between the identities of saponins and their effects on soil bacterial communities.

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Secretion dynamics of soyasaponins in soybean roots and effects to modify the bacterial composition

Cited by 41 publications

References 67 publications

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

Diurnal metabolic regulation of isoflavones and soyasaponins in soybean roots

Plot diversity differentially affects the chemical composition of leaves, roots and root exudates in four subtropical tree species

Triterpenoid and Steroidal Saponins Differentially Influence Soil Bacterial Genera

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