Secretion of acid phosphatase from extraradical hyphae of the arbuscular mycorrhizal fungus Rhizophagus clarus is regulated in response to phosphate availability

Sato, Takumi; Hachiya, Shihomi; Inamura, Nozomi; Ezawa, Tatsuhiro; Cheng, Weiguo; Tawaraya, Keitaro

doi:10.1007/s00572-019-00923-0

Cited by 61 publications

(25 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The AM fungi can increase the plant sugar and leaf proline contents and maintain plant cells' osmotic potential, protecting the plant’s enzymatic activities. AM fungi also increased the acid phosphatase activity in roots, leading to the improved nutritional value of inoculated plants 20 . Moreover, AM fungi also increased Zn and P concentrations in plant tissues, thereby positively affecting plant physiological processes such as photosynthesis, respiration, and stomatal conductance.…”

Section: Introductionmentioning

confidence: 99%

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Saboor

Ali

Danish

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Zinc (Zn) deficiency can severely inhibit plant growth, yield, and enzymatic activities. Zn plays a vital role in various enzymatic activities in plants. Arbuscular mycorrhizal fungi (AMF) play a crucial role in improving the plant’s Zn nutrition and mitigating Zn stress effects on plants. The current study was conducted to compare the response of inoculated and non-inoculated maize (YH 1898) in the presence of different levels of zinc under greenhouse conditions under a Zn deficient condition. There were two mycorrhizal levels (i.e., M + with mycorrhizae, M- without mycorrhizae) and five Zn levels (i.e., 0, 1.5, 3, 6, and 12 mg kg-1), with three replicates following completely randomized design. At the vegetative stage (before tillering), biochemical, physiological, and agronomic attributes were measured. The results showed that maize plants previously inoculated with AMF had higher gaseous exchange traits, i.e., a higher stomatal conductance rate, favoring an increased photosynthetic rate. Improvement in antioxidant enzyme activity was also observed in inoculated compared to non-inoculated maize plants. Moreover, AMF inoculation also played a beneficial role in nutrients availability and its uptake by plants. Higher Zn12 (12 mg Zn kg-1 soil) treatment accumulated a higher Zn concentration in soil, root, and shoot in AMF-inoculated than in non-inoculated maize plants. These results are consistent with mycorrhizal symbiosis beneficial role for maize physiological functioning in Zn deficient soil conditions. Additionally, AMF inoculation mitigated the stress conditions and assisted nutrient uptake by maize.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Saboor

Ali

Danish

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…(2020). The beneficial impact of AMF on maize plant growth may be due to intensifying root growth, particularly fine roots with small radius (Abou El Seoud, 2008), developing depletion area nearby the plant rhizosphere depending on the hyphae of AMF (Abou El Seoud et al., 2020; Singh and Prabha, 2020), the mycorrhizal hyphae with fine radius can enter to the soil pore space tight to plant roots (Hammer et al., 2014), and transfer nutrients to the plant, as P (Rajtor & Piotrowska‐Seget, 2016; Smith, Jakobsen, Grønlund, & Smith, 2011), excreting organic compounds (Moe, 2013), increasing root exudation (Singh and Prabha, 2020), and phosphatase enzymes (Abdelhameed & Metwally, 2019; Chen et al., 2019; Sato et al., 2019). Also, AMF protect the photosynthesis process from the impact of HM toxicity (Yang et al., 2015).…”

Section: Resultsmentioning

confidence: 99%

Alleviation of cadmium and lead stress by A‐mycorrhizal fungi and rock phosphate on maize (Zea mays L.) growth under calcareous soil conditions

Seoud

Bakr²

2020

Environmental Quality Mgmt

View full text Add to dashboard Cite

This research was carried out to investigate the impact of A‐mycorrhizal fungi (AMF) and rock phosphate (RP) (alone and combined) under polluted soil with lead (Pb) and cadmium (Cd) on maize plant growth and soil nutrients availability under calcareous soil conditions. A greenhouse pot experiment was designed as a two‐factor experiment, remediation sources (RS) as the sub‐main factor with four treatments [control, M (mixed of the two mycorrhizae species, Rhizoglomus irregulare and Rhizoglomus macrocarpium), RP, and M + RP], and soil pollution sources (PS) as the main factor with nine treatments [control, Pb1 (100 mg Pb /kg soil), Pb2 (500 mg Pb/kg soil), Cd1 (6 mg Cd/kg soil), Cd2 (24 mg Cd/kg soil), Pb1 + Cd1, Pb1 + Cd2, Pb2 + Cd1, Pb2 + Cd2]. The results showed that, under the same conditions of this study, we can recommend the use of coapplication of AMF and RP (M + RP) particularly in contaminated soil with Pb and/or Cd, which supports plants to (i) confront the pollution stress, (ii) improve plants growth and their nutrients content, and (iii) increase the soil available nutrients. Also, maize plants could be labeled as the accumulator of Pb based on (translocation factor > 1) as control and under calcareous soil conditions.

show abstract

“…Over the past three decades, there have been extensive efforts to improve in vitro monoxenic culture using carrot hairy roots for AM fungi , with only P-free or 30 µM P as the low-P treatment (Bećard & Fortin, 1988b;Declerck et al, 1998;Douds, 2002;Karandashov et al, 2000;Olsson et al, 2002;Rosikiewicz et al, 2017). Our previous study showed that an extremely low concentration of P (3 µM) in the cocultivation plate using flax hairy roots increased AM fungal colonization (Sato et al, 2019), but the success rate of mycorrhizal formation, which is a prerequisite for spore production, remained to be assessed. This study screened critical factors for the success rate of mycorrhizal formation and found that the P concentration in the cocultivation plate is a critical factor for the mycorrhizal formation of several AM fungi.…”

Section: Discussionmentioning

confidence: 99%

Phosphorus is a critical factor of the in vitro monoxenic culture method for a wide range of arbuscular mycorrhizal fungi culture collections

Sato

Suzuki

Usui

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Establishing an effective way to propagate a wide range of arbuscular mycorrhizal (AM) fungi species is desirable for mycorrhizal research and agricultural applications. Although the success of mycorrhizal formation is required for spore production of AM fungi, the critical factors for its construction in the in vitro monoxenic culture protocol remain to be identified. In this study, we evaluated the growth of hairy roots from carrot, flax, and chicory, and investigated the effects of the phosphorus (P) concentration in the mother plate, as well as the levels of P, sucrose, and macronutrients in a cocultivation plate with a hairy root, amount of medium of the cocultivation plate, and location of spore inoculation, by utilizing the Bayesian information criterion model selection with greater than 800 units of data. We found that the flax hairy root was suitable for in vitro monoxenic culture, and that the concentration of P in the cocultivation plate was a critical factor for mycorrhizal formation. We showed that an extremely low concentration of P (3 μM) significantly improved mycorrhizal formation for AM fungi belonging to the Glomerales order, while a high concentration of P (30 μM) was suitable for Diversisporales fungi. Therefore, we anticipate that the refining the P concentration will contribute to future culture collections of a wide range of AM fungi.

show abstract

Secretion of acid phosphatase from extraradical hyphae of the arbuscular mycorrhizal fungus Rhizophagus clarus is regulated in response to phosphate availability

Cited by 61 publications

References 35 publications

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Effect of arbuscular mycorrhizal fungi on the physiological functioning of maize under zinc-deficient soils

Alleviation of cadmium and lead stress by A‐mycorrhizal fungi and rock phosphate on maize (Zea mays L.) growth under calcareous soil conditions

Phosphorus is a critical factor of the in vitro monoxenic culture method for a wide range of arbuscular mycorrhizal fungi culture collections

Contact Info

Product

Resources

About