Secretion of fucosylated oligosaccharides related to the H antigen by human gastric cells

Madrid, Juan Francisco; Leis, Olga; Díaz‐Flores, Lucio; Hernández, Francisco Velasco

doi:10.1007/s004180050291

Cited by 11 publications

(9 citation statements)

References 31 publications

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“…The use of lectins as markers of blood groups can be controversial. The lectin binding patterns of UEA-I, LTA, and AAA are not identical to that previously described for the anti-H antibody in the human fundic glands (Madrid et al 1998b). All the structures labeled by the antibody were also positive for the lectins, but some additional structures were labeled only by the lectins.…”

Section: Localization Of Fucosylated Oligosaccharidescontrasting

confidence: 51%

“…Thus, the transitional cells can contain secretory granules with a different electron-lucent/electron-dense core ratio. The last step would be the absence of electronlucent regions, originating the chief cells (Suzuki et al 1983;Karam and Leblond 1993c;Madrid et al 1990Madrid et al ,1998b. Our study demonstrates that the composition of the two different compartments (electrondense and electron-lucent) of the granules in the transitional and mucous neck cells is different but that, at the same time, they are similar compared with the corresponding one of the other cell type.…”

Section: Implications Of the Biphasic Nature Of The Granulesmentioning

confidence: 61%

“…Because this is not the subject of this work, the method will not be described. The sections were stained according to Madrid et al (1994Madrid et al ( ,1998b.…”

Section: Immunocytochemical Labelingmentioning

confidence: 99%

“…The transitional cells have been considered as a stage in the maturation process from the mucous neck cells to the chief cells (Suzuki et al 1983;Karam and Leblond 1993c;Madrid et al 1990Madrid et al ,1998b. During this maturation process, the electron-dense core of the secretory granules or mucous neck cells progressively increases in size as the cells descend through the gland.…”

Section: Implications Of the Biphasic Nature Of The Granulesmentioning

confidence: 99%

“…In the human fundic region of the stomach, the glands are composed of mucous neck cells, chief cells, parietal cells, and endocrine cells. More recently, the existence of transitional or prezymogen cells has been described (Rubin et al 1968;Cornaggia et al 1986;Karam and Leblond 1993a,b;Ogata 1997;Yang et al 1997;Madrid et al 1990Madrid et al ,1998b. These transitional cells can be considered as an intermediate stage in the hypothetical differentiation process from the mucous neck cells to the chief cells.…”

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confidence: 99%

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Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Madrid

Leis

Díaz‐Flores

et al. 1998

J Histochem Cytochem.

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SUMMARYThe oligosaccharides of the mucous gastric glycoproteins are involved in the protection of the gastric mucosa and are altered in different diseases. Therefore, it is important to know their composition in health, to better determine the alterations induced by the disease. Moreover, analysis of the molecular composition of the fundic gland cells has been previously used to obtain new insights into the origin of the different cell types. The aim of the present study was the localization in the subcellular structures of the fucose residues of the oligosaccharides in human fundic glands. For this, lectin cytochemical methods were used at the light and electron microscopic levels. They were combined with enzymatic and chemical treatments to characterize the nature of the oligosaccharide chains containing the fucose residues. The presence of this carbohydrate belonging to N-or O-linked oligosaccharides has been demonstrated in the secretory granules of the surface, gastric pit, mucous neck, and transitional cells of the fundic mucosa, and in the intracellular canaliculi and tubulovesicular system of the parietal cells. These fucose residues were added in the trans-Golgi regions to the elongating chains. The term "mucosal defense" refers to the factors that permit the gastric mucosa to withstand frequent exposure to substances with a wide range of properties including, e.g., pH, osmolarity, and detergent properties. The first level of defense consists of the factors secreted into the lumen, including acid, bicarbonate, mucus, immunoglobulins, and other antibacterial substances (Wallace and Granger 1996). Mucous secretions form a barrier that traps bicarbonate secreted by the epithelium and stops acid retrodiffusion from the gastric lumen. Moreover, bacteria become trapped in the mucus and are eventually excreted in the feces (Wallace and Granger 1996).

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Section: Localization Of Fucosylated Oligosaccharidescontrasting

confidence: 51%

Section: Implications Of the Biphasic Nature Of The Granulesmentioning

confidence: 61%

“…Because this is not the subject of this work, the method will not be described. The sections were stained according to Madrid et al (1994Madrid et al ( ,1998b.…”

Section: Immunocytochemical Labelingmentioning

confidence: 99%

Section: Implications Of the Biphasic Nature Of The Granulesmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Madrid

Leis

Díaz‐Flores

et al. 1998

J Histochem Cytochem.

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Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands

Gomez-Santos

Alonso

Díaz‐Flores

et al. 2018

The Anatomical Record

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The fundic glands of the stomach contain two types of mucous cells: surface mucous cells (SMCs) located at the surface of the stomach and the pits, and mucous neck cells (MNCs) situated in the neck of the glands. They produce mucins, highly glycosylated proteins. Very little is known about the glycan composition of these mucins and of gastric secretion in general. We used several lectins combined with deglycosylation pretreatments to analyze the glycan composition of SMCs and MNCs. The results showed the presence of terminal sialic acid and subterminal Gal and GalNAc, which is consistent with previous knowledge about glycosylation in mucins. Our results also support previous reports that showed a different expression of mucins in the SMCs, depending on their superficial or deep location in the pit. Some lectins labeled only the perinuclear region of the SMCs, but not the apical region, where the secretory granules are stored. This suggests that the lectins are labeling sugar residues that are accessible to lectins during the first steps of glycan synthesis, which occurs in the endoplasmic reticulum and Golgi apparatus. Our results indicate that SMCs and MNCs produce a mucus secretion with a different glycoconjugate composition. The secretion is more varied in SMCs. As our results coincide with what we know about glycosylation of mucins, we can conclude that most of the glycans detected belong to mucins, and the differences in glycosylation observed in each cell type may be due, mainly, to the different secreted mucins. Anat Rec, 301:2128–2144, 2018. © 2018 Wiley Periodicals, Inc.

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Glycosylation patterns are sexually dimorphic throughout development of the olfactory system in Manduca sexta

Gibson

Hildebrand

Tolbert

2004

J of Comparative Neurology

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In the moth Manduca sexta, development of the adult olfactory system depends on complex interactions between olfactory receptor neurons in the antenna, antennal-lobe neurons in the brain, and several classes of glial cells. As one approach to characterizing molecules that may play roles in these interactions, we used lectins to screen antennae and antennal lobes at different stages of adult development. We find that each of the major neural cell types has a distinct pattern of labeling by lectins. Effects of enzymatic and other treatments on lectin labeling lead us to conclude that the predominant lectin ligands are: glycosphingolipids and an O-linked, fucose-containing glycoprotein on axons of olfactory receptor neurons, O-linked glycoproteins on antennal-lobe neurons, and N-linked glycoproteins on all classes of glial cells in the primary olfactory pathway. Wheat germ agglutinin labels all olfactory axons uniformly during much of development, but labeling becomes restricted to the pheromone-responsive olfactory receptor neurons in the adult male. Succinylated WGA reveals differences in these axon classes earlier, as glomerului develop from protoglomeruli. The adult female displays a less pronounced difference in labeling of axons targeting ordinary and sexually dimorphic glomeruli. Differences in labeling of receptor axons targeted to ordinary and sexually dimorphic glomeruli may be correlated with differences in function or connectivity in different regions of the antennal lobe.

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Secretion of fucosylated oligosaccharides related to the H antigen by human gastric cells

Cited by 11 publications

References 31 publications

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Lectin-Gold Localization of Fucose Residues in Human Gastric Mucosa

Different Glycoconjugate Content in Mucus Secreting Cells of the Rat Fundic Gastric Glands

Glycosylation patterns are sexually dimorphic throughout development of the olfactory system in Manduca sexta

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