Secretion of granule proteins from eosinophils and neutrophils is increased in asthma

Carlson, Marie; Håkansson, Lena; Peterson, Christer; Stålenheim, Gunnemar; Venge, Per

doi:10.1016/0091-6749(91)90209-7

Cited by 132 publications

(89 citation statements)

References 22 publications

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“…Other investigators have reported on the number of eosinophils and the serum concentration of eosinophilderived proteins in asthmatic adults [12][13][14][15][16][17][18], and in asthmatic children [4,5,18,19]. A comparison of the number of eosinophils and the concentration of eosinophil-derived proteins between asthmatic children and healthy controls was described previously [4,5].…”

Section: Discussionmentioning

confidence: 94%

Eosinophils and eosinophil-derived proteins in children with moderate asthma

Hovenga²,

Gerritsen³

et al. 1996

Eur Respir J

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E Eo os si in no op ph hi il ls s a an nd d e eo os si in no op ph hi il l--d de er ri iv ve ed d p pr ro ot te ei in ns s i in n cThe number of eosinophils, serum ECP and EDN, and urinary EDN were determined in 22 children with stable, allergic asthma, aged 4-14 yrs, and in 17 agematched healthy controls. Symptoms were monitored, the peak expiratory flow rate (PEFR) was recorded in the younger children, and lung function tests (forced expiratory volume in one second (FEV1) and the provocative concentration of histamine causing a 20% fall in FEV1 (PC20)) were performed in the older children. None of the asthmatic children had respiratory symptoms. PEFR was not significantly different in asthmatic children compared to controls. The FEV1 % predicted was significantly lower compared to controls.The number of eosinophils, serum ECP and EDN, and urinary EDN were significantly higher in asthmatic children compared with controls. After correction of serum ECP and EDN, and urinary EDN for the number of eosinophils, the differences between patients and controls disappeared. The nocturnal PEFR and the FEV1 were significantly related to urinary EDN.The results suggest that serum and urinary concentration of eosinophil-derived proteins can be determined instead of the number of eosinophils to support the diagnosis of asthma in childhood. The urinary concentration of eosinophil-derived neurotoxin can be especially valuable in young children, because in this age group quantification of lung function cannot be performed and blood sampling can be difficult.

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Section: Discussionmentioning

confidence: 94%

Eosinophils and eosinophil-derived proteins in children with moderate asthma

Hovenga²,

Gerritsen³

et al. 1996

Eur Respir J

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“…There is increasing evidence of neutrophil participation in asthma and the allergic process (25). For instance, peripheral blood neutrophils are activated during active asthma (26), after exercise-induced bronchospasm (27), and during both early and late allergen-induced asthmatic reactions (28). Several studies have reported evidence linking the presence of neutrophils with airway damage and dysfunction (29 -31).…”

Section: E Osinophil Cationic Protein (Ecp)mentioning

confidence: 99%

Neutrophils as a Novel Source of Eosinophil Cationic Protein in IgE-Mediated Processes

Monteseirı́n

Vega

Chacón

et al. 2007

The Journal of Immunology

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The production of eosinophil cationic protein (ECP) in IgE-mediated diseases has been associated mainly with eosinophils, although no IgE-dependent ECP release has been observed in these cells. Because there is increasing evidence of neutrophil participation in allergic processes, we have examined whether human neutrophils from allergic patients were able to produce ECP by an IgE-dependent mechanism. After challenge with specific Ags to which the patients were sensitized, ECP release was detected in the culture medium. Furthermore, intracellular protein was detected by flow cytometry, immunofluorescence staining, and Western blotting. Expression at both mRNA and de novo protein synthesis were detected, respectively, by RT-PCR and radiolabeling with 35S. Ag effect was mimicked by cell treatment with anti-IgE Abs or Abs against FcεRI and galectin-3 (FcεRI>galectin-3), but not against FcεRII. These observations represent a novel view of neutrophils as possible source of ECP in IgE-dependent diseases.

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“…Eosinophil protein X (EPX) is released from eosinophil granulocytes and has been used to assess eosinophil activity and monitor inflammation in bronchial asthma [13,14]. It is a chemically stable protein that can be detected in the urine of healthy subjects and has been found to be significantly increased in children with acute asthma [15].…”

mentioning

confidence: 99%

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

Severien¹,

Artlich²,

Jonas³

et al. 2000

Eur Respir J

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Measurement of leukotriene E 4 (LTE 4 ) in urine is a noninvasive method for assessing changes in the rate of total body cysteinyl leukotriene production. Eosinophil protein X (EPX) has been used to assess eosinophil activity and monitor inflammation in bronchial asthma. The aim of the study was to look for differences in urinary LTE 4 and EPX concentrations between children with stable atopic asthma and healthy controls and to compare asthmatic children with different disease severity. In addition the relationship was evaluated between urinary LTE 4 amd EPX levels and lung function.LTE 4 was also measured (enzyme immunoassay) together with EPX (radioimmunoassay) in urine and lung function tests were carried out in children with mild asthma (steroid-naive) (n=49), moderate to severe asthma (using inhaled steroids) (n=31) and healthy control subjects (n=28).Urinary leukotriene E 4 (LTE 4 ) was significantly higher in children with asthma than in controls (median [25±75 percentile] 238.5 (126.5±375.7) SD 191.8 versus 189 (51±253.2) SD 131.7 pg . mg -1 creatinine; p=0.021). Urinary EPX was also significantly increased in asthmatic children compared with controls (85.5 [64±131.5] SD 76.2 versus 48.5 [43.2±90] 112.1 mg . mmol -1 creatinine; p=0.006). There were no differences in urinary LTE 4 and EPX between the group of mild and the group of moderate to severe asthmatic children. There were significant associations between the urinary LTE 4 and intrathoracic gas volume (ITGV), residual volume (RV), forced expiratory volume in one second (FEV1), forced expiratory capacity (FVC) and maximum expiratory flow rate at 25% of vital capacity (MEF25).Urinary EPX was only correlated with maximum expiratory flow rate at 75% of vital capacity (MEF75). Thus measurement of urinary LTE 4 may predict the degree of airflow obstruction in asthmatic children. Urinary LTE 4 and EPX are useful markers of airway inflammation and can be helpful in guiding asthma management. There was no correlation between LTE 4 and EPX levels. Eur Respir J 2000; 16: 588±592.

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Secretion of granule proteins from eosinophils and neutrophils is increased in asthma

Cited by 132 publications

References 22 publications

Eosinophils and eosinophil-derived proteins in children with moderate asthma

Eosinophils and eosinophil-derived proteins in children with moderate asthma

Neutrophils as a Novel Source of Eosinophil Cationic Protein in IgE-Mediated Processes

Urinary excretion of leukotriene E4 and eosinophil protein X in children with atopic asthma

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