Secretion of proteolytic enzymes by three phytopathogenic microorganisms

Kudryavtseva, N. N.; Sofyin, A. V.; Revina, T. A.; Gvozdeva, E. L.; Ievleva, E. V.; Valueva, T. A.

doi:10.1134/s0003683813050074

Cited by 18 publications

(6 citation statements)

References 23 publications

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“…Plants are attacked during their lifetime by microorganisms that negatively impact yield and quality of agricultural crops. There is a clear relation between the ability of pathogens to invade and spread in plant tissues and their spectrum of hydrolytic enzyme activities (Table , Kudryavtseva et al, ; Protsenko et al, ; Yarullina et al, ). While sporadic cases or outbreaks can arise from bacterial, viral, or parasitic contamination, the latter two require animal hosts for proliferation and cannot grow on or within plants.…”

Section: Introductionmentioning

confidence: 99%

“…Some highly virulent plant pathogens have evolved to produce effector molecules that interfere with signaling pathways controlling MAMP‐triggered immunity (MTI). Many plant pathogens are also able to produce a range of hydrolytic enzymes (Table ) capable of degrading plant cell wall components, which allow them to invade and spread in plant tissues (Kudryavtseva et al, ; Protsenko et al, ; Yarullina et al, ). However, biofilm production (described below) is a key component in pathogenesis by plant pathogens, promoting virulence, and protection against plant defense responses.…”

Section: Introductionmentioning

confidence: 99%

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Human pathogens in plant biofilms: Formation, physiology, and detection

Ximenes

Hoagland

et al. 2017

Biotech & Bioengineering

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Fresh produce, viewed as an essential part of a healthy life style is usually consumed in the form of raw or minimally processed fruits and vegetables, and is a potentially important source of food-borne human pathogenic bacteria and viruses. These are passed on to the consumer since the bacteria can form biofilms or otherwise populate plant tissues, thereby using plants as vectors to infect animal hosts. The life cycle of the bacteria in plants differs from those in animals or humans and results in altered physiochemical and biological properties (e.g., physiology, immunity, native microflora, physical barriers, mobility, and temperature). Mechanisms by which healthy plants may become contaminated by microorganisms, develop biofilms, and then pass on their pathogenic burden to people are explored in the context of hollow fiber microfiltration by which plant-derived microorganisms may be recovered and rapidly concentrated to facilitate study of their properties. Enzymes, when added to macerated plant tissues, hydrolyze or alter macromolecules that would otherwise foul hollow-fiber microfiltration membranes. Hence, microfiltration may be used to quickly increase the concentration of microorganisms to detectable levels. This review discusses microbial colonization of vegetables, formation and properties of biofilms, and how hollow fiber microfiltration may be used to concentrate microbial targets to detectable levels. The use of added enzymes helps to disintegrate biofilms and minimize hollow fiber membrane fouling, thereby providing a new tool for more time effectively elucidating mechanisms by which biofilms develop and plant tissue becomes contaminated with human pathogens. Biotechnol. Bioeng. 2017;114: 1403-1418. © 2017 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Human pathogens in plant biofilms: Formation, physiology, and detection

Ximenes

Hoagland

et al. 2017

Biotech & Bioengineering

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“…The activity of pathogen enzymes, proteases, has been directly correlated to the severity of plant disease, which indicates their important role in pathogen colonization. The proteases destroy the plant cell wall structural proteins, allowing the pathogen to obtain nutrients for its growth, as well as to evade plant defenses [22,23] (Figure 2a). The inhibition of pathogenic enzymes by the plant PIs constitutes a plant defense mechanism.…”

Section: Legume Responses To Pathogen Attackmentioning

confidence: 99%

Legumes Protease Inhibitors as Biopesticides and Their Defense Mechanisms against Biotic Factors

Rodríguez‐Sifuentes

Marszalek

Chuck‐Hernández

et al. 2020

IJMS

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Legumes are affected by biotic factors such as insects, molds, bacteria, and viruses. These plants can produce many different molecules in response to the attack of phytopathogens. Protease inhibitors (PIs) are proteins produced by legumes that inhibit the protease activity of phytopathogens. PIs are known to reduce nutrient availability, which diminishes pathogen growth and can lead to the death of the pathogen. PIs are classified according to the specificity of the mechanistic activity of the proteolytic enzymes, with serine and cysteine protease inhibitors being studied the most. Previous investigations have reported the efficacy of these highly stable proteins against diverse biotic factors and the concomitant protective effects in crops, representing a possible replacement of toxic agrochemicals that harm the environment.

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“…Serine proteases constitute the most well-studied protease family, comprising over 33% of all identified proteases (Yike, 2011). Extracellular fungal proteases concentrate in the serine family (Chandrasekaran et al ., 2016), which includes the common subtilisin and trypsin types (Kudryavtseva et al ., 2013). Alkaline proteases contain either a serine or metallo- centre (Sharma et al ., 2017).…”

Section: Fungal Pathogenic Enzymesmentioning

confidence: 99%

Seedsvsfungi: an enzymatic battle in the soil seedbank

Pollard

2018

Seed Sci. Res.

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Depleting the soil weed seedbank is an important integrated weed management strategy that has the potential to foster lasting weed control. Long-term dormancy and decay resistance of weed seeds pose a challenge to weed eradication efforts. Select soil fungi have been shown to cause significant decay of weed seeds. The physical and chemical mechanisms by which seeds in the seedbank defend themselves against pathogens have been well researched. However, very few studies have purposefully investigated the biochemical defence response of seeds. Enzyme-based biochemical seed defences have been detected in dormant and non-dormant seeds, and research supports their function in pathogen defence. This review summarizes current knowledge of the seed defence enzymes polyphenol oxidase, peroxidase, chitinase and oxalate oxidase. The fungal enzymes chitinase, protease and xylanase that function in pathogenesis of seeds in the soil seedbank are also reviewed. Progress in the development and standardization ofin situenzyme analyses fosters our understanding of actual enzyme activity present in soils, while high-throughput microplate techniques promote efficiency and enable greater scope. Application of genomic, proteomic and transcriptomic techniques to glean a deeper and more holistic understanding of the enzymatic interactions of weed seeds and soil fungi in the soil seedbank will support the development of improved integrated weed management strategies.

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Secretion of proteolytic enzymes by three phytopathogenic microorganisms

Cited by 18 publications

References 23 publications

Human pathogens in plant biofilms: Formation, physiology, and detection

Human pathogens in plant biofilms: Formation, physiology, and detection

Legumes Protease Inhibitors as Biopesticides and Their Defense Mechanisms against Biotic Factors

Seedsvsfungi: an enzymatic battle in the soil seedbank

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