Secretion of tartrate‐resistant acid phosphatase by osteoclasts correlates with resorptive behavior

Abstract

There have been dramatic advances recently in our understanding of the regulation of osteoclastic differentiation. However, much less is known of the mechanisms responsible for the induction and modulation of resorptive behavior. We have developed a strategy whereby osteoclasts can be generated in vitro and released into suspension in a fully-functional state. We now exploit this approach to show that tartrate-resistant acid phosphatase (TRAP) is released by osteoclasts during bone resorption. TRAP release was inhibited by the secretion-inhibitor Brefeldin A, and was not accompanied by LDH release. This suggests that TRAP release is due to secretion, rather than cell death. Consistent with this, TRAP secretion was stimulated by resorbogenic cytokines, was inhibited by the resorption-inhibitor calcitonin, and correlated with excavation of the bone surface. We found that, in contrast to incubation on bone, incubation on plastic, glass, or vitronectin-coated plastic substrates did not induce secretion of TRAP. This suggests that the induction of resorptive behavior in osteoclasts depends upon stimulation by bone matrix of a putative osteoclastic "mineral receptor." Release of TRAP by osteoclasts thus represents not only a productive approach to the analysis of the mechanisms that modulate the rate of resorptive activity, but also a system whereby the mechanism through which bone substrates induce resorptive behavior can be identified.

In vitro effects of particulate bone substitute materials on the resorption activity of human osteoclasts

“…TRAP-staining provides standard cytochemical detection and quantification of osteoclasts (Kirstein et al, 2006).…”

“…In order to quantify osteoclast-like cells, TRAP-positive cells were counted by light microscopy (BX60 microscope, Olympus, Tokyo, Japan). Reddish to brownish staining, featuring at least 3 nuclei, were classified as TRAP-positive (Kirstein et al, 2006). For evaluation, TRAP-positive cells were summarized per group and donor.…”

Bone regenerative effect of aqueous Cynanchum wilfordii extract in receptor activator of nuclear factor-κB ligand-induced osteoclast differentiation and estrogen deficiency-induced osteoporosis

“…M-CSF plays a crucial role in the proliferation and survival of osteoclast precursors and the constitutive expression of RANK [4]. resistant acid phosphatase(TRAP), a osteoclast differentiation, during bone resorption and the secretion is found to correlate positively with resorptive behavior [5]. Excessive osteoclastogenesis is associated with the In preclinical and clinical research, bone turnover biomarkers are into bone formation and resorption markers [7].…”

“…Consequently, bone homeostasis maintains continuous remodeling of osteoblasts and osteoclasts, which is ultimately by the proliferation and differentiation of precursors of associated cells. Especially, osteoclasts, which are resorbing multinucleated giant cells, are differentiated from macrophage lineage crucial cytokines: macrophage colony-stimulating factor (M activator of nuclear factor-κ B (NFplays a crucial role in the proliferation and survival of osteoclast precursors and the constitutive expression Osteoclasts secrete tartrate-resistant acid phosphatase(TRAP), a biomarker of osteoclast differentiation, during bone resorption and the secretion is found to correlate positively with resorptive behavior [5]. Excessive osteoclastogenesis pathogenesis of osteoporosis [6].…”

Effect of titanium particles on osteoclast activity in vitro

“…TraP has been used as a marker of osteoclast function for more than 20 years, and has been shown to be a specific and sensitive indicator of bone resorption (24,25). in the present study, titanium particles dose-dependently decreased TraP expression, indicating the inhibition of osteoclast activity.…”