Secretogranin II is overexpressed in advanced prostate cancer and promotes the neuroendocrine differentiation of prostate cancer cells

Courel, Maïté; Yamani, Fatima-Zohra El; Dionne‐Laporte, Alexandre; Fatemi, Hinde El; Delestre, Charlène; Montero-Hadjadje, Maïté; Tazi, Fadl; Amarti, Afaf; Magoul, Rabia; Chartrel, Nicolas; Anouar, Youssef

doi:10.1016/j.ejca.2014.09.009

Cited by 16 publications

(14 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CAV2 was a major component of the inner surface of caveolae, and the expression of CAV2 was necessary for the control of E2-dependent cellular proliferation in breast cancer (Totta et al, 2016). SCG2 was a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins, and it might contribute to the neuroendocrine differentiation by promoting the formation of secretory granules and the proliferation of prostate cancer cells (Courel et al, 2014). The SLC6A1 gene encoded a gamma-aminobutyric acid (GABA) transporter, which removes GABA from the synaptic cleft (Hirunsatit et al, 2009), but there have been no studies in relation to cancer as of now.…”

Section: Discussionmentioning

confidence: 99%

Recurrence‐associated gene signature optimizes recurrence‐free survival prediction of colorectal cancer

et al. 2017

View full text Add to dashboard Cite

High throughput gene expression profiling has showed great promise in providing insight into molecular mechanisms. Metastasis‐related mRNAs may potentially enrich genes with the ability to predict cancer recurrence, therefore we attempted to build a recurrence‐associated gene signature to improve prognostic prediction of colorectal cancer (CRC). We identified 2848 differentially expressed mRNAs by analyzing CRC tissues with or without metastasis. For the selection of prognostic genes, a LASSO Cox regression model (least absolute shrinkage and selection operator method) was employed. Using this method, a 13‐mRNA signature was identified and then validated in two independent Gene Expression Omnibus cohorts. This classifier could successfully discriminate the high‐risk patients in discovery cohort [hazard ratio (HR) = 5.27, 95% confidence interval (CI) 2.30–12.08, P < 0.0001). Analysis in two independent cohorts yielded consistent results (GSE14333: HR = 4.55, 95% CI 2.18–9.508, P < 0.0001; GSE33113: HR = 3.26, 95% CI 2.16–9.16, P = 0.0176). Further analysis revealed that the prognostic value of this signature was independent of tumor stage, postoperative chemotherapy and somatic mutation. Receiver operating characteristic (ROC) analysis showed that the area under ROC curve of this signature was 0.8861 and 0.8157 in the discovery and validation cohort, respectively. A nomogram was constructed for clinicians, and did well in the calibration plots. Furthermore, this 13‐mRNA signature outperformed other known gene signatures, including oncotypeDX colon cancer assay. Single‐sample gene‐set enrichment analysis revealed that a group of pathways related to drug resistance, cancer metastasis and stemness were significantly enriched in the high‐risk patients. In conclusion, this 13‐mRNA signature may be a useful tool for prognostic evaluation and will facilitate personalized management of CRC patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Recurrence‐associated gene signature optimizes recurrence‐free survival prediction of colorectal cancer

et al. 2017

View full text Add to dashboard Cite

show abstract

“…3.5 | Immunohistochemistry and effects of IL-6 on the expression of SCG2 protein and mRNA (Berard, Severini, & Coombs, 2015;Courel et al, 2014). To verify the results of screening DEGs in transcriptome sequencing, we first assessed SCG2 immunoreactivity in cancer and paracancerous tissues.…”

Section: Il-6 Expression Was Upregulated In Facamentioning

confidence: 99%

Interleukin‐6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI‐H295R cells

Xian

Zhang

Peng

et al. 2020

Journal Cellular Physiology

View full text Add to dashboard Cite

The purpose of this study was to investigate the potential mechanism of interleukin‐6 (IL‐6) on the stimulation of excessive androgen secretion in human NCI‐H295R adrenocortical cells. We performed transcriptome sequencing of cancer and paracancerous tissues obtained from functional adrenal cortical adenomas. The secretion of dehydroepiandrosterone sulfate (DHEAS) in NCI‐H295R cells was detected by a chemiluminescence assay. The expression of messenger RNA (mRNA) was detected by real‐time polymerase chain reaction and that of protein was detected by western blotting. The expression of secretogranin II (SCG2) and IL‐6 were significantly increased in cancer tissues. Upregulation of mRNA and protein levels of AKR1C3, CYP11A, CYP17A1, 3βHSD, and SULT2A1 was observed after stimulation with IL‐6. IL‐6 could also increase the expression of StAR mRNA and proteins. Our results suggest that IL‐6 can promote androgen secretion by regulating the expression of genes related to androgen pathways.

show abstract

“…Clinically, it has been shown that EM66 is present in tumoural chromaffin cells and represents a sensitive diagnostic and pronostic marker of pheochromocytoma . Furthermore, EM66 and its precursor SgII are highly expressed in advanced prostate adenocarcinoma and may contribute to the neuroendocrine differentiation of prostate cancer cells by promoting the formation of secretory granules and the proliferation of tumoural cells …”

Section: Introductionmentioning

confidence: 99%

“…19 Furthermore, EM66 and its precursor SgII are highly expressed in advanced prostate adenocarcinoma and may contribute to the neuroendocrine differentiation of prostate cancer cells by promoting the formation of secretory granules and the proliferation of tumoural cells. 20 Neuroanatomical studies have revealed that, in the jerboa, EM66producing neurones are present in various hypothalamic structures, including the suprachiasmatic, supraoptic, parvocellular paraventricular (pPVN) and arcuate (ARC) nuclei, and the lateral hypothalamic area (LHA). 18 Similarly, in the rat hypothalamus, EM66-containing neurones are found in the pPVN, preoptic area, ARC and in the LHA.…”

Section: Introductionmentioning

confidence: 99%

A potential role for the secretogranin II‐derived peptide EM66 in the hypothalamic regulation of feeding behaviour

Trebak

Dubuc

Arabo

et al. 2017

J Neuroendocrinology

View full text Add to dashboard Cite

EM66 is a conserved 66-amino acid peptide derived from secretogranin II (SgII), a member of the granin protein family. EM66 is widely distributed in secretory granules of endocrine and neuroendocrine cells, as well as in hypothalamic neurones. Although EM66 is abundant in the hypothalamus, its physiological function remains to be determined. The present study aimed to investigate a possible involvement of EM66 in the hypothalamic regulation of feeding behaviour. We show that i.c.v. administration of EM66 induces a drastic dose-dependent inhibition of food intake in mice deprived of food for 18 hours, which is associated with an increase of hypothalamic pro-opiomelanocortin (POMC) and melanocortin-3 receptor mRNA levels and c-Fos immunoreactivity in the POMC neurones of the arcuate nucleus. By contrast, i.c.v. injection of EM66 does not alter the hypothalamic expression of neuropeptide Y (NPY), or that of its Y1 and Y5 receptors. A 3-month high-fat diet (HFD) leads to an important decrease of POMC and SgII mRNA levels in the hypothalamus, whereas NPY gene expression is not affected. Finally, we show that a 48 hours of fasting in HFD mice decreases the expression of POMC and SgII mRNA, which is not observed in mice fed a standard chow. Taken together, the present findings support the view that EM66 is a novel anorexigenic neuropeptide regulating hypothalamic feeding behaviour, at least in part, by activating the POMC neurones of the arcuate nucleus.

show abstract

Secretogranin II is overexpressed in advanced prostate cancer and promotes the neuroendocrine differentiation of prostate cancer cells

Abstract: The present data show that SgII is highly expressed in advanced PCa and may contribute to the neuroendocrine differentiation by promoting the formation of secretory granules and the proliferation of PCa cells.

Cited by 16 publications

References 40 publications

Recurrence‐associated gene signature optimizes recurrence‐free survival prediction of colorectal cancer

Recurrence‐associated gene signature optimizes recurrence‐free survival prediction of colorectal cancer

Interleukin‐6 increases adrenal androgen release by regulating the expression of steroidogenic proteins in NCI‐H295R cells

A potential role for the secretogranin II‐derived peptide EM66 in the hypothalamic regulation of feeding behaviour

Contact Info

Product

Resources

About