Secretomes as an emerging class of bioactive ingredients for enhanced cosmeceutical applications

Tan, Kei‐Xian; Chang, Trixie; Lin, Xiangliang

doi:10.1111/exd.14570

Cited by 14 publications

(11 citation statements)

References 95 publications

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“…In particular, the results of previous in vitro studies and preclinical or clinical applications have suggested that the retention of cellular viability or the presence of the original cellular structures was not required to conserve specific therapeutic functions [ 31 , 32 , 38 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 ]. Therefore and as mentioned above, further potential applications inspired by dermal progenitor cytotherapies comprise the topical use of cell-derived cell-free biological complexes (e.g., cell secretomes, exosome fractions) in cutaneous regenerative medicine [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Retrospectives on Three Decades of Safe Clinical Experience with Allogeneic Dermal Progenitor Fibroblasts: High Versatility in Topical Cytotherapeutic Care

et al. 2023

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Allogeneic dermal progenitor fibroblasts constitute cytotherapeutic contenders for modern cutaneous regenerative medicine. Based on advancements in the relevant scientific, technical, and regulatory fields, translational developments have slowly yet steadily led to the clinical application of such biologicals and derivatives. To set the appropriate general context, the first aim of this study was to provide a current global overview of approved cell and gene therapy products, with an emphasis on cytotherapies for cutaneous application. Notable advances were shown for North America, Europe, Iran, Japan, and Korea. Then, the second and main aim of this study was to perform a retrospective analysis on the various applications of dermal progenitor fibroblasts and derivatives, as clinically used under the Swiss progenitor cell transplantation program for the past three decades. Therein, the focus was set on the extent and versatility of use of the therapies under consideration, their safety parameters, as well as formulation options for topical application. Quantitative and illustrative data were summarized and reported for over 300 patients treated with various cell-based or cell-derived preparations (e.g., progenitor biological bandages or semi-solid emulsions) in Lausanne since 1992. Overall, this study shows the strong current interest in biological-based approaches to cutaneous regenerative medicine from a global developmental perspective, as well as the consolidated local clinical experience gathered with a specific and safe allogeneic cytotherapeutic approach. Taken together, these current and historical elements may serve as tangible working bases for the further optimization of local and modern translational pathways for the provision of topical cytotherapeutic care.

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Section: Discussionmentioning

confidence: 99%

Retrospectives on Three Decades of Safe Clinical Experience with Allogeneic Dermal Progenitor Fibroblasts: High Versatility in Topical Cytotherapeutic Care

et al. 2023

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“…In this regard, SCM has been shown to contain a number of growth factors and cytokines that may contribute to the beneficial effects of SCM (54,55). Recent studies have also shown that SCM contains microvesicles or extracellular vesicles (EVs) and that the EVs obtained from MSCs exert beneficial effects by transferring biological cargo, including cytoskeleton proteins, signaling proteins, lipids, enzymes, transcription factors, mRNA, microRNAs, long non-coding RNAs, DNA and metabolites, to host cells (56,57). However, the exact factors responsible for the effects observed in the present study remain unknown and require further investigation (54).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of LPS‑induced NLRP3 inflammasome activation by stem cell‑conditioned culture media in human gingival epithelial cells

Sun

Wang

2023

Mol Med Rep

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Interleukin (IL)-1β is a pathogenic factor associated with the destruction of periodontal tissue in periodontitis. IL-1β processing is regulated by cytosolic machinery known as the inflammasome. Porphyromonas gingivalis infection and lipopolysaccharide (LPS) have an important role in the destruction of periodontal tissue in periodontitis. P. gingivalis infection and LPS have been reported to activate the NOD-like receptor family pyrin domain-containing protein 3 (NLRP3) inflammasome in human oral cells. Stem cell therapy exhibits anti-inflammatory effects and stem cell-conditioned culture media (SCM) shows similar beneficial effects. The present study tested the hypothesis that SCM inhibits activation of the inflammasome and protects human gingival epithelial cells (GECs) against LPS-induced inflammatory damage. Human GECs were treated with or without LPS plus SCM or control cell media. NLPR3 inflammasome components and inflammatory factors were measured by western blotting and immunofluorescence. The present study revealed that LPS induced an increase in the expression of inflammasome components, NLRP3, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) and caspase-1. Co-immunoprecipitation revealed increased binding of NLRP3 and ASC, and immunofluorescence showed an increased co-localization of ASC and caspase-1, suggesting that LPS stimulated assembly of the NLRP3 inflammasome. SCM inhibited the overexpression and assembly of NLRP3 inflammasome components induced by LPS. Furthermore, SCM blocked the increase in IL-1β production induced by LPS and inhibited the translocation of the inflammatory factor, NF-κB, into the nuclei. Consequently, SCM protected cells against LPS-induced damage, as suggested by the recovery of disturbed E-cadherin staining pattern, which indicates a disruption in epithelial integrity. In conclusion, treatment with SCM may attenuate LPS-induced inflammatory damage in human GECs via inhibition of NLRP3 inflammasome activation, suggesting a potential therapeutic use for SCM.

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“…Further, it is reported that in a mouse model, injected ADSCs survived for 28 days, suggesting the possible long-term active life of ADSCs in injected skin. In addition, a conditioned medium known as secretome, which contains extracellular vesicles, such as exosome, is reported to have a variety of biological activities for skin repair and rejuvenation [12]. Secretome isolated from ADSCs contains a wide range of cytokines and growth factors that are beneficial in reversing aged skin symptoms by inducing epidermal keratinocyte, dermal fibroblast activation, and proliferation [13].…”

Section: Discussionmentioning

confidence: 99%

A Single Intradermal Injection of Autologous Adipose-Tissue-Derived Stem Cells Rejuvenates Aged Skin and Sharpens Double Eyelids

Ichihashi,

Tanaka,

Iizuka

et al. 2023

JPM

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Facial skin aging is the most visible manifestation of aging in the body. In this study, we aimed to rejuvenate aging skin via a one-time intradermal injection of autologous adipose-derived stem cells (ADSCs). Eight patients were enrolled for study. Photographs of patients taken immediately before and 1, 3, 6, and 12 months after ADSC injections were comparatively evaluated for visible skin manifestations. ADSCs were cultured from the abdominal-skin-derived subcutaneous fat tissue, and 1 × 108 cultured ADSCs were injected intradermally into the facial skin. Cultured myoblasts were incubated with the supernatant derived from ADSCs, and the effect was evaluated via glucose consumption and lactic acid production in the medium. Eight cases showed the shallowing and disappearance of wrinkles, including those of the glabella, lower eyelids, crow`s feet, and forehead and nasolabial grooves, a month to several months after treatment. Double eyelids became prominent, and facial pores significantly reduced in size. These effects lasted for over one year. Myoblasts cultured in the presence of an ADSC-derived exosome were activated compared to that of ADSCs cultured without supernatant. The result supports the role of muscle in ADSC skin rejuvenation. The present study first reports that a single intradermal administration of cultured ADSCs rejuvenates aged facial skin over the course of one year. Further, patients exhibited definite double eyelids and pore shrinkage, strongly indicating the active involvement of muscle, which was supported by an in vitro study. Our study also suggested the important role of biological factors delivered from injected stem cells, although the detailed mechanism of rejuvenation effects of ADSC skin injection remains to be clarified.

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Secretomes as an emerging class of bioactive ingredients for enhanced cosmeceutical applications

Cited by 14 publications

References 95 publications

Retrospectives on Three Decades of Safe Clinical Experience with Allogeneic Dermal Progenitor Fibroblasts: High Versatility in Topical Cytotherapeutic Care

Retrospectives on Three Decades of Safe Clinical Experience with Allogeneic Dermal Progenitor Fibroblasts: High Versatility in Topical Cytotherapeutic Care

Inhibition of LPS‑induced NLRP3 inflammasome activation by stem cell‑conditioned culture media in human gingival epithelial cells

A Single Intradermal Injection of Autologous Adipose-Tissue-Derived Stem Cells Rejuvenates Aged Skin and Sharpens Double Eyelids

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