Secretomic analysis of large cell lung cancer cell lines using two-dimensional gel electrophoresis coupled to mass spectrometry

Abstract

Abstract:The secretome of cancer cells is a valuable source of biomarkers that can ultimately reach the serum or other body fluids. Cancer biomarkers can facilitate early diagnosis and monitoring of the disease, contribute to our understanding of tumor biology, and support the development of more efficient therapies. Recently, high-throughput proteomic analysis of the conditioned media of cancer cell lines has shown potential to identify novel biomarkers in cancer. We used two-dimensional gel electrophoresis coupled to liquid chromatography tandem mass spectrometry to identify the secretome of the large cell lung cancer cell lines QU-DB and Mehr-80, which were established from a Canadian and a Persian patient, respectively. A total of 130 distinct protein species were identified. Most of them were previously found in serum or other body fluids, the membrane compartment or conditioned media of other cancer cell lines. Some of the proteins that we identified, e.g. IL-6, triosephosphate isomerase, PGP9.5, a-enolase, Dickkopf-1, and peroxiredoxin-1 have been already known as putative serum markers for lung cancer, whereas others might be candidate markers for further validation in lung cancer body fluids such as IL-25, stathmin, vimentin, peptidyl-prolyl cis-trans isomerase A, transgelin-2, and chloride intracellular channel protein 4.

Clinical Application of Mass Spectrometry‐Based Proteomics in Lung Cancer Early Diagnosis

“…In vitro studies [78][79][80][81]29,69,70] used similar approaches to analyze the proteome and secretomes of cell lines model for lung cancer (e.g., A549 cells derived from lung adenocarcinoma, or COR-L23 lung large cell carcinoma) compared to immortalized normal bronchial cell lines. However, the majority of the proteins found altered in lung cancer cell lines were more generically related to inflammation, or other non-cancer specific conditions.…”

“…Genesis, progression, recurrence, and metastasis of squamous cell carcinoma. [80,55,72,20,68] Complement factor Essential role in the activation of the innate immunity. Reflects progression of malignancy, including lung cancer.…”

Microfluidic perfusion systems for secretion fingerprint analysis of pancreatic islets: applications, challenges and opportunities

“…More information regarding islet protein profiling can be found in recent reports. 33,34 To date, determination of the secretome has focused mostly on cells such as lung, 35 colon 36 and glial 37,38 cancer cells. Determining such cancer cell secretomes has unveiled mechanisms related to angiogenesis, invasion and metastasis, helping to develop new strategies to control cancer.…”

Serum Levels of Fatty Acid Binding Protein 5 Decreased in Squamous Cell Lung Carcinoma

“…In lung cancer, controversy exists about over-expression or under-expression of FABP5 [18]. Its presence in lung cancer secretome has been reported [12]. To the best of our knowledge, serologic value of this molecule in lung cancer has not been investigated before.…”

“…Its overexpression has been linked to increased glycolysis and aggressiveness in cancer [10]. TPI presence in culture media of lung cancer cell lines and upregulation in tumor tissue have been frequently detected [11,12]. An elevated serum level of TPI in lung cancer was reported from Korea and China [11,13].…”