Secretomic insight into the biomass hydrolysis potential of the phytopathogenic fungus Chrysoporthe cubensis

Computational Tools and Phytopathogenic Fungi Secretome: Unraveling the Protein Arsenal

“…The CAZy database classifies the enzymes into families and subfamilies according to their sequence, structure, and biochemical information. Enzyme classes covered by CAZy include Glycosyl Hydrolase (GH), Carbohydrate Esterase (CE), Polysaccharide Lyase (PL), Glycosyltransferases (GT), and enzymes with Auxiliary Activity (AA) [9]. It is known that CAZYmes can be applied in various industrial processes, and many studies are showing the secretion of these enzymes of commercial interest by phytopatogenic fungi [7].…”

“…It is known that CAZYmes can be applied in various industrial processes, and many studies are showing the secretion of these enzymes of commercial interest by phytopatogenic fungi [7]. The xylanases, important CAZymes secreted by several pathogen fungus can be used for various biotechnology process such as clarification of juices, pulp bleaching, bioconversion of lignocellulose into fermentable sugars and preparation of animal food and have been reported in many papers [9,[13][14][15][16][17].…”

“…CAZymes and accessory enzymes produced by the pathogen fungus Penicillium funiculosum are distributed across different subfamilies (GH3, GH5, GH6, GH7, GH10, GH11, GH16, GH30, GH43, GH62, GH71, GH93, CE1, CE5, AA7 and AA9) that are responsible for biomass degradation and can be applied in several biotechnological applications [18]. Recently, our research group published data on the phytopathogenic fungi Chrysoporthe cubensis [9], Ceratocystis fimbriata [15] and Kretzschmaria zonata [16] that aroused interest in being able to secrete enzymes of great commercial appeal. Overall, studied fungi showed wide enzymatic diversity in their exoproteome, thus, the enzymes secreted by these fungi can act in several biotechnological processes, such as lignocellulosic biomass degradation and xylooligosaccharides production contributing with applicable resources in the bioenergy, food and pharmaceutical industries.…”

“…Furthermore, by using integrated proteomics with computational tools, it is possible to predict the subcellular location, when proteins are expressed, and the different proteins and enzymes produced by fungi. It is also possible to discover the implications for fungi development and changes in environmental responses [7][8][9]. Therefore, the enzymes secreted by phytopathogenic fungi deserve attention due to their potential for industrial application, which encourages research related to genetics and the secretion mechanisms of these enzymes [5].…”

Harnessing microbial wealth for lignocellulose biomass valorization through secretomics: a review

“…Since the strategy of using enzyme cocktails has proven to be more effective than the use of single enzymes in lignocelluloses degradation, the identification and characterization of enzymes from bacterial and fungal secretomes would be useful in formulating enzyme cocktails to accelerate lignocellulosic biomass degradation. In addition to the identification of novel enzymes/proteins from lignocellulolytic microbes (Table 1 ), secretome profiling of phytopathogenic microbes certainly offers the best opportunity to harness very active lignocellulolytic enzymes (Table 1 ) [ 142 ]. For example, LC–MS/MS-based identification of proteins present in the secretome of a phytopathogenic fungus Ustilago maydis showed the presence of carbohydrate-active enzymes (CAZymes), hydrolases and oxido-reductases.…”