Secretory Profiles of Pregnancy‐Associated Glycoproteins at Different Stages of Pregnancy in the Goat

González, F.; Sulon, Joseph; Garbayo, J. M.; Batista, M.; Cabrera, Fernando; Calero, P.; Gracia, A.; Beckers, J. F.

doi:10.1046/j.1439-0531.2000.00202.x

Cited by 21 publications

(20 citation statements)

References 13 publications

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“…However, it was surprising to find that PAG concentrations in pregnant buffalo cows were almost two to three times higher than those observed in dairy cows from day 30 to 60 after breeding (Perenyi et al 2002b;Lopez-Gatius et al 2007). A rapid increase in PAG concentrations associated with high maternal concentrations during early pregnancy is characteristic of caprine (Gonzalez et al 2000) and ovine species (Ledezma-Torres et al 2006). On the opposite, in cattle, concentrations increase slowly and remain at low levels during early pregnancy gestation (Zoli et al 1992b;Patel et al 1997;Perenyi et al 2002a).…”

Section: Discussionmentioning

confidence: 98%

“…2007). A rapid increase in PAG concentrations associated with high maternal concentrations during early pregnancy is characteristic of caprine (Gonzalez et al. 2000) and ovine species (Ledezma‐Torres et al.…”

Section: Discussionmentioning

confidence: 99%

“…By using biochemical procedures, several molecules of the PAG family were isolated from cotyledons of cow ), ewe (El Amiri et al 2003, 2004 and goat (Garbayo et al 1998). Purified and semi-purified preparations were used to immunize rabbits and the antisera obtained allowed the development of homologous (Sasser et al 1986;Zoli et al 1992b;Mialon et al 1993) and heterologous RIA systems (Ranilla et al 1994;Gonzalez et al 2000;Perenyi et al 2002a). By using these RIA systems, PAG profiles had been described in several ruminant species (Sasser et al 1986;Zoli et al 1992b;Ranilla et al 1994;Sousa et al 1999;Gonzalez et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

El-Battawy¹,

Sousa²,

Szenci³

et al. 2009

Reprod Domestic Animals

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Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F(2alpha) (Lutalyse) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 +/- 0.92 ng/ml) until day 41 (27.27 +/- 6.74 ng/ml), remaining high (20.71 +/- 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51-4.80 ng/ml) than in non-pregnant buffaloes (0.28-1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

El-Battawy¹,

Sousa²,

Szenci³

et al. 2009

Reprod Domestic Animals

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“…During pregnancy, the plasma ovPAG concentrations vary according to the breed of the ewe, the stage of pregnancy, and the number and genotype of the fetuses [13,14]. Moreover, the antiserum used in the radioimmunoassay of PAG is a source of variation for PAG concentrations in the same animal [15,16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of false transrectal ultrasonographic pregnancy diagnoses in sheep by measuring the plasma level of pregnancy-associated glycoproteins

Karen

Beckers²,

Sulon³

et al. 2003

Reprod. Nutr. Dev.

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-The present study was undertaken to investigate to what extent pregnancy diagnoses made by transrectal ultrasonography could be confirmed by measurements of plasma concentration of ovine pregnancy-associated glycoproteins (ovPAG). A total of 424 Awassi × Merino ewes were synchronized for estrus and examined by transrectal ultrasonography. In Experiment 1, the ewes (n = 156) were repeatedly scanned in a standing position on d 29, 36 and 50 of gestation. Similarly, the ewes (n = 268) in Experiment 2 were scanned on d 24, 29 and 34 of gestation, but these ewes were fasted for 12 h prior to the examination and the abdominal wall of each animal was lifted up by the hands of the assistant during the scanning. Blood samples were withdrawn after each transrectal ultrasonographic examination in both experiments. Ovine PAG concentrations were measured in plasma by a heterologous radioimmunoassay and the cut-off value for pregnancy was ≥ 1 ng·mL -1 . Based on the lambing performance, in Experiment 1, altogether 47 false negative and 38 false positive diagnoses were made by transrectal ultrasonography in 24 and 33 ewes, respectively between d 29 and 50 of gestation. In Experiment 2, altogether 8 false negative and 13 false positive diagnoses both were made in 7 ewes between d 24 and 34 of gestation. In both experiments, all ewes with false negative diagnoses had ovPAG concentrations higher than the threshold level for pregnancy diagnosis and all ewes with false positive diagnoses had ovPAG concentrations lower than the threshold of pregnancy. Furthermore, by the PAG-RIA test all lambed or aborted ewes (n = 63) were correctly diagnosed as pregnant and with three exceptions, all non-lambed ewes (n = 361) were correctly diagnosed as non-pregnant during the examined periods of both experiments.transrectal ultrasonography / ovPAG / pregnancy diagnosis / sheep Reprod. Nutr. Dev. 43 (2003) 577-586 577

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“…PAGs can be detected in the maternal blood circulation from embryo implantation (Gordon, 1999). Trophoblast placental cells are present in blood circulation during implantation until parturition and are responsible for producing PAGs throughout the gestation period (Gonzales et al, 2000). The protein concentration of PAG increases progressively at 3 and 4 months of gestation.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Pregnancy-Associated Glycoprotein as a Biomarker of Pregnancy in Etawa Crossbred Goat.

Ningtyas¹,

Lestari²,

Hermadi³

2019

jwpr

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Pregnancy-Associated Glycoprotein (PAG) is secreted by the placenta, which is produced in mononucleate and binucleate trophoblast cells. The current research was conducted to find out a substance for diagnosing early pregnancy in Etawa crossbred goats. Six Etawa crossbred goats (not pregnant, three months pregnant and four months pregnant) were subjected in the present study from Livestock Government Institution Breeding in Singosari, Malang. The research methods consisted of sample collection, identification PAG with sodium dodecyl sulfatepolyacrylamide gel electrophoresis, the determination of concentration with Biuret method and specificity test with Western Blot assay. The obtained results showed that the molecular weight of PAG from pregnant Etawa crossbred goats was 55.85 kDa. The average concentrations of PAG in the goats of non-pregnant, three months pregnant, and four months pregnant were 1.83±2.98, 3.79±2.72 and 4.36±2.63, respectively. The results of the specificity test with the Western Blot molecular revealed a molecular mass of PAG was 55 kDa. The findings of the present study demonstrated PAG in Etawa crossbred goats can be used as an indicator of pregnancy.

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Secretory Profiles of Pregnancy‐Associated Glycoproteins at Different Stages of Pregnancy in the Goat

Cited by 21 publications

References 13 publications

Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

Pregnancy‐associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

Evaluation of false transrectal ultrasonographic pregnancy diagnoses in sheep by measuring the plasma level of pregnancy-associated glycoproteins

Characterization of Pregnancy-Associated Glycoprotein as a Biomarker of Pregnancy in Etawa Crossbred Goat.

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