Secretory protein synthesis in parotid glands of young and old rats

Kim, S.-K.; Calkins, Debby W.

doi:10.1016/0003-9969(83)90019-5

Cited by 16 publications

(7 citation statements)

References 23 publications

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“…Age-associated decreases in the amount of c -amylase activity of rat parotid gland are accompanied by reduced levels of amino acid incorporation into total protein and, specifically, into aamylase (Kimetal., 1980;Kim, 1981;Baumetal., 1982). Amino acid incorporation into other secretary proteins is similarly reduced with age (Kim and Calkins, 1983). Restitution of secretary material by rat submandibular gland occurs more slowly following stimulation with isoproterenol in aged animals (Mysliwski, 1977).…”

Section: Discussionmentioning

confidence: 94%

Age-related Changes in Mucins from Human Whole Saliva

Denny

Klauser

et al. 1991

J Dent Res

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The predominant mucins in human whole saliva, MG1 and MG2, serve to protect and to lubricate the oral cavity. In this study, both unstimulated and stimulated whole salivas were collected from two groups of subjects: young (18-35 years of age) and aged (65-83 years of age). The subjects were in apparent good health. Saliva samples from each subject were analyzed by SDS-PAGE. The gels were stained with Stains-all, and both MG1 and MG2 were quantitated by video-image densitometry. The protocol gave reproducible values for each mucin. The stimulated and unstimulated salivas from aged subjects showed significant reductions in concentrations of both MG1 and MG2, as quantitated in mucin dye-binding units. Possible associations of these reductions with the aging process are discussed.

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Section: Discussionmentioning

confidence: 94%

Age-related Changes in Mucins from Human Whole Saliva

Denny

Klauser

et al. 1991

J Dent Res

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“…It has been reported that reductions occur in the amylase content in saliva (Meyer et al, 1937;Meyer and Necheles, 1940;Chilla et al, 1974), as well as the secretion and the rate of flow of saliva (Bertram, 1967). We have shown previously that the rate of protein synthesis declines with age in rat parotid glands 1980, Kim, 19811, and that this decline reflects the reduction in the synthesis of secretory proteins (Kim et al, 1982;Kim and Calkins, 1983). These age-related changes that affect the salivary content and the synthesis of protein suggest that alterations occur in the structural elements of the secretory acinar cells of the gland.…”

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confidence: 88%

Changes in the secretory acinar cells of the rat parotid gland during aging

Kim

1984

Anat. Rec.

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The secretory acinar cells of parotid glands from rats of varying ages have been examined by electron microscopy to determine what age-related changes occur in these cells. The most prominent change noted in these cells is the progressive increase in the amount of lipofuscin granules with age. Lipofuscin granules are membrane-bound structures consisting of lipids, other subcomponents, and a matrix. In addition, these cells contain lipid droplets that are not associated with any other components and tend to accumulate at the base of the cells in older rats. Also, many acinar cells in the glands of old rats contain altered secretory granules which appear to be in the process of degeneration. The accumulation of lipid and degenerating secretory granules appears to be related to the reduced level of cellular secretory activity in the glands of older rats. It is possible that these two types of inclusions contribute to the formation of lipofuscin granules. Lipofuscin and degenerating secretory granules are associated with acid phosphatase, which is demonstrated cytochemically, indicating that these granules are lysosomal structures.

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“…Studies of the rate of synthesis of secretory proteins or their messenger RNA (mRNA) indicate that changes affecting functions of salivary glands occur during aging. It previously has been shown that rates of synthesis of proteins decline progressively with age in the rat parotid Kim, 1981Kim, , 1985Kim and Arisumi, 1985;Kim et al, 1980;Koller et al, 1992) and submandibular glands Koller et al, 1992). In the rat parotid gland, the rate of synthesis of amylase declines with age; this decline is accompanied by a parallel reduction in the level of amylase mRNA (Kim et al, 1992;Koller et al, 1992).…”

Section: Protein Synthesismentioning

confidence: 99%

Structural and functional changes in salivary glands during aging

Kim

Allen

1994

Microscopy Res & Technique

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Various salivary glands in senescent humans and other animals have been examined extensively to characterize the structural and functional changes that occur during aging. Although a wide range of different structural changes, involving both the parenchymal and stromal tissues, have been described, it is unclear how any of these changes affects the function of the salivary glands. One major change in structure is the reduction in the volume of acini with a concomitant increase in the ductal volume. Despite this loss of functional acini, the salivary output and the contents seem to be unaltered, or minimally altered, due to aging. One consistent change observed in many salivary glands of aged animals is the decline in the rate of synthesis of proteins and their messenger RNA (mRNA). However, the salivary acinar cells from aged animals can synthesize secretory proteins at an elevated rate just as effectively as those from their younger counterparts in response to external stimuli, which are known to enhance the rate of protein synthesis. Thus, it appears that the salivary acinar cells, which remain structurally intact during aging, seem to retain their functional efficiency. Furthermore, these acinar cells, although reduced in number, are sufficient in quantity to carry out most of the salivary gland functions.

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Secretory protein synthesis in parotid glands of young and old rats

Cited by 16 publications

References 23 publications

Age-related Changes in Mucins from Human Whole Saliva

Age-related Changes in Mucins from Human Whole Saliva

Changes in the secretory acinar cells of the rat parotid gland during aging

Structural and functional changes in salivary glands during aging

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