Secretory proteins and sex peptides of the male accessory gland in Drosophila sechellia

Chen, P.S.; Balmer, J.

doi:10.1016/0022-1910(89)90133-9

Cited by 38 publications

(14 citation statements)

References 20 publications

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“…Indeed, this part of SP had been shown to be essential to elicit the two postmating responses [8,14]. It is also conserved in SP sequences of other Drosophila species [30–32] (T. Schmidt & E. Kubli, unpublished data). However, the N‐terminal parts of the mature peptides are different.…”

Section: Discussionmentioning

confidence: 99%

Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex‐peptide pheromone

Saudan

Hauck

Soller

et al. 2002

European Journal of Biochemistry

112

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We have characterized a glycosylated, 31 amino-acid peptide of 4932 Da isolated from Drosophila melanogaster males. The mature peptide contains a sugar moiety of 1184 Da at a NDT consensus glycosylation site and a disulfide bond. It is synthesized in the male ejaculatory duct via a 54 amino-acid precursor containing an N-terminal signal peptide and Arg-Lys at the C-terminus which is cleaved off during maturation. The gene contains an intron of 53 bp and is localized in the cytological region 99B of the D. melanogaster genome. The peptide is therefore named DUP99B (for ductus ejaculatorius peptide, cytological localization 99B). The C-terminal parts of mature DUP99B and D. melanogaster sex-peptide (ACP70A) are highly homologous. Injected into virgin females, DUP99B elicits the same postmating responses as sex-peptide (increased oviposition, reduced receptivity). These effects are also induced by de-glycosylated native peptide or synthetic DUP99B lacking the sugar moiety. Presence of the glycosyl group, however, decreases the amount needed to elicit the postmating responses. Homologies in the coding regions of the two exons of DUP99B and sex-peptide, respectively, suggest that the two genes have evolved by gene duplication. Thus, we consider these two genes to be members of the new sex-peptide gene family.

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Section: Discussionmentioning

confidence: 99%

Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex‐peptide pheromone

Saudan

Hauck

Soller

et al. 2002

European Journal of Biochemistry

112

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“…Structurefunction analysis of SP shows a C-terminal domain to be essential for both the behavioral and physiological responses (Schmidt et al 1993a). The importance of the Cterminal domain is also supported by strong conservation of C-terminal sequences between SPs from related species (Chen and Balmer 1989;Schmidt et al 1993b).…”

Section: Introductionmentioning

confidence: 90%

Ectopic expression of sex-peptide in a variety of tissues in Drosophila females using the P[GAL4] enhancer-trap system

1997

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Sex-peptide (SP), which is secreted by the accessory gland of Drosophila males, is transferred to the female during copulation, thereby reducing her sexual appetite (receptivity to males) and stimulating ovulation/oviposition. SP is known to be taken up into the hemolymph of mated females, but it is not clear whether there are two separate target tissues, for behavioral changes and ovulation or only one target for both responses. We have employed the GAL4-UAS system to express SP transgene constructs, both in different tissues and in different cellular components of virgin females. A cytoplasmic form of SP lacking a signal sequence did not evoke any responses, even when expressed ubiquitously. In contrast, a membrane-bound form of SP induced typical post-mating behavior, indicating that SP must be outside the cell in order to exert its biological effects. A total of 204 randomly selected P[GAL4] enhancer-trap lines were screened for their ability to induce SP responses in combination with the membrane-bound SP expressed under GAL4 control. Thirty-three lines were associated with both behavioral change and stimulated ovulation. No line was associated with only one of the two responses, implying that the SP target(s) mediating the two responses are either identical, very closely located, or present in two distinct tissues with a common set of genetic determinants. Western blot analysis of head, thorax, and abdominal extracts revealed that the biological activity was correlated with expression in the head fraction.

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“…Male accessory glands contain peptides which affect female reproductive behavior. Female sexual receptivity is diminished after mating (22) and oviposition is stimulated (22). Peptides responsible for these behaviors have been isolated from Drosophila species (22,23) and sequenced.…”

Section: Endogenous Insect Peptidesmentioning

confidence: 99%

“…Female sexual receptivity is diminished after mating (22) and oviposition is stimulated (22). Peptides responsible for these behaviors have been isolated from Drosophila species (22,23) and sequenced. In addition, a peptide with similar behavioral influences in a lepidopteran has been isolated from Helicoverpa zea (24).…”

Section: Endogenous Insect Peptidesmentioning

confidence: 99%

Use of Peptides in the Pursuit of Novel Pest Control Agents

Masler

1993

Natural and Engineered Pest Management Agents

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Economic plant crops come under attack from a variety of natural enemies including fungi, insects and other plants. Development of chemical pesticides is expensive, and will become more restricted as environmental concerns are recognized. Natural products, specifically peptides and neuropeptides, offer vast potential for exploitation as leads to pest control agents. Benefits from research into biologically active peptides and related factors include the necessity to investigate and understand, in detail, the physiological context in which the peptide operates. In addition, research on peptide biosynthesis, mode of action, and catabolism reveals numerous biochemical steps which may prove to be targets for control agents. The variety of candidates for naturally-based control agent development is extensive, including microbial iron binding systems, fungal toxins, arthropod venom components, insect immune peptides, neuropeptides and others. In this symposium, these endogenous molecules are discussed with regard to the physiological processes they regulate and potential consequences of their manipulation, the need to explore leads to control agents in a wide variety of organisms, and the need to examine emerging technologies in an effort to bring laboratory successes to the field.Biologically active peptides convey, within their structures, information derived from the genes coding for the peptides and the genes coding for the processing enzymes which are essential to the biogenesis of mature peptides. Peptides may be considered, then, as informational biomolecules which deliver messages to target receptors, resulting in defined physiological responses. The This chapter not subject to U.S.

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Secretory proteins and sex peptides of the male accessory gland in Drosophila sechellia

Cited by 38 publications

References 20 publications

Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex‐peptide pheromone

Ductus ejaculatorius peptide 99B (DUP99B), a novel Drosophila melanogaster sex‐peptide pheromone

Ectopic expression of sex-peptide in a variety of tissues in Drosophila females using the P[GAL4] enhancer-trap system

Use of Peptides in the Pursuit of Novel Pest Control Agents

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