Secretory response of the human pancreas to continuous intravenous infusion of pancreozymin-cholecystokinin (Cecekin).

Banwell, John G.; Northam, B. E.; Cooke, W. T.

doi:10.1136/gut.8.4.380

Cited by 35 publications

(11 citation statements)

References 21 publications

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“…When caerulein, dissolved in physiological saline, was given by intravenous infusion (1 ml./min for 30 min), it could be tolerated in doses up to 4-5 ng/kg/min, with larger doses the majority of subjects complained of nausea (Torsoli, personal communication). These side-effects were similar to those described after administration of cholecystokinin-pancreozymin (Bandwell, Northam & Cooke, 1967).…”

Section: General Effects In Unanaesthetized Animalssupporting

confidence: 83%

The actions of caerulein on the smooth muscle of the gastrointestinal tract and the gall bladder

Bertaccini

Endean

et al. 1968

British J Pharmacology

119

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1. In the intact conscious dog, caerulein causes emesis and evacuation of the bowel. The mean effective dose by the intravenous route is 0.4-0.5 jig/kg, and by the subcutaneous route 3-4 jg/kg. 2. The gall bladder in situ or as an isolated preparation is highly sensitive to caerulein. A few ng/kg injected intravenously are sufficient to stimulate the gall bladder in situ and less than 1 ng/kg per min is effective when infused intravenously. The isolated gall bladder is contracted by caerulein in concentrations as low as 0.03-2 ng/ml. Krebs solution. There is no tachyphylaxis but, generally, a good dose-response relationship. Hence the gall bladder, especially that of the guinea-pig, appears to be very suitable for the bioassay of caerulein and related peptides. 3. In situ, the musculature of the gastrointestinal tract is also highly sensitive to caerulein. Doses as low as 1-5 ng/kg, administered intravenously, have a spasmogenic action on jejunal loops of the dog, and slightly larger doses contract the small intestine of the cat. The stomach and the large intestine seem to be somewhat less sensitive to the polypeptide. Caerulein has a considerable spasmogenic action on the rat pylorus but relaxes the sphincter of Oddi of the guinea-pig. 4. Isolated preparations of the gastrointestinal tract are relatively insensitive to caerulein and tachyphylaxis occurs readily.5. Blockade with atropine produces different effects in different intestinal segments and in different animal species. The spasmogenic action of caerulein on the gall bladder is atropine-resistant. 6. The effects of caerulein are -similar to those of cholecystokininpancreozymin in the organs tested in situ or as isolated preparations. Caerulein, however, is always more potent than cholecystokinin-pancreozymin, even on a molar basis. Compared with caerulein, human gastrin I has negligible activity. 7. The possible use of caerulein in cholecystography is discussed.In a preceding paper the actions of caerulein on the systemic arterial blood pressure of some common laboratory animals were reported

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Section: General Effects In Unanaesthetized Animalssupporting

confidence: 83%

The actions of caerulein on the smooth muscle of the gastrointestinal tract and the gall bladder

Bertaccini

Endean

et al. 1968

British J Pharmacology

119

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“…Second, all hydrolytic products of digestion produced an immediate washout effect. Third, amino acids and micellar fatty acid both evoked a sustained secretion of enzymes which, in the case of amino acids, did not differ from the enzyme output, considered to be maximal, in response to pancreozymin infused by vein (11,15).…”

Section: Discussionmentioning

confidence: 93%

“…The highest enzyme output before the development, if any, of side-effects (abdominal pain, nausea, vomiting, diarrhea, or fever) was characterized and validated (11,15) as the maximal response. To study the possible systemic effect of amino acids on pancreatic enzyme output, the standard amino acid solution was given intravenously to three volunteers for 2 hr while simultaneous intraduodenal perfusion with isotonic saline was maintained.…”

Section: Introductionmentioning

confidence: 99%

Pancreozymin bioassay in man based on pancreatic enzyme secretion: potency of specific amino acids and other digestive products

Go¹,

Hofmann²,

Summerskill³

1970

J. Clin. Invest.

239

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AB S T R A C T The ability of products of digestion to stimulate pancreozymin secretion in man was investigated using a bioassay procedure, based on duodenal perfusion, which quantified the total outputs of pancreatic enzymes evoked by intraduodenal stimuli under steady-state conditions. Patterns of response resulting from physiologic intraduodenal concentrations of test material were basal output (with isotonic saline), washout of enzymes (with dextrose, micellar fatty acid, and amino acids), and sustained output of enzymes (with amino acids and micellar fatty acid). The sustained secretion of pancreatic enzymes found during the 2nd hr of perfusion and subsequently was characteristic of pancreozymin-induced secretion. The enzyme output in response to a mixture of essential and nonessential amino acids was significantly higher than that evoked by micellar fatty acid and was comparable with that resulting from the maximally tolerated dose of pancreozymin given by vein.Perfusion with essential amino acids caused enzyme outputs comparable to those induced by perfusion with the original amino acid mixture, whereas perfusion with nonessential amino acids had no effect. When the essential amino acids were tested individually, only phenylalanine, methionine, and valine caused significant increases in pancreatic enzyme output; the effect of tryptophan was indeterminate. However, the pancreatic enzyme output was less in response to these three essential amino acids than to mixtures containing all of them.

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“…Recently, the availability of cholecystokinin-pancreozymin (CCK-PZ) and derivatives or analogues has resulted in attempts to utilize the specific pancreatic secretion of enzymes into the duodenum to measure pancreatic exocrine function (Burton, Evans, Harper, Howat, Oleesky, Scott, and Varley, 1960;Sun, 1963;Banwell, Northam, and Cooke, 1967;Wormsley, 1969;Ribet, Duffaut, Vaysse, and Laval, 1972). However, for reasons ranging from psychological to anatomical, it is impossible to intubate the duodenum to obtain aspirate for analysis in some adults and children in whom investigation of pancreatic exocrine function is clinically necessary.…”

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confidence: 99%

Trypsin and chymotrypsin in duodenal aspirate and faeces in response to secretin and cholecystokinin-pancreozymin

Sale¹,

Goldberg²,

Þjóðleifsson³

et al. 1974

Gut

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SUMMARYThe secretion of trypsin and chymotrypsin into the duodenum in response to secretin and CCK-PZ has been compared with the faecal excretion of the enzymes following similar stimulation in 47 individuals undergoing clinical investigation. The faecal output of chymotrypsin correlated well, but trypsin less satisfactorily, with the secretion of the respective enzymes into the duodenum. The faecal excretion of chymotrypsin following stimulation with secretin and CCK-PZ can therefore be used as an index of pancreatic enzyme-secretory capacity.The secretion of bicarbonate into the duodenum in response to secretin is a satisfactory and widely used criterion for assessing the secretory capacity of the exocrine pancreas (Wormsley, 1972a) despite the assumed origin of the bicarbonate from the biliary system and duodenal mucosa as well as the pancreas.Recently, the availability of cholecystokinin-pancreozymin (CCK-PZ) and derivatives or analogues has resulted in attempts to utilize the specific pancreatic secretion of enzymes into the duodenum to measure pancreatic exocrine function (Burton, Evans, Harper,

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Secretory response of the human pancreas to continuous intravenous infusion of pancreozymin-cholecystokinin (Cecekin).

Cited by 35 publications

References 21 publications

The actions of caerulein on the smooth muscle of the gastrointestinal tract and the gall bladder

The actions of caerulein on the smooth muscle of the gastrointestinal tract and the gall bladder

Pancreozymin bioassay in man based on pancreatic enzyme secretion: potency of specific amino acids and other digestive products

Trypsin and chymotrypsin in duodenal aspirate and faeces in response to secretin and cholecystokinin-pancreozymin

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