Dental age assessment is one of the most reliable methods of chronological age estimation used for criminal, forensic and anthropologic purposes. Visual, radiographic, chemical and histological techniques can be used for dental age estimation. Visual method is based on the sequence of eruption of the teeth and morphological changes that are caused due to function such as attrition, changes in color that are indicators of aging. Radiographs of the dentition can be used to determine the stage of dental development of the teeth from initial mineralization of a tooth, crown formation to root apex maturation. Histological methods require the preparation of the tissues for detailed microscopic examination. The chemical analysis of dental hard tissues determines alterations in ion levels with age, whereas the histological and chemical methods are invasive methods requiring extraction/sectioning of the tooth. In this chapter, the different techniques and considered studies were overviewed in conjunction with their advantages and disadvantages. It needs to be taken into consideration that rather than restricting on one age estimation technique, using the other available techniques additionally and performing repetitive measurements may be beneficial for accurate age estimation.