Knowledge of the build-up and erosion behaviour of sewer sediment is necessary to facilitate better sediment management strategies. The dynamic behaviours of sediment in specific deep combined sewer overflow (CSO) chambers in Liuzhou, China, were studied through a field investigation in this paper. The results presented show that the chambers suffered from sediment problems of varying severity, and the severity depends on the dry weather flow rate. The sediment layers in chambers reached a certain balance with dry and wet weather flow rate, respectively. It can be classified as irreversible and reversible according to its mobility. The former has a low environmental impact because of its limited mobility and low organic content, and its increasing rate is extremely low year by year, whereas the latter has a highly pollutant content, and is the major cause of sewer odour and CSO pollution. The removal of this layer is seen to be a very important aspect of sewer sediment management.