Sedimentary records of environmental conditions retrieved from archeological sites provide valuable insight into the milieux of ancient humans and context to understand societal and cultural changes. At Jordan River Dureijat, an open-air site in Israel’s Hula Valley documenting the entire Epipaleolithic period as well as the Early Neolithic, sediments exposed on the walls of the excavation pit reveal a sequence of lacustrine deposits accumulated continuously between c. 21.1 and 11.3 cal ka BP near the southeast margin of Paleolake Hula. Through sediment-grain-size, geochemical, and paleontological analyses, we describe the nature of the Paleolake Hula from the Last Glacial Maximum (LGM) to the start of the Holocene. Until c. 17.2 cal ka BP, Paleolake Hula existed as a relatively large and well-buffered waterbody. A rapid and substantial drawdown of the lake occurred at 17.2 cal ka BP, followed by more frequent changes in the position of the shoreline in a smaller and shallower lake, resulting in the deposition of alternating near-shore and deeper lacustrine facies. Since the LGM, seven beds of structureless, silty sediments preserve archeological artifacts belonging to three Epipaleolithic cultures as well as the Early Neolithic pre-pottery Neolithic A culture. These sediments were deposited during phases of low lake level during which times humans waded into the shallow part of the lake, leaving behind stone and bone tools such as bladelets, lunates and burins, fishing hooks, line weights and net sinkers. Using radiocarbon-dated charcoal and a Bayesian statistical model, we produced a chronostratigraphic model for the archeological site, which enables the comparison of proxy paleoclimate records produced from this site’s sediments with regional archives as well as with global trends and changes in the Northern Hemisphere climate. Periods of low lake stands are correlated with the end of the LGM, Heinrich Event 1, and the beginning of the Younger Dryas Stadial. High water stands occurred contemporaneously with the peak of the LGM and during the Bølling–Allerød interstadial. This new water-level record from Lake Hula confirms that lake-level changes here broadly paralleled those of the Dead Sea and Sea of Galilee during the late Pleistocene, highlighting the importance of northern water sources to the overall water balance of the lakes along the Dead Sea Transform.