Sediminibacillus albus sp. nov., a moderately halophilic, Gram-positive bacterium isolated from a hypersaline lake, and emended description of the genus Sediminibacillus Carrasco et al. 2008

Abstract

Sediminibacillus albus sp. nov., a moderately halophilic, Gram-positive bacterium isolated from a hypersaline lake, and emended description of the genus Sediminibacillus Carrasco et al. A moderately halophilic, Gram-positive-staining, endospore-forming, rod-shaped and strictly aerobic bacterium, designated strain NHBX5 T , was isolated from Lake Nanhuobuxun in China. Strain NHBX5 T was able to grow at NaCl concentrations of 0-22 % (w/v) (optimally at 7 %, w/v), at pH 6.0-9.0 (optimally at pH 7.5) and at temperatures of 10-45 6C (optimally at 37 6C). The cell-wall peptidoglycan of strain NHBX5 T contained meso-diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 . The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and a glycolipid. The genomic DNA G+C content of strain NHBX5 T was 44.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NHBX5 T was most closely related to Sediminibacillus halophilus EN8d T (98.6 % gene sequence similarity). The level of DNA-DNA relatedness between strain NHBX5 T and S. halophilus CGMCC 1.6199 T was 34.6 %. Based on the data presented, strain NHBX5 T represents a novel species of the genus Sediminibacillus, for which the name Sediminibacillus albus sp. nov. is proposed. The type strain is NHBX5 T (5DSM 19340 T 5CGMCC 1.6502 T ). In addition, an emended description of the genus Sediminibacillus is presented.Moderately halophilic bacteria that can grow optimally in media containing salt concentrations of 3-15 % (w/v) make up an extremely heterogeneous group of microorganisms that are distributed widely in diverse saline habitats such as salt lakes, brines, saline soils, soda lakes, salted foods and some other hypersaline environments (Kushner, 1985;Ventosa et al., 1998). The genus Sediminibacillus, which belongs to this moderately halophilic group of bacteria, was first proposed by Carrasco et al. (2008) to accommodate moderately halophilic, facultatively anaerobic, Gram-positive, rod-shaped bacteria related phylogenetically to members of the genera Thalassobacillus and Halobacillus within the family Bacillaceae. The genus Sediminibacillus comprises the single species Sediminibacillus halophilus, the type strain of which was isolated from sediment of Lake Erliannor in Inner Mongolia, China. During the course of an investigation of the diversity of halophilic micro-organisms in Nanhuobuxun salt lake (36 u 439 14.70 N 95 u 429 410 E), a spore-forming, Gram-positive, moderately halophilic strain (NHBX5 T ) was isolated that appeared to represent a novel species of the genus Sediminibacillus. In this study, the phenotypic, chemotaxonomic and genotypic characteristics of this moderately halophilic strain are presented. Nanhuobuxun salt lake is located at 2700 m above sea level in the south-east Bieletan region of Qaidam basin of Qinghai province, China. It is a very typical chloride saline lake, with a high salinity...

Microbial survey of the mummies from the Capuchin Catacombs of Palermo, Italy: biodeterioration risk and contamination of the indoor air

“…The other 4.3% of actinobacterial sequences was related to an uncultured bacterial clone detected on mold‐colonized water‐damaged building materials, indicating a high abundance and variety of Actinobacteria in such environments (Schäfer et al ., ). The phylum Firmicutes was represented by the order Bacillales (28.3%), and most of the sequences affiliated with species of the genus Sediminibacillus (15.2%), isolated from hypersaline environments (Wang et al ., ); 6.5% of clones related to this order affiliated with alkaliphilic uncultured clones as well as with cultivated alkaliphilic bacilli. Alkaliphilic Bacillus species have important industrial applications due to their ability to produce alkaline enzymes such as protease and cellulase (Nogi et al ., ).…”

‘ Gracilibacillus phocaeensis ’ sp. nov., ‘ Sediminibacillus massiliensis ’ sp. nov. and ‘ Virgibacillus ndiopensis ’ sp. nov., three halophilic species isolated from salty human stools by culturomics

“…Strain Marseille-P3518 T exhibited a 97.4% sequence identity with Sediminibacillus albus strain NHBX5 (GenBank accession no. NR_044031.1) [8] , the phylogenetically closest species with standing in nomenclature ( Fig. 2 ), which putatively classifies it as a member of the genus Sediminibacillus within the family Bacillaceae in the phylum Firmicutes.…”

Isolation, molecular characterization and extracellular enzymatic activity of culturable halophilic bacteria from hypersaline natural habitats

“…In the current study, four moderately halophilic bacterial isolates were identified using 16S rRNA analysis, which is acknowledged as the method of choice for identifying novel isolates to the genus and particularly species level. The results were compared with those described in a range of identification schemes and the literature in general (Hotl et al 1994;Amoozegar et al 2003;Liu et al 2005;Tamegai et al 2006;Wang et al 2009;Guzmán et al 2010). The halotolerant bacteria, Halobacillus salinus isolated from a salt lake in Korea can, for example, grow at 23% NaCl (Echigo et al 2005).…”