Seed Aging: The Genome and Its Expression

Protein ligands of the human adenovirus type 2 outer capsid identified by biopanning of a phage-displayed peptide library on separate domains of wild-type and mutant penton capsomers.

“…at positions 287-290 in the penton base and at positions 268-271 in the fiber shaft (Roberts et al, 1986). This hypothesis was further investigated using fiber N-terminal mutant FiAT61.…”

“…Since this sequence was unique to the biopanned fiber and was never found among the phagotopes isolated with the penton base, it was assumed to be eluted from the fiber by direct competition with soluble penton base for the same complementary peptide domain (first mechanism described above). The RRVLLG motif, consensus to the RLSNLLG peptide in the Ad2 penton base sequence at position 254 (Roberts et al, 1986), would therefore correspond to the fiber binding site in the penton base protein.…”

“…The consensus phagotope found was RQXQZP, in which X was an aromatic residue (phenylalanine or tryptophan) and Z an amino acid residue with a short side chain (serine or alanine). The best fitting peptide sequence in the Ad2 penton base protein was the RQPFQE motif at position 264 (Roberts et al, 1986), in a region presenting a high probability of accessibility and antigenicity. dumb-bell shaped structure under the electron microscope (Pettersson, 1984;Ruigrok et al, 1990;Karayan et al, 1994).…”

Increased persistence of lung gene expression using plasmids containing the ubiquitin C or elongation factor1α promoter

“…The bi-cistronic vector, pCIKLux.IO was constructed from pCIKLux with an internal ribosome entry site (IRES) and the E4ORF3 gene inserted downstream of the luciferase gene as follows. A 601 bp (5′-NotI, 3′-blunt ended) PCR fragment containing the encephalomyocarditis virus IRES, amplified from plasmid pCITE-1 (Novagen, Madison, WI, USA) and a 349 bp (5′-blunt ended, 3′-NotI) PCR fragment containing E4ORF3 (34 707-34 326 bp of the adenovirus genome 31 ), amplified from adenovirus DNA, were ligated into plasmid pCIKLux digested with NotI. The IRES-E4ORF3 (IO) sequence could be released from pCIKLux.IO on a 900 bp NotI fragment.…”

Types of chromosomal aberrations at first mitosis in aged lettuce (lactuca sativa L.) seeds.

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Damage to the genome of the seeds induced during storage manifests as chromosomal aberrations in the first mitotic divisions during germination (see Roberts 1988) . By analogy with the work on ionising radiations in plants, many investigators in the past classified the ageing induced chromosomal aberrations observed in their studies into two broad categories chromosome and chromatid types, the former when the damage affected both the chromatids of the chromosome at the same location, and the latter when only one of the sister chromatids was affected at a given location.

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