Seed bio-priming with tri-species consortia of phosphate solubilizing rhizobacteria (PSR) and its effect on plant growth promotion

Abstract

Three potential rhizobacteria namely Burkholderia gladioli (MTCC 10216), Pseudomonas sp. (MTCC 9002) and Bacillus subtilis (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek ( Trigonella foenum-graecum L.) and tomato ( Lycopersicon esculentum L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7 th day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia.

Seed Priming: A Potential Supplement in Integrated Resource Management Under Fragile Intensive Ecosystems

“…In a P-deficient tropical soil, priming of mung bean seeds with 0.01 and 0.02% P enhanced the P uptake (Shah et al, 2012). Kumar et al (2020) reported maximum P solubilizing activity in a consortium of Burkholderia gladioli, Pseudomonas sp., and Bacillus subtilis, and the application of this consortium through seed biopriming improved the soil available P by 54%.…”

Aplikasi Konsorsium Endo-Rhizobakteri Untuk Meningkatkan Vigor Benih Padi Gogo Lokal

“…Kemampuan endo-rhizobakteri dalam meningkatkan kecepatan tumbuh relatif dan T 50 disebabkan oleh kemampuan agens hayati tersebut dalam memacu perkecambahan benih. Kumar et al (2020) melaporkan bahwa inokulasi campuran BPF + Azotobacter sp. + Azospirilum sp.…”

“…mampu meningkatkan indeks vigor benih secara signifikan dibandingkan dengan tanpa inokulasi (kontrol). Kumar et al (2020) melaporkan bahwa konsorsium mikroba juga mampu meningkatkan perkecambahan benih dan pertumbuhan tanaman fenugreek dan tomat. Lebih lanjut, Saputra et al (2020) melaporkan bahwa perlakuan campuran agens hayati mampu meningkatkan viabilitas dan vigor benih cabai rawit lokal.…”

Enhancing plant growth promoting rhizobacterial activities through consortium exposure: A review

“…For the last six years researchers are working on designing rhizobacterial consortium for enhancing plant growth, their yield of production, soil health, as a biocontrol agent, and stabilizing the rhizobacterial interactions ( Kamalnath et al, 2019 ; Swandi et al, 2019 ; Asyiah et al, 2020 ; Kumar et al, 2020 ; Shang and Liu 2020 ; Vaid et al, 2020 ; Redondo-Gómez et al, 2021 ; Karuppiah et al, 2022 ; Shabaan et al, 2022 ) In the current review, the authors have emphasized the role of rhizosphere microbes in PGP activities. Moreover, the authors have also highlighted the recent trends in PGPR-based research works.…”