Seed cryopreservation and micropropagation of the federally threatened species, Price’s potato-bean (Apios priceana B.L. Robins.)

Pullman, Gerald S.; Bai, Ke; Ruland, D.; Cruse-Sanders, J. M.; Boyd, Robert S.; Johnson, Shannon L.

doi:10.1007/s11627-019-09971-9

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…Preservation ex situ (off-site) can be used in part or in the entire population when preservation in situ presents insurmountable difficulties, usually resulting from a lack of complete control over the many factors which influence the survival of individuals and, therefore, the genetic makeup of the conserved population [ 10 , 11 ]. Different ex situ systems emerge as a complementary measure to preservation in situ, mainly aimed at protecting genetic material and ensuring its maintenance over time, in viable conditions and with original genetic characteristics [ 8 , 12 ].…”

Section: Preservation Of Plant Genetic Resourcesmentioning

confidence: 99%

“…Several regions, including South America and Oceania, face an increased hazard due to temperature change, generating global warming, which consequently causes the melting of the glacier poles, destabilizing various ecosystems, and extinguishing natural resources [ 7 ]. The predictions for the 1980s were that about 20,000 species of higher plants, medicinal plants, and forest trees would be in danger of extinction [ 8 ]. However, over the years, this problem has been exacerbated due to indiscriminate deforestation, overcollection, intensive farming, and pollution, causing a continuous depletion of genetic variability.…”

Section: Introductionmentioning

confidence: 99%

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Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

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Section: Preservation Of Plant Genetic Resourcesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

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“…In addition, in vitro culture helps maintain genetic variability, reducing genetic bottlenecks and increasing the adaptability of populations, which maintains diversity, reduces the risk of species extinction, and, thus, ensures the success of ex situ conservation efforts [26,27]. Micropropagation has been used successfully for a variety of species, from ornamentals to forest trees, as it produces homogeneous seedlings under sterile conditions.…”

Section: Discussionmentioning

confidence: 99%

“…The seedlings can be used to enhance existing populations of vulnerable species or to establish new cultivation sites for conservation or research purposes. Ex situ breeding experiments at such sites will, therefore, not affect the genetic diversity of plant populations in their natural habitats or reduce their evolutionary potential [26,27].…”

Section: Introductionmentioning

confidence: 99%

Seed Maturity and Its In Vitro Initiation of Chilean Endemic Geophyte Alstroemeriapelegrina L.

et al. 2022

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Alstroemeria pelegrina (A. pelegrina), a Chilean endemic, is considered vulnerable as its natural habitat is currently threatened. The decline in the reproductive capacity of the species due to anthropogenic impacts and climate change has made it imperative to address the problem by developing large-scale propagation methods. The objective of this study was to establish protocols for breaking the dormancy and in vitro germination of A. pelegrina seeds to speed up the germination and seedling production processes. The research began with morphological observations of the reproductive process, followed by in vitro sowing. The results showed that the seeds reached full maturity in 51 days, and physiological maturity in 41 days, at which point the seeds could be harvested for in vitro germination. The mechanical scarification pretreatment improves the in vitro germination rate to 96% and the germination time to 7 days, showing that the species is characterized by physical seed dormancy. On the other hand, if the seed coat incisions are deeper than 0.5 mm, 30% of the potential plants are lost due to embryo damage. The study provides scientific evidence for the feasibility of large-scale in vitro propagation of the species and establishes an efficient method of seedling production.

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Micropropagation, encapsulation, and conservation of Decalepis salicifolia, a vanillin isomer containing medicinal and aromatic plant

Rodrigues

Kumar

Gokul

et al. 2020

In Vitro Cell.Dev.Biol.-Plant

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Seed cryopreservation and micropropagation of the federally threatened species, Price’s potato-bean (Apios priceana B.L. Robins.)

Cited by 6 publications

References 26 publications

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Seed Maturity and Its In Vitro Initiation of Chilean Endemic Geophyte Alstroemeriapelegrina L.

Micropropagation, encapsulation, and conservation of Decalepis salicifolia, a vanillin isomer containing medicinal and aromatic plant

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