Seed Cryopreservation of Some Medicinal Legumes

Abstract

Seed survival after storage in liquid nitrogen (–196°C) was examined in 12 wild medicinal legume species occurred Far East of Russia. Dry seeds of all species survived cryostorage without loss of viability. Initial germinability varied from 3 to 85%. The stimulatory effect of cryogenic temperature on germination, with or without subsequent chemical scarification, was observed in all species studied with deep physical dormancy or heterogeneous levels of hardseededness. Frozen seeds demonstrated higher germination percentages (the percentage of germinated seeds) and germination rates (time for first seed to germinate (T0) and time required (in days) to reach 50% of the final germination percentage (T50)) than the control ones. The anomalous seedlings were not observed after storage of seeds in liquid nitrogen. This study shows that cryostorage may be successfully applied for conservation of native species without detrimental effects on germination and growth.

Tolerance to Desiccation and Cryopreservation of Seeds of Seven South American Ilex Species

“…Even more, as was observed in three species, cryostorage enhanced germination. Similar response was reported for several orchid seeds (Nikishina et al, 2007;Popov et al, 2004); for Pyrus communis (Reed et al, 2001), Halimium atriplicifolium, Helianthemum apenninum, Helianthemum squamatum (P erez-García and Gonz alez-Benito, 2008), Angelica ursina, Oxytropis chankaenis, Oxytropis retusa (Kholina and Voronkova, 2008), Elaeis guineensis (Camillo et al, 2009), Passiflora suberosa, and Passiflora edulis (Ara ujo et al, 2016); and for wild medicinal legume species (Kholina and Voronkova, 2012) after seed cryostorage. It has been suggested that LN exposure may enhance the germination of treated seeds by breaking seed dormancy or softening of the seed coat (Kholina and Voronkova, 2008;Reed et al, 2001).…”

Tolerance of jenipapo seeds to cryoprotectants and thawing after immersion in liquid nitrogen

“…The use of slow thawing of cryostored seeds without losses has been observed frequently, as presented by Farias et al (2006) in jatoba (Hymenaea courbaril L.), Rocha et al (2009) in four cotton cultivars (Gossypium hirsutum L.), and by Kholina & Voronkova (2012) in 12 native species from eastern Russia.…”

Approaches to gene pool conservation of medicinal plant Oxytropis lanata (Pall.) DC. (Fabaceae)

“…Растения O. lanata сформировали семена с довольно высокой степенью твердосемянности (табл. 3, контроль), близкой к таковой O. chankaensis, у которого всхожесть семян в контроле не превышала 6,0 % (Kholina, Voronkova, 2012;Voronkova, Kholina, 2010). Нарушение непроницаемости кожуры при обработке серной кислотой привело к активному прорастанию семян (табл.…”

“…Воздействие ультранизкой температуры с последующим размораживанием было аналогично воздействию серной кислоты и, по-видимому, также связано с повреждением семянной кожуры. Преодоление экзогенного типа покоя под действием сверхнизких температур отмечено ранее для видов Oxytropis (Kholina, Voronkova, 2012;Voronkova et al, 2008;Voronkova, Kholina, 2010) и других дикорастущих бобовых (Kholina, Voronkova, 2012;Pence, 1991;Perez-Garsia, 2008;Salomăo, 2002).…”