Seed Germination of Arbutus unedo, A. andrachne and Their Natural Hybrid A. andrachnoides in Relation to Temperature and Period of Storage

Bertsouklis, Κ.F.; Papafotiou, Μ.

doi:10.21273/hortsci.48.3.347

Cited by 15 publications

(10 citation statements)

References 19 publications

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“…syriaca (Thanos and Doussi 1995), Coridothymus capitatus, Origanum vulgare subsp. hirtum, Satureja thymbra (Estrelles et al, 2010), whereas 15°C was found to be more effective for germination of the Mediterranean species Dianthus fruticosus (Papafotiou and Stragas, 2009), Globularia alypum (Bertsouklis and Papafotiou, 2010), and all the Arbutus species found in Greece (Bertsouklis and Papafotiou, 2013).…”

Section: Resultsmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

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Section: Resultsmentioning

confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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“…pomifera (Thanos and Doussi, 1995), and a number of other Mediterranean species, as Arbutus unedo, A. andrachne, A. × andrachnoides (Bertsouklis and Papafotiou, 2013), Dianthus fruticosus (Papafotiou and Stragas, 2009), Sideritis athoa (Papafotiou and Kalantzis, 2009a), Lithodora zahnii (Papafotiou and Kalantzis, 2009b). (Thanos and Doussi, 1995;Kadis and Georghiou, 2010;Vlachou et al, 2019), and for the Mediterranean species of Fabaceae Anthyllis barba-jovis (Morbidoni et al, 2008;Trigka and Papafotiou, 2017).…”

Section: Germinationmentioning

confidence: 99%

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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“…(Flora Europaea, 1996) are native in the eastern Mediterranean region including Greece. All three of these species are very attractive for ornamental use (Cervelli et al, 2012;Bertsouklis and Papafotiou, 2013) and of high value in forestry for reforestations because of their ability for resprouting from the lignotuber after a fire (Konstantinidis et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of polyphenolic content and antioxidant capacity in fruits of Arbutus unedo, A. andrachne and their natural hybrid A.× andrachnoides

Bertsouklis

Daskalakis

Biniari

et al. 2021

Not Bot Horti Agrobo

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Fruits of the three Arbutus species native in the Eastern Mediterranean region, i.e. Arbutus andrachne, A. unedo and their natural hybrid A. × andrachnoides, collected from two different regions in Greece (Kalamos and Varympompi), were analysed comparatively in order to determine their phenolic profile and measure the antioxidant activity using two different single electron transfer-based assays, ferric reducing ability (FRAP) and 2,2-diphenylpicrylhydrazil radical scavenging capacity (DPPH). The pomological characteristics of the hybrid were similar to A. andrachne, while A. unedo had about 2.0-2.5 times larger fruits, with 20% more moisture being 175% heavier. All three species and their biotypes from the two areas had high phenolic content and antioxidant properties and were clearly pulled in different groups. Total flavanols scored a very strong correlation to antioxidant capacity. Highest total flavanols concentrations were found in A. andrachne fruits, while the hybrid had similar concentrations with A. unedo. In samples from Kalamos, concerning condensed tannins, total flavones, flavonols, phenolics and antioxidant capacity FRAP, as well as total anthocyanin and delphinidin 3-O-galactoside, the hybrid presented average values compared to the two parental species, while A. andrachne had the highest values. A strong correlation was found between ortho-diphenols and total soluble solids, as well as of total phenolics and flavonoids with total anthocyanins. The polyphenolic content and the antioxidant capacity varied in different biotypes. A. andrachne biotype from Kalamos showed the highest antioxidant capacity and anthocyanin potential. All A. unedo biotypes and A. × andrachnoides from Varympompi showed higher concentrations of ascorbic acid compared to the other biotypes.

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Seed Germination of Arbutus unedo, A. andrachne and Their Natural Hybrid A. andrachnoides in Relation to Temperature and Period of Storage

Cited by 15 publications

References 19 publications

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Comparative study of polyphenolic content and antioxidant capacity in fruits of Arbutus unedo, A. andrachne and their natural hybrid A.× andrachnoides

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